FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697491
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTACTTTACCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCAT65080.38338936701284426No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58320.3435658863581604No Hit
GTACTACTCTGATGACAGTAATAGGTTGCAGCATCTTCAGCTTCCAGATTATTGATGGTGAGGGTGAAATCTGTC48750.2871885624135857No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG46780.2755831989683598No Hit
GACTTACACTGGTACCAGCAGAAACCACATCAGTCTCCAAAGCTCCTCAT46020.2711060029184249No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44150.26008974421661146No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42310.2492502169378218No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41630.24524430468261688No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA35630.20989802007786787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34820.20512627165622674No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34030.20047234418326815No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32310.1903397425965734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32220.1898095483275022No Hit
CTATTACTGTCATCAGAGTAGTACTTTACCATTCACTTTCGGCCCTGGGA32010.18857242836633595No Hit
GATTTCACCCTCACCATCAATAATCTGGAAGCTGAAGATGCTGCAACCTATTACTGTCATCAGAGTAGTACTTTA28890.1701923603718665No Hit
GTAAAGTACTACTCTGATGACAGTAATAGGTTGCAGCATCTTCAGCTTCCAGATTATTGATGGTGAGGGTGAAAT28580.16836613566728778No Hit
GTACTTTACCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGA28540.16813049376992278No Hit
CAGTAATAGGTTGCAGCATCTTCAGCTTCCAGATTATTGATGGTGAGGGT28150.1658329852706141No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28100.16553843289890785No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28020.16506714910417786No Hit
TCTCAGGGGTCCCCTCGAGGTTCAATGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAATCTGGAAG27960.1647136862581304No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27630.1627696406048692No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26690.15723205601679185No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC26620.15681968269640312No Hit
ACCTATTACTGTCATCAGAGTAGTACTTTACCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAA26050.15346178565895194No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25640.15104645621096074No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25350.14933805245506457No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC25340.1492791419807233No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25300.14904350008335832No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24760.1458623344689309No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24580.14480194593078843No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24570.1447430354564472No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24380.14362373644396348No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24110.14203315363674976No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT23760.13997128703480607No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG23140.13631883762564867No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCC22630.13331440343424503No Hit
GTGTAAGTCACTACCAATGGTCTGACTGGCCCGGCAGGTGATGGTGACTT22140.13042779019152384No Hit
CTCCAGGGGTGAGATTGTGCTGACTCAGTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCAC21600.12724662457709643No Hit
GTAATAGGTTGCAGCATCTTCAGCTTCCAGATTATTGATGGTGAGGGTGA21240.1251258475008115No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20580.1212377561942891No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC20460.12053083050219413No Hit
GGGTGAGATTGTGCTGACTCAGTCTCCAGACTTTCAGTCTGTGACTCCAA20320.11970608386141664No Hit
TCCCAGCCCTTCTCAGGGGTCCCCTCGAGGTTCAATGGCAGTGGATCTGG20230.11917588959234542No Hit
GGATTGGATTGGGTACATCCATCACAATGGGAGCACCAACTACAACCCCTCTTTCAAGAGTCGAGTCACCATAT20070.11823332200288543No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT20010.11787985915683794No Hit
CCCCTGAGAAGGGCTGGGAAGCATACTTGATGAGGAGCTTTGGAGACTGA18840.11098733365891189No Hit
CTCCAAAGCTCCTCATCAAGTATGCTTCCCAGCCCTTCTCAGGGGTCCCC18680.1100447660694519No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18610.10963239274906318No Hit
GGGTTCCAGCCTCCAGGGGTGAGATTGTGCTGACTCAGTCTCCAGACTTT18570.10939675085169817No Hit
GACTTACAGCAGCTCGTCAGGAAAGACCTTTGACTACTGGGGCCAGGGAA18330.10798289946750822No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18240.107452705198437No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18190.10715815282673076No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCCGAGC18190.10715815282673076No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17890.10539083859649329No Hit
GTTCAATGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAATC17770.10468391290439831No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17770.10468391290439831No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17760.10462500243005708No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG17650.10397698721230333No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT17580.1035646138919146No Hit
GCTGTAAGTCCTCGCACAATAATAAACGGCCGTGTCTGCGGCGGTCACAG17520.1032111510458671No Hit
GGGTGAAATCTGTCCCAGATCCACTGCCATTGAACCTCGAGGGGACCCCT17350.10220967298206589No Hit
GTGCTGACTCAGTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGT17300.10191512061035965No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17300.10191512061035965No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT17140.10097255302089966No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17080.10061909017485218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.041.662471
GTGGTAT5000.041.5271761
CGGTCAA350.001446063538.8692449
GTATCAA41650.037.5121
TGGTATC5750.036.0967522
TATCAAC47200.032.655952
ATCAACG47550.032.330683
TCAACGC48200.031.8946864
CAACGCA49050.031.480665
AACGCAG49100.031.379336
TACGTAT850.004394455330.98489870
GACTAGC450.004937960830.2565921
ACGCAGA51600.029.8590167
CGCAGAG51650.029.7642618
AGAGTAC52250.029.3565111
GCAGAGT53800.028.5739549
TTCCGTG605.8950554E-428.3429957
TCCGAGA8100.026.82488670
TCAGGTC15400.026.72103512
CTGGCAA16100.026.56409362
Produced by FastQC (version 0.11.5)