FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815609
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTTCTGTGGCGTTCGGGCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT36520.4477635729865659No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA30480.37370848041156973No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC27210.3336157398949742No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24560.30112468106654044No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23260.28518567107523335No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20580.25232678893930793No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT19290.23651038671716473No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17300.21211144065354845No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15880.19470113743227455No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT15260.18709945574411269No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14250.1747160710585587No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14240.1745934632893948No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13480.16527527283293833No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13200.16184225529634913No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCGCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA12670.15534404353066236No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11590.1421024044609611No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATACA11470.14063111123099425No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11450.14038589569266646No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11280.13830156361688015No Hit
GTTTATTACTGTCAGCAGTATTATAGTACTTCTGTGGCGTTCGGGCAAGGGACCAAGGTGGAAATCAAACGAA10710.13131292077453782No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10590.129841627544571No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10560.12947380423707927No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10520.1289833731604237No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10380.12726686439212906No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10260.12579557116216225No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10190.12493731677801496No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10050.12322080800972035No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10040.12309820024055645No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC9530.11684520401319749No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9480.116232165167378No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9210.11292175539995267No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGCCCGAACGCC8900.10912091455587175No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8660.10617832809593813No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC8200.1005383707143987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCT200.006539806353.31611653
CCGTCAT607.487846E-444.42470670
GGTATCA10450.042.5695461
CACGAAT456.058575E-541.74408766
GTATCAA28750.040.9099541
GAATACG1450.037.1552669
ATACGTA1500.035.91455511
CGTACCA1550.034.7538914
ACGTACC1550.034.7538913
GTGGTAT3300.034.722131
TGGTATC3300.034.7136082
ATCAACG34950.033.238423
TATCAAC35600.033.0301252
TCAACGC35400.033.0041054
AACGCAG35500.032.725466
CAACGCA35850.032.4998935
CGCAGAG37500.030.9801038
AATACGT1750.030.78579310
ATGGCAA3250.030.75556670
GCAGAGT37950.030.6108749
Produced by FastQC (version 0.11.5)