FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315300
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50840.3865277883372614No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46660.35474796624344257No Hit
GTATTACTGTGCTAGAGTCAGTATGGAGGCTGGTTATTTGTCCTACTATGACTACTGGGGCCAGGGAACCCTGGT39460.3000076028282521No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTTACCA37930.28837527560252413No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36320.27613472211662743No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32730.24884056869155327No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATT31960.24298639093742871No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG31490.2394130616589371No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30550.23226640310195393No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29620.22519577282749184No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29120.22139435870143692No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27130.20626473047973848No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26360.20041055272561392No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT26040.1979776476849388No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25840.1964570820345168No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25520.19402417699384172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25400.19311183760358852No Hit
CTACATGGACTGGGTTCGCCAGGTGCCAGGGAAGGGGCTGGAGTGGGTTG25370.19288375275602526No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25330.19257963962594085No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTA24950.18969056489013914No Hit
CAGTAATACAGGGCCGTGTCCTCGGTTTTCAGGCTATTCAGTTGCAGAAA23680.18003497300995971No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23430.17813426594693227No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23260.17684178514407362No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23080.17547327605869384No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGAGGCCCTGTT22920.17425682353835625No Hit
GACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTCCTGTCAGCAGCGTAGCTACTGG22400.17030335284725917No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21990.16718619326389417No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21770.16551357104843No Hit
GTTTATTCCTGTCAGCAGCGTAGCTACTGGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21450.1630806660077549No Hit
GTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG21380.1625484680301072No Hit
GTTATAATACAGAATACGCCGCGTCTGTGAGAGGCAGATTCACCATCTCA21180.16102790237968523No Hit
GTAATACAGGGCCGTGTCCTCGGTTTTCAGGCTATTCAGTTGCAGAAACA20300.15433741351782862No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19510.1483311791986619No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19100.1452140196152969No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC18520.1408043792290732No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18380.13973998327377785No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18200.13837147418839807No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18160.13806736105831371No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18140.1379153044932715No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC17940.13639473884284956No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17860.13578651258268076No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17590.13373374895461113No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17560.1335056641070478No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT17510.13312552269444233No Hit
GCGTAGCTACTGGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT17170.130540561088725No Hit
TCTATGATGCATCCAACAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT17150.1303885045236828No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17110.13008439139359843No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17000.12924808028586635No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16930.12871588230821868No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16920.12863985402569755No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16860.12818368433057098No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16850.12810765604804988No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA16790.12765148635292328No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16690.1268912035277123No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTTGGTTTTC16670.1267391469626701No Hit
CAACAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG16580.12605489241998025No Hit
GGAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16500.12544666615981145No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16420.12483843989964268No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16360.12438227020451609No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16270.12369801566182619No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCTCTGGCATCCCAGCCAGGTT15880.12073291264350339No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15610.11868014901543374No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15560.11830000760282824No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15560.11830000760282824No Hit
CTGTATTACTGTGCTAGAGTCAGTATGGAGGCTGGTTATTTGTCCTACTA15450.11746369649509618No Hit
CTGCTACTCTGGCTCCCAGTTACCACCGGAGAAGTTGTGTTGACACAGTC15340.11662738538736411No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15310.11639930053980081No Hit
GTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC15130.11503079145442105No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15020.11419448034668896No Hit
CATCTATGATGCATCCAACAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14930.11351022580399908No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14760.11221774500114043No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14730.11198966015357713No Hit
GCCCTGTATTACTGTGCTAGAGTCAGTATGGAGGCTGGTTATTTGTCCTACTATGACTACTGGGGCCAGGGAACC14700.11176157530601383No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14700.11176157530601383No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14480.1100889530905497No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC14320.10887250057021211No Hit
GTCCATGTAGTGGTCACTAAAAGTGAATCCAGAGGCTGCACAGGAGAGTC14300.10872044400516993No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14240.10826427431004333No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGG14220.10811221774500114No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14190.10788413289743784No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14110.10727590663726908No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCTCTGGCATCCCA13990.10636356724701589No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13900.10567931270432601No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13830.10514711472667831No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13720.10431080361894625No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13710.10423477533642515No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13680.10400669048886185No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13640.10370257735877746No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13620.10355052079373527No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13540.10294229453356649No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13380.10172584201322893No Hit
GTATGGAGGCTGGTTATTTGTCCTACTATGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCC13380.10172584201322893No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13370.10164981373070783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGT200.007670793751.2101423
CCTAACG200.007685851.184832
TCGTATG2500.051.04575340
AATCTCG2150.049.54183636
CTAATCT2200.046.78602234
CGTATGC2750.046.46415341
CTTATAC2950.046.2740481
GGTATCA9550.046.09841
GTGGTAT2450.045.967131
ATCGTAT306.344423E-445.95184739
ACGAGAC2850.045.51839422
GCCGTCT2800.044.65363746
ATGCCGT2950.044.64099544
CGAGACT2900.043.5580523
TCTCCGA2900.043.53652211
TTATACA3200.042.6540222
GCTAATC2500.042.5180833
CCGTCTT3000.041.73001547
CACGAGA3050.041.41112521
CCCACGA3050.041.39380619
Produced by FastQC (version 0.11.5)