FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315300
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50730.3856914772295294No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38270.2909602372082415No Hit
GTATTACTGTGCTAGAGTCAGTATGGAGGCTGGTTATTTGTCCTACTATGACTACTGGGGCCAGGGAACCCTGGT37190.2827491826959629No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36960.28100053219797766No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTTACCA35310.26845586558199647No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34200.26001672622215466No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATT30160.22930130008363112No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG29170.22177450011404243No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29130.22147038698395802No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28140.21394358701436936No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28010.21295521934159506No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26540.20177906181099367No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTA25820.19630502546947462No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25710.19546871436174257No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24880.18915836691249144No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT24480.18611723561164753No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23630.1796548315973542No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23560.17912263361970654No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23430.17813426594693227No Hit
GTTTATTCCTGTCAGCAGCGTAGCTACTGGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA22520.17121569223751237No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22290.1694670417395271No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21990.16718619326389417No Hit
CTACATGGACTGGGTTCGCCAGGTGCCAGGGAAGGGGCTGGAGTGGGTTG21730.16520945791834563No Hit
GACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTCCTGTCAGCAGCGTAGCTACTGG21510.1635368357028815No Hit
CAGTAATACAGGGCCGTGTCCTCGGTTTTCAGGCTATTCAGTTGCAGAAA21420.16285258116019158No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21260.16163612863985402No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21040.15996350642438986No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21000.15965939329430548No Hit
GTTATAATACAGAATACGCCGCGTCTGTGAGAGGCAGATTCACCATCTCA20740.15768265794875694No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGAGGCCCTGTT20630.15684634684102486No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20020.1522086216072379No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19900.1512962822169847No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19710.14985174484908387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19450.1478750095035353No Hit
GTAATACAGGGCCGTGTCCTCGGTTTTCAGGCTATTCAGTTGCAGAAACA19400.14749486809092985No Hit
GTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG19270.14650650041815555No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT18870.14346536911731164No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC18850.14331331255226945No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC18240.13867558731848248No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17780.13517828632251197No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17760.13502622975746978No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17730.1347981449099065No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17520.13320155097696343No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17170.130540561088725No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16730.1271953166577967No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA16710.12704326009275452No Hit
CTGCTACTCTGGCTCCCAGTTACCACCGGAGAAGTTGTGTTGACACAGTC16290.12385007222686839No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16120.12255759142400972No Hit
GCGTAGCTACTGGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT16100.12240553485896753No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16000.12164525203375656No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15860.1205808560784612No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15840.12042879951341899No Hit
CTGTATTACTGTGCTAGAGTCAGTATGGAGGCTGGTTATTTGTCCTACTA15630.11883220558047594No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15580.11845206416787044No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCTCTGGCATCCCAGCCAGGTT15510.11791986619022277No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC15430.11731163993005397No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15410.11715958336501178No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15410.11715958336501178No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15250.11594313084467421No Hit
GTGTTAGCAGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC15210.11563901771458981No Hit
CAACAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG15100.11480270660685775No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTTGGTTTTC15080.11465065004181556No Hit
TCTATGATGCATCCAACAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14860.1129780278263514No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14800.11252185813122483No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14740.11206568843609822No Hit
GGAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14590.11092526419828176No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14560.11069717935071846No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14450.1098608682429864No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14310.10879647228769103No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14270.10849235915760663No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14260.10841633087508554No Hit
GCCCTGTATTACTGTGCTAGAGTCAGTATGGAGGCTGGTTATTTGTCCTACTATGACTACTGGGGCCAGGGAACC14160.10765604804987454No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14080.10704782178970577No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14040.10674370865962138No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14010.10651562381205808No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCTCTGGCATCCCA13990.10636356724701589No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13940.10598342583441041No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13920.10583136926936822No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13920.10583136926936822No Hit
CATCTATGATGCATCCAACAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13750.10453888846650955No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13720.10431080361894625No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13640.10370257735877746No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13260.10081350262297574No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC13170.10012924808028587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAT405.3744185E-542.603914
GTGGTAT2950.041.6214521
GGTATCA10050.041.0635071
TGGTATC3550.035.5286672
GTATCAA22750.035.380781
GACAGGT2500.032.7422071
TCTTGAG10650.031.04742270
TATCAAC26100.030.9537542
CGCACGT450.004907056730.2949614
ATCAACG27150.029.7509923
AACGCAG27450.029.5500036
TCAACGC27500.029.4973984
AATGGTA3051.8189894E-1229.36144870
CAACGCA27750.029.2305435
TGTTAGA12600.028.97605370
CTTCCAT3101.8189894E-1228.88787370
ACGCAGA28750.028.2138297
CGCAGAG29000.027.9706048
AGAGTAC29150.027.70975711
GCAGAGT29750.027.2654679
Produced by FastQC (version 0.11.5)