FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802077
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72470.4021470780660316No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71510.396819891713839No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54390.30181840176640623No Hit
GAATTCACTCTCACCATCACCAGCCTGCAACCTGACGATTATGCAACTTACTACTGTCATCAAAGTTACAGTTCC52070.288944368081941No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46800.25970033466938425No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45840.25437314831719177No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG45800.25415118221918376No Hit
ACTTACTACTGTCATCAAAGTTACAGTTCCCCTCCGCTCAGTTTCGGCGG45780.2540401991701797No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44300.245827453543883No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC43390.2407777248142005No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41210.22868057247276335No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41140.22829213180124933No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40410.22424125051260296No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG40250.2233533861205709No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39360.21841464043989242No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38100.2114227083526398No Hit
ACACAAACCTGGGAAAGCCCCCAAACTCCTGATTTTTGCTACGTCCTCATTGGAAATGGGGATCCCACCCAGGTT37640.2088700982255475No Hit
CACCATCACCAGCCTGCAACCTGACGATTATGCAACTTACTACTGTCATC37060.2056515898044312No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG35240.1955521323450663No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCATCCAGCTGACCCAGTC34060.18900413245382966No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGAGGTCAACGGTGGTCC32310.17929311566597875No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31510.17485379370581833No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30920.17157979376020005No Hit
GTAGTAAGTTGCATAATCGTCAGGTTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTGCCAGATCCGCCGCCA30430.1688607090596018No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG29090.1614248447763331No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28910.160425997335297No Hit
GAGTGAATTCTGTGCCAGATCCGCCGCCACTGAACCTGGGTGGGATCCCC28390.15754043806119272No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28280.15693003129167066No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28150.15620864147314462No Hit
CAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT27940.1550433194586025No Hit
GTTCCCCTCCGCTCAGTTTCGGCGGGGGGACCACCGTTGACCTCAAAGGA27350.1517693195129842No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26960.149605150057406No Hit
AAGTTACAGTTCCCCTCCGCTCAGTTTCGGCGGGGGGACCACCGTTGACCTCAAAGGAACTGTGGCTGCACCAT26070.14466640437672754No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25860.14350108236218542No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25290.14033806546557112No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25040.13895077735302097No Hit
GACTATTGGCGGCCACAGCGGTCCCTATTTAAATTGGTATCAACACAAAC25010.13878430277951498No Hit
GTTTCGGCGGGGGGACCACCGTTGACCTCAAAGGAACTGTGGCTGCACCA24550.13623169265242271No Hit
GAGGGGAACTGTAACTTTGATGACAGTAGTAAGTTGCATAATCGTCAGGT24430.13556579435839866No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCAT23980.13306867575580844No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23110.128240913124134No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23090.12812993007513No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCATCC22920.12718657415859588No Hit
GTGCCAGATGTGGCATCCAGCTGACCCAGTCTCCAGCTTTTTTGTCTGCC22840.12674264196257984No Hit
GGTTTGTGTTGATACCAATTTAAATAGGGACCGCTGTGGCCGCCAATAGT22780.1264096928155678No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22750.1262432182420618No Hit
CCATTGATTGCCGGGCTAATCAGACTATTGGCGGCCACAGCGGTCCCTAT22710.12602125214405377No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22640.12563281147253974No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22580.12529986232552773No Hit
CCGTTGACCTCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG21770.12080504884086529No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21500.11930677767931115No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21410.11880735395879309No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21310.11825243871377304No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21190.11758654041974899No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21190.11758654041974899No Hit
GTGCAGCCACAGTTCCTTTGAGGTCAACGGTGGTCCCCCCGCCGAAACTG21040.11675416755221892No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20470.11359115065560461No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20250.1123703371165605No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20190.11203738796954847No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20140.11175993034703843No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19580.10865240497492615No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19560.10854142192592214No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19470.1080419982054041No Hit
CAGTAGTAAGTTGCATAATCGTCAGGTTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTGCCAGATCCGCCGCCA19250.10682118466635998No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19200.10654372704384996No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19180.10643274399484595No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18770.10415759149026373No Hit
GTTCCTTTGAGGTCAACGGTGGTCCCCCCGCCGAAACTGAGCGGAGGGGA18620.10332521862273367No Hit
CCAATAGTCTGATTAGCCCGGCAATCAATGGTGACTGTGTCTCCCACAGA18620.10332521862273367No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18440.10232637118169756No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18410.10215989660819155No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18400.10210440508368955No Hit
AATCAGACTATTGGCGGCCACAGCGGTCCCTATTTAAATTGGTATCAACACAAACCTGGGAAAGCCCCCAAACTC18390.10204891355918755No Hit
GGTTCAGTGGCGGCGGATCTGGCACAGAATTCACTCTCACCATCACCAGC18230.10116104916715546No Hit
GTGGGAGACACAGTCACCATTGATTGCCGGGCTAATCAGACTATTGGCGG18150.10071711697113941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)