FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802077
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69570.3860545359604501No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59840.33206128261999907No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55620.3086438592801528No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53000.2941050798606275No Hit
GAATTCACTCTCACCATCACCAGCCTGCAACCTGACGATTATGCAACTTACTACTGTCATCAAAGTTACAGTTCC50180.27845646995106205No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG48720.27035470737376927No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46910.2603107414389063No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44930.2493234195875093No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43010.23866904688312432No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41290.22912450466877943No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT41030.2276817250317273No Hit
ACTTACTACTGTCATCAAAGTTACAGTTCCCCTCCGCTCAGTTTCGGCGG40750.22612796234567112No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39350.2183591489153904No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC38240.21219958969566785No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG37730.2093695219460656No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36940.20498569151040716No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34110.1892815900763397No Hit
ACACAAACCTGGGAAAGCCCCCAAACTCCTGATTTTTGCTACGTCCTCATTGGAAATGGGGATCCCACCCAGGTT33450.18561914945920735No Hit
CACCATCACCAGCCTGCAACCTGACGATTATGCAACTTACTACTGTCATC33340.18500874268968528No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCATCCAGCTGACCCAGTC32380.17968155633749278No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31780.17635206486737248No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30660.1701370141231479No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30630.1699705395496419No Hit
GAGTGAATTCTGTGCCAGATCCGCCGCCACTGAACCTGGGTGGGATCCCC29910.1659751497854975No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT29860.1656976921629875No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29760.16514277691796744No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGAGGTCAACGGTGGTCC29750.16508728539346543No Hit
GTAGTAAGTTGCATAATCGTCAGGTTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTGCCAGATCCGCCGCCA29020.16103640410481904No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG28640.15892772617374284No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27950.1550988109831045No Hit
GTTCCCCTCCGCTCAGTTTCGGCGGGGGGACCACCGTTGACCTCAAAGGA26050.1445554213277235No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25970.14411148913170746No Hit
AAGTTACAGTTCCCCTCCGCTCAGTTTCGGCGGGGGGACCACCGTTGACCTCAAAGGAACTGTGGCTGCACCAT25610.14211379424963527No Hit
GAGGGGAACTGTAACTTTGATGACAGTAGTAAGTTGCATAATCGTCAGGT25530.14166986205361923No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24940.13839586210800092No Hit
GTGGGAGACACAGTCACCATTGATTGCCGGGCTAATCAGACTATTGGCGG24920.13828487905899692No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24040.13340162490282048No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24000.13317965880481245No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24000.13317965880481245No Hit
GTTTCGGCGGGGGGACCACCGTTGACCTCAAAGGAACTGTGGCTGCACCA23900.1326247435597924No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23880.1325137605107884No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23800.13206982831477235No Hit
CAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT23780.13195884526576832No Hit
GACTATTGGCGGCCACAGCGGTCCCTATTTAAATTGGTATCAACACAAAC23770.13190335374126633No Hit
GGTTTGTGTTGATACCAATTTAAATAGGGACCGCTGTGGCCGCCAATAGT23670.13134843849624628No Hit
GTGCCAGATGTGGCATCCAGCTGACCCAGTCTCCAGCTTTTTTGTCTGCC22850.12679813348708185No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCAT21920.12163742170839538No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21770.12080504884086529No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21400.11875186243429109No Hit
GTGCAGCCACAGTTCCTTTGAGGTCAACGGTGGTCCCCCCGCCGAAACTG21250.11791948956676102No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCATCC21230.11780850651775701No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21160.11742006584624298No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21090.11703162517472895No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20670.11470098114564473No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20570.11414606590062466No Hit
CCGTTGACCTCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20500.11375762522911063No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20470.11359115065560461No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20210.11214837101855249No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20130.11170443882253644No Hit
CCATTGATTGCCGGGCTAATCAGACTATTGGCGGCCACAGCGGTCCCTAT19470.1080419982054041No Hit
GGTTCAGTGGCGGCGGATCTGGCACAGAATTCACTCTCACCATCACCAGC19400.10765355753389007No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19390.10759806600938807No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19370.10748708296038403No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18990.10537840502930784No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18340.10177145593667752No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18160.10077260849564142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)