FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756758
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATGAGCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGG80000.4553842931126541No Hit
CATTATTACTGTGCAGCATGGGATGACAGCCTGCATGGTTGGGTGTTCGG78490.44678891458015274No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC60280.3431320648603849No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC54390.3096043962799657No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTGGGTCCCTCCGCCGAACACCCAACCATGCAGGCTGTCATCCCATG52080.2964551748163378No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46510.2647490434083693No Hit
CATCTATAGTGATAATCAGCGGCCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCGGCCTC45690.26008135440396457No Hit
ACCACATACTATGCAAAGTCTGTGAAGGGCAGATTCATCATCTCCAGAGACAATTCCAGGAACACTCTGGATCTT43710.24881059314942636No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41940.23873521566430891No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT39570.22524445598084653No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTGGGTCCCTC38760.2206336900130809No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG36250.2063460078166714No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35360.20127985755579314No Hit
GACCTAGGACGGTCAACTGGGTCCCTCCGCCGAACACCCAACCATGCAGG35310.20099524237259772No Hit
CTATAGATGAGGGGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTAACACCATTACTTCGGATGTTTAAG35020.19934447431006436No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGGAGAGGGTCAC34820.19820601357728268No Hit
GTATGTGGTACCCCCATTACTAACAATTCCTGAAACATATTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTG32900.18727679054257898No Hit
CAATAATACACAGCCATGTCGTCATGTCTCAGGCTGCCCAGTTGAAGATC32570.1853983303334893No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32520.1851137151502939No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32100.18272294761145247No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32000.18215371724506163No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA31810.1810721795489191No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31140.1772583360941006No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC30870.1757214141048454No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30780.17520910677509366No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG30560.17395679996903388No Hit
CCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCGGCCTCCCTGGCCATCAGTGGACTCCAGT29720.169175264891351No Hit
GTATTATTGTGCGAGAGGGGAAGGGAAGTGGTTATGGCGCTACTTTGACCACTGGGGCCAGGGAACCCTGGTCAC29370.16718295860898313No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC29340.1670121894990659No Hit
ATAATACACAGCCATGTCGTCATGTCTCAGGCTGCCCAGTTGAAGATCCA29200.16621526698611874No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29010.1651337292899762No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28890.16445065285030722No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28630.16297065389769108No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28490.16217373138474395No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28370.16149065494507495No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27900.15881527222303812No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26700.15198450782634831No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT26680.15187066175307012No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCGGTGACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGG26380.1501629706538977No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG26360.15004912458061953No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA25760.14663374238227464No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT25680.14617835808916196No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25670.14612143505252287No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25500.1451537434296585No Hit
GTGTTAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACCCCTCATC25470.14498297431974125No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCAGTGGACTCCAGTCTGAGGATG24690.14054297746189287No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGCATGGTTGGGTGTTCGGCG24670.1404291313886147No Hit
CCCTCAGCGTCTGGGACCCCCGGGGAGAGGGTCACCATCTCTTGTTCAGG24430.13906297850927674No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23860.13581836542084907No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23400.13319990573545132No Hit
GCATGGGATGACAGCCTGCATGGTTGGGTGTTCGGCGGAGGGACCCAGTTGACCGTCCTAGGTCAGCCCAAGG23170.13189067589275244No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC23070.13132144552636163No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22960.13069529212333172No Hit
GGATGACAGCCTGCATGGTTGGGTGTTCGGCGGAGGGACCCAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC22890.13029683086685817No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC22810.1298414465737455No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22700.12921529317071562No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22570.12847529369440755No Hit
CTCCTGAGGGCCGCTGATTATCACTATAGATGAGGGGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTAA22360.12727990992498683No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21970.12505991149606263No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21790.12403529683655916No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21520.12249837484730397No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21200.12067683767485334No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21180.12056299160157517No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21140.12033529945501885No Hit
GTGATAATCAGCGGCCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAG21130.12027837641837977No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21030.11970914605198896No Hit
ACCTAGGACGGTCAACTGGGTCCCTCCGCCGAACACCCAACCATGCAGGC20570.11709068636659117No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20410.11617991778036588No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC20120.11452914971783251No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19870.11310607380185546No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19690.11208145914235199No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACTCCTGAGGGCCGCTG19620.11168299788587842No Hit
GTGTATTATTGTGCGAGAGGGGAAGGGAAGTGGTTATGGCGCTACTTTGA19590.11151222877596118No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG19560.11134145966604392No Hit
GATTATCACTATAGATGAGGGGTTTGGGGGCCGTTCCTGGGAGCTGCTGG19490.11094299840957035No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC19350.11014607589662322No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT19330.11003222982334504No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT19220.10940607642031515No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19140.10895069212720249No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18930.10775530835778178No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTGGGTCCCTCCGCCGAACAC18910.10764146228450361No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18870.10741377013794728No Hit
CTCTTGTTCAGGAAGCGGCTTAAACATCCGAAGTAATGGTGTTAACTGGTACCAGCAGCTCCCAGGAACGGCCCC18860.1073568471013082No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18640.10610454029524842No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18610.10593377118533116No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGGAGAGG18220.10371377275640697No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG18190.10354300364648972No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC18180.10348608060985064No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG18130.10320146542665523No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17890.10183531254731727No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAATATGTTTC17810.10137992825420464No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17720.10086762092445288No Hit
ATCCCATGCTGCACAGTAATAATGAGCCTCATCCTCAGACTGGAGTCCAC17700.10075377485117472No Hit
GCCTGAGACATGACGACATGGCTGTGTATTATTGTGCGAGAGGGGAAGGG17700.10075377485117472No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT17590.10012762144814481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)