FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756758
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATGAGCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGG74890.42629662139008334No Hit
CATTATTACTGTGCAGCATGGGATGACAGCCTGCATGGTTGGGTGTTCGG73830.4202627795063406No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC57390.3266813072716902No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC54790.31188131774552896No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51860.295202868010278No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTGGGTCCCTCCGCCGAACACCCAACCATGCAGGCTGTCATCCCATG51340.29224287010504574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48410.2755644203697948No Hit
CATCTATAGTGATAATCAGCGGCCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCGGCCTC42340.24101213712987216No Hit
ACCACATACTATGCAAAGTCTGTGAAGGGCAGATTCATCATCTCCAGAGACAATTCCAGGAACACTCTGGATCTT40320.22951368372877765No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT38240.21767369210784868No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37680.21448600205606008No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35890.20429677849766445No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTGGGTCCCTC35260.20071062718940233No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGGAGAGGGTCAC34960.19900293609022984No Hit
CTATAGATGAGGGGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTAACACCATTACTTCGGATGTTTAAG33890.1929121711698481No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT33550.19097678792411932No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33140.18864294342191695No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32090.18266602457481337No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31980.1820398711717835No Hit
GTATGTGGTACCCCCATTACTAACAATTCCTGAAACATATTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTG31340.17839679682688225No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30880.17577833714148447No Hit
GACCTAGGACGGTCAACTGGGTCCCTCCGCCGAACACCCAACCATGCAGG30290.17241987797977865No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30020.17088295599052347No Hit
GTATTATTGTGCGAGAGGGGAAGGGAAGTGGTTATGGCGCTACTTTGACCACTGGGGCCAGGGAACCCTGGTCAC29740.16928911096462915No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29580.16837834237840385No Hit
CAATAATACACAGCCATGTCGTCATGTCTCAGGCTGCCCAGTTGAAGATC29180.16610142091284058No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28900.16450757588694628No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28330.16126296279851865No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28310.16114911672524046No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27840.15847373400320364No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG27460.1563106586109185No Hit
ATAATACACAGCCATGTCGTCATGTCTCAGGCTGCCCAGTTGAAGATCCA27450.15625373557427943No Hit
GTGTTAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACCCCTCATC27150.154546044475107No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26980.15357835285224258No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26260.1494798942142287No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26200.1491383559943942No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25880.1473168188219436No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCGGTGACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGG25760.14663374238227464No Hit
CCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCGGCCTCCCTGGCCATCAGTGGACTCCAGT25760.14663374238227464No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25410.14464143609990676No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25370.14441374395335044No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25340.14424297484343318No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25260.14378759055032053No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCAGTGGACTCCAGTCTGAGGATG25190.14338912929384695No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24960.14207989945114807No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT24460.139233747619194No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTGGGTCCCTCCGCCGAACAC24220.13786759473985602No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGCATGGTTGGGTGTTCGGCG24220.13786759473985602No Hit
GGATGACAGCCTGCATGGTTGGGTGTTCGGCGGAGGGACCCAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC23710.13496451987126287No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC23590.13428144343159387No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23290.1325737523324214No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC23160.13183375285611337No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23030.1310937533798053No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22540.1283045245844903No Hit
CCCTCAGCGTCTGGGACCCCCGGGGAGAGGGTCACCATCTCTTGTTCAGG22480.1279629863646558No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22430.1276783711814604No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22090.1257429879357316No Hit
CTCCTGAGGGCCGCTGATTATCACTATAGATGAGGGGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTAA22050.1255152957891753No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21570.12278299003049938No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21560.12272606699386028No Hit
GCATGGGATGACAGCCTGCATGGTTGGGTGTTCGGCGGAGGGACCCAGTTGACCGTCCTAGGTCAGCCCAAGG21530.12255529788394302No Hit
GTGATAATCAGCGGCCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAG21110.12016453034510159No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20900.11896914657568089No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20600.11726145547650843No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20400.1161229947437268No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG20280.11543991830405781No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACTCCTGAGGGCCGCTG20190.11492761097430608No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20110.11447222668119342No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19950.11356145809496812No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19340.11008915285998414No Hit
CTCTTGTTCAGGAAGCGGCTTAAACATCCGAAGTAATGGTGTTAACTGGTACCAGCAGCTCCCAGGAACGGCCCC19320.10997530678670596No Hit
GATTATCACTATAGATGAGGGGTTTGGGGGCCGTTCCTGGGAGCTGCTGG19070.1085522308707289No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC18950.10786915443105993No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT18950.10786915443105993No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18730.10661684762500014No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA18660.10621838636852657No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18600.10587684814869207No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC18590.10581992511205299No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18290.10411223401288053No Hit
GTGTATTATTGTGCGAGAGGGGAAGGGAAGTGGTTATGGCGCTACTTTGA18240.10382761882968512No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC18130.10320146542665523No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18110.10308761935337708No Hit
ACCTAGGACGGTCAACTGGGTCCCTCCGCCGAACACCCAACCATGCAGGC18070.10285992720682074No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18010.10251838898698626No Hit
GGGTTCCCTGGCCCCAGTGGTCAAAGTAGCGCCATAACCACTTCCCTTCC18000.10246146595034716No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17910.10194915862059545No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17820.10143685129084369No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTGGGTC17700.10075377485117472No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC17600.10018454448478391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA300.00227381869.6156770
ATTCCGT200.00782288650.95714621
GGTATCA15100.046.380781
GTGGTAT4250.045.5967331
TGGTATC4750.040.0585142
GTATCAA34200.035.389281
GACAGGT2400.032.5811
TATCAAC39350.030.5674292
GGTACGG450.004990846430.190813
TCAACGC41250.029.476374
ATCAACG41250.029.312533
CAACGCA41700.029.1582785
AACGCAG42300.028.6652096
GCCAGAG33100.028.39309570
ACTAGAC500.007426768527.8316546
CGCAGAG44500.027.4007038
ACGCAGA44450.027.3551147
GTCCTAG39750.027.32086470
CACTCGG650.00893890726.7938269
GCAGAGT45850.026.5931649
Produced by FastQC (version 0.11.5)