FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159839
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCATCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGA47220.40712547172495495No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33300.2871088142405972No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32930.28391871630459053No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATACTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26750.23063545888696624No Hit
GTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT25740.22192735371029942No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTAGATTCACTTTCAGTAAGGCCTCG25060.21606447101709808No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25030.21580581442769212No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA24900.21468496920693303No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21510.1854567746040614No Hit
GTGTATTACTGTCATCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAA20950.1806285182684838No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20030.17269638286003489No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC19690.1697649415134342No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA18980.16364340223082688No Hit
GTCTGGGACAGACTTCACTCTCAGTATCAGCAGACTGGAGCCTGAAGATT18540.15984977225287303No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18480.15933245907406116No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18450.15907380248465522No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18380.158470270442708No Hit
GTATTACTGTACCACAGTCCTTCTTCTATGGTTCGCGAACTCTATCGGCT18310.1578667384007608No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18170.1566596743168664No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17900.1543317650122129No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCTTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG16820.14502012779359894No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16790.144761471204193No Hit
CAGTAATACAGGGCTGTGTCCTCGGTTTTCAGGCTGGTCATTTGCAGATA16730.14424415802538113No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16480.14208868644699824No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16290.14045052804742725No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15940.13743286783769126No Hit
GGACTGAACCCAGAGGACTCACCATGGAATTTGGGCTGAGCTGGATTTTC15890.1370017735220147No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCTTCTCCTGCAGGGCCAGTCAGA14570.12562088358815318No Hit
GTAATACAGGGCTGTGTCCTCGGTTTTCAGGCTGGTCATTTGCAGATATA14560.12553466472501787No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14490.12493113268307067No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14450.1245862572305294No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCTTCTCCTGC14160.12208591019960528No Hit
CTCCTGTGCAGCCTCTAGATTCACTTTCAGTAAGGCCTCGATGACCTGGGTCCGCCAGACTCCAGGGAAGGGG14150.12199969133646997No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14110.12165481588392872No Hit
CCATAGAAGAAGGACTGTGGTACAGTAATACAGGGCTGTGTCCTCGGTTTTCAGGCTGGTCATTTGCAGATATAG14090.12148237815765808No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT14030.1209650649788462No Hit
GGCCAAAAGTGTACGGTGAGCTACCATACTGATGACAGTAATACACTGCA13590.11717143500089236No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATACTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13550.11682655954835111No Hit
ATACAGGGCTGTGTCCTCGGTTTTCAGGCTGGTCATTTGCAGATATAGCG13390.11544705773818607No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13330.11492974455937419No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13200.11380889933861511No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13080.11277427298099132No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCC12650.10906686186617282No Hit
CTACCATACTGATGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATACTGAGAGTGAAGTCTGTC12600.10863576755049623No Hit
GATCAGGACTGAACCCAGAGGACTCACCATGGAATTTGGGCTGAGCTGGA12330.10630785824584275No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12090.10423860553059519No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11980.10329019803610674No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11900.10260044713102422No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11880.10242800940475358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAAA350.00630250253.8155470
GGTATCA13900.042.9051551
TCGTATG1700.036.5955840
CGTATGC1750.035.58094441
ATCTCGT1650.033.42936737
GTATCAA30100.033.363851
ATGCGCA2000.030.88995729
CGAGACA2150.030.29328723
ACGAGAC2050.030.09755122
ATGCCGT2100.029.73621444
CTTATAC2100.029.3784081
GTATGCC2250.029.23441942
TATCAAC34700.028.6397112
ATCAACG34750.028.499893
GTGGTAT4700.028.4407981
ACATGCG2200.028.06723427
TATACAC2450.027.9746113
TCAACGC35450.027.9371284
TGGTATC4800.027.843482
TGCCGTC2250.027.78290445
Produced by FastQC (version 0.11.5)