FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159839
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCATCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGA44450.38324284663647284No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33780.2912473196710923No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27980.2412403790526099No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25080.2162369087433687No Hit
GTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT24240.2089945242400023No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATACTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23940.20640795834594283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23450.20218323405231242No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTAGATTCACTTTCAGTAAGGCCTCG23080.1989931361163058No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA22280.19209562706548064No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20420.17605891852231215No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20410.17597269965917683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20210.17424832239647053No Hit
GTGTATTACTGTCATCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAA19640.16933384719775763No Hit
GTCTGGGACAGACTTCACTCTCAGTATCAGCAGACTGGAGCCTGAAGATT19350.1668335001668335No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18860.1626087758732031No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18590.1602808665685496No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18270.15752186294821952No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA17600.15174519911815346No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC17270.1488999766346881No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16400.14139893554191574No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16340.14088162236310384No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCTTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG16010.13803639987963848No Hit
GTATTACTGTACCACAGTCCTTCTTCTATGGTTCGCGAACTCTATCGGCT15980.1377777432902325No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCTTCTCCTGCAGGGCCAGTCAGA15650.13493252080676715No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15330.1321735171864371No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15160.13070779651313674No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14810.12769013630340073No Hit
GGACTGAACCCAGAGGACTCACCATGGAATTTGGGCTGAGCTGGATTTTC14550.12544844586188256No Hit
CTCCTGTGCAGCCTCTAGATTCACTTTCAGTAAGGCCTCGATGACCTGGGTCCGCCAGACTCCAGGGAAGGGG14530.12527600813561193No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14430.12441381950425878No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14170.12217212906274061No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14160.12208591019960528No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14160.12208591019960528No Hit
CAGTAATACAGGGCTGTGTCCTCGGTTTTCAGGCTGGTCATTTGCAGATA14040.12105128384198151No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCTTCTCCTGC13540.11674034068521578No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATACTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13290.11458486910683294No Hit
GTAATACAGGGCTGTGTCCTCGGTTTTCAGGCTGGTCATTTGCAGATATA13200.11380889933861511No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13140.1132915861598032No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13050.11251561639158537No Hit
GGCCAAAAGTGTACGGTGAGCTACCATACTGATGACAGTAATACACTGCA12910.11130855230769099No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12660.10915308072930811No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT12520.10794601664541371No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCC12450.10734248460346651No Hit
CTACCATACTGATGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATACTGAGAGTGAAGTCTGTC11920.10277288485729486No Hit
GATCAGGACTGAACCCAGAGGACTCACCATGGAATTTGGGCTGAGCTGGA11700.10087606986831793No Hit
CCATAGAAGAAGGACTGTGGTACAGTAATACAGGGCTGTGTCCTCGGTTTTCAGGCTGGTCATTTGCAGATATAG11680.10070363214204729No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGT200.007551142551.413829
GGTATCA13350.045.3424641
GTATCAA29700.035.5807531
GTGGTAT3300.034.198981
AACGAAC1253.6379788E-1233.59180551
TCCGAGA7300.031.62797270
ACGAACC1357.2759576E-1231.13100252
TGGTATC3750.031.001722
TATCAAC33900.030.8645482
ATCAACG34350.030.5531643
TCAACGC34850.030.0193064
CAAACGA1401.2732926E-1129.9371849
CAACGCA35350.029.5908765
AACGCAG35500.029.5634126
CGAACCT1451.8189894E-1129.02378553
ATAGCGG1352.1282176E-1029.01095663
ACAAACG1452.0008883E-1128.87306448
ACGGGAT1103.98868E-827.98098216
CGCAGAG37850.027.726718
ACGCAGA38100.027.5459637
Produced by FastQC (version 0.11.5)