FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076392
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATGGTGGCCTCCGCTCACTTTCGGGGGGGGGACCAAGGTGGAAATCAGAGGAACTGTGGCTGCACCAT57420.5334487807415885No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45290.4207574935525348No Hit
CCATTTATACTGTTGACAGAAATAGACTGCAGAATCCTCAGACTGCAGGCTGGTTATGGTGAGAGAGAACT44050.40923752684895465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43130.4006904547785566No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32890.3055578265167337No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACTG30260.2811243487502694No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28580.2655166519260641No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC26870.24963024622999802No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26200.24340574809177326No Hit
GTCTGTGTCTCCGGGGGAAAGAGCCACCCTCGCGTGCAGGGCCAGTCAGAATGTAGAAAATATGGTAGGATGGTA26190.2433128451344863No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25210.23420835532036657No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25090.23309351983292334No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24800.23039933407160215No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24490.22751934239570712No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23140.2149774431619707No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCGGGGGAAAGAGCCACCCTCGCGT22670.21061100416948472No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22550.2094961686820415No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22050.20485102081769466No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21620.2008561936543564No Hit
GTCCGGGACAGAGTTCTCTCTCACCATAACCAGCCTGCAGTCTGAGGATT20270.18831429442062No Hit
GAGTTCTCTCTCACCATAACCAGCCTGCAGTCTGAGGATTCTGCAGTCTATTTCTGTCAACAGTATAAATGGTGG20000.18580591457387272No Hit
GTCTATTTCTGTCAACAGTATAAATGGTGGCCTCCGCTCACTTTCGGGGG19270.17902399869192637No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19000.17651561884517908No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18880.17540078335773585No Hit
GTAATAGACGGCCGTGTCAGCGGCAGTGACAGAGTCCAGGTGCAGGGAGA18560.1724278887245539No Hit
GAAATAGACTGCAGAATCCTCAGACTGCAGGCTGGTTATGGTGAGAGAGAACTCTGTCCCGGACCCACTGCCG17600.16350920482500797No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17570.16323049595314718No Hit
CACCATAACCAGCCTGCAGTCTGAGGATTCTGCAGTCTATTTCTGTCAACAGTATAAATGGTGGCCTCCGCTCA16710.15524084162647067No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16660.15477632684003595No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16610.15431181205360128No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15740.14622925476963783No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15550.14446409858118603No Hit
CTTTCGGGGGGGGGACCAAGGTGGAAATCAGAGGAACTGTGGCTGCACCA15410.14316345717916892No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14930.138704115229396No Hit
GGCCTCTGGTGTCTCCGCCAGGTTCAGCGGCAGTGGGTCCGGGACAGAGT14720.13675315312637032No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14520.1348950939806316No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14210.13201510230473656No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14030.13034284907357171No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13780.1280202751413983No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13770.12792737218411135No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13740.12764866331225055No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13620.12653382782480732No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13590.1262551189529465No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13590.1262551189529465No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13360.12411835093534697No Hit
GTCTATTACTGTGCCAGAGATGTGGGTGTGTCCTACTCTATTAATGGGGC13340.1239325450207731No Hit
GCAGAAACCTGGCCAGGCTCCCAAACTCCTCATCTATGCTGCCTCTACTCGGGCCTCTGGTGTCTCCGCCAGGTT13340.1239325450207731No Hit
CATCTATGCTGCCTCTACTCGGGCCTCTGGTGTCTCCGCCAGGTTCAGCGGCAGTGGGTCCGGGACAGAGTTCT13330.12383964206348617No Hit
CTGCTGGAGATGTGGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCT12990.12068094151573033No Hit
CCCAGAGACTGCTGGAGATGTGGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCGGGGGAAAGAGCCAC12770.11863707645541773No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTCTGATTTCCACCTTGGTCCCCCCCCCGAAAGTGAGCGGAGGCCA12760.1185441734981308No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12720.11817256166898304No Hit
CCCCGAAAGTGAGCGGAGGCCACCATTTATACTGTTGACAGAAATAGACT12650.11752224096797448No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12610.11715062913882676No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12540.1165003084378182No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12540.1165003084378182No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12500.11612869660867044No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12420.11538547295037495No Hit
CTGCTACTCTGGCTCCCAGAGACTGCTGGAGATGTGGTGATGACGCAGTC12250.11380612267649703No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12060.11204096648804525No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12030.11176225761618444No Hit
CCAGAGGCCCGAGTAGAGGCAGCATAGATGAGGAGTTTGGGAGCCTGGCC12010.11157645170161057No Hit
GGGCCAGTCAGAATGTAGAAAATATGGTAGGATGGTACCAGCAGAAACCT11920.11074032508602814No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11910.11064742212874121No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11910.11064742212874121No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11840.10999710142773264No Hit
CCCTGTCTGTGTCTCCGGGGGAAAGAGCCACCCTCGCGTGCAGGGCCAGT11790.10953258664129796No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11740.10906807185486328No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11560.10739581862369843No Hit
CCATAACCAGCCTGCAGTCTGAGGATTCTGCAGTCTATTTCTGTCAACAG11500.10683840087997681No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11360.10553775947795971No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11290.10488743877695116No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11280.1047945358196642No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11210.10414421511865567No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11100.10312228258849934No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11030.1024719618874908No Hit
GCCTCTACTCGGGCCTCTGGTGTCTCCGCCAGGTTCAGCGGCAGTGGGTC11000.10219325301562998No Hit
CTCCTCATCTATGCTGCCTCTACTCGGGCCTCTGGTGTCTCCGCCAGGTT10950.1017287382291953No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10900.10126422344276062No Hit
GTCTGAGGATTCTGCAGTCTATTTCTGTCAACAGTATAAATGGTGGCCTC10860.10089261161361288No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10820.10052099978446515No Hit
AGGTCACACTGAGTGACTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10790.10024229091260434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTAA200.007595674651.3371419
GGTATCA9750.047.034921
GTGGTAT2350.040.7765431
CGTGGTA350.001198891140.3784860
CACCGAA6050.039.64574470
TGGTATC2450.039.1085622
GTATCAA28000.038.2566151
CGAGACA2600.038.18441823
GAGCGAT2650.036.2935432
CGTATGC2050.035.4298941
TATACAC3100.035.323863
TACACAT3200.035.2893685
TTCGTGC400.002671468334.2996630
ACGAGAC2900.034.23430322
CTTATAC3100.034.223171
TCTCCGA2900.034.21999411
TCGTATG1950.033.67103640
ATACACA3200.033.1506164
TATCAAC31950.033.095392
GTACGTA652.463189E-432.89269
Produced by FastQC (version 0.11.5)