FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076392
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATGGTGGCCTCCGCTCACTTTCGGGGGGGGGACCAAGGTGGAAATCAGAGGAACTGTGGCTGCACCAT53850.5002824249901523No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44700.41527621907260553No Hit
CCATTTATACTGTTGACAGAAATAGACTGCAGAATCCTCAGACTGCAGGCTGGTTATGGTGAGAGAGAACT42520.39502337438405344No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36020.3346364521475448No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32930.30592943834588143No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27520.25566893845364885No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACTG27110.2518599172048845No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26640.24749347821239848No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26230.24368445696363408No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25180.23392964644850575No Hit
GTCTGTGTCTCCGGGGGAAAGAGCCACCCTCGCGTGCAGGGCCAGTCAGAATGTAGAAAATATGGTAGGATGGTA23920.22222387383035178No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22740.21126132487049326No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22590.20986778051118923No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22580.2097748775539023No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC21920.20364328237296453No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21510.1998342611242001No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21340.19825491085032218No Hit
GAGTTCTCTCTCACCATAACCAGCCTGCAGTCTGAGGATTCTGCAGTCTATTTCTGTCAACAGTATAAATGGTGG19800.183947855428134No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19480.180974960794952No Hit
GTCCGGGACAGAGTTCTCTCTCACCATAACCAGCCTGCAGTCTGAGGATT19360.1798601253075088No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18830.17493626857130115No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCGGGGGAAAGAGCCACCCTCGCGT18470.17159176210897145No Hit
GTCTATTTCTGTCAACAGTATAAATGGTGGCCTCCGCTCACTTTCGGGGG18410.17103434436524984No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18220.16926918817679806No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18050.16768983790292014No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17470.16230146638027781No Hit
GTAATAGACGGCCGTGTCAGCGGCAGTGACAGAGTCCAGGTGCAGGGAGA17410.1617440486365562No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17330.1610008249782607No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16640.1545905209254621No Hit
GAAATAGACTGCAGAATCCTCAGACTGCAGGCTGGTTATGGTGAGAGAGAACTCTGTCCCGGACCCACTGCCG16640.1545905209254621No Hit
CACCATAACCAGCCTGCAGTCTGAGGATTCTGCAGTCTATTTCTGTCAACAGTATAAATGGTGGCCTCCGCTCA16000.14864473165909817No Hit
CATCTATGCTGCCTCTACTCGGGCCTCTGGTGTCTCCGCCAGGTTCAGCGGCAGTGGGTCCGGGACAGAGTTCT15080.14009765958870002No Hit
GGCCTCTGGTGTCTCCGCCAGGTTCAGCGGCAGTGGGTCCGGGACAGAGT14430.13405896736504916No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14420.13396606440776224No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14420.13396606440776224No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14120.13117897568915415No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13980.12987833428713705No Hit
GTCTATTACTGTGCCAGAGATGTGGGTGTGTCCTACTCTATTAATGGGGC13650.12681253669666814No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13640.1267196337393812No Hit
GTCCCGGACCCACTGCCGCTGAACCTGGCGGAGACACCAGAGGCCCGAGT13550.12588350712379875No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13460.12504738050821634No Hit
CTCCTCATCTATGCTGCCTCTACTCGGGCCTCTGGTGTCTCCGCCAGGTT13150.12216738883232131No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13110.12179577700317357No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13040.12114545630216501No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12820.1191015912418524No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12470.11584998773680964No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12440.11557127886494883No Hit
CTTTCGGGGGGGGGACCAAGGTGGAAATCAGAGGAACTGTGGCTGCACCA12230.11362031676192316No Hit
CCCCGAAAGTGAGCGGAGGCCACCATTTATACTGTTGACAGAAATAGACT12130.1126912871890538No Hit
CTGCTGGAGATGTGGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCT12120.11259838423176687No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12070.11213386944533218No Hit
GCAGAAACCTGGCCAGGCTCCCAAACTCCTCATCTATGCTGCCTCTACTCGGGCCTCTGGTGTCTCCGCCAGGTT12050.11194806353075831No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11980.11129774282974975No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11840.10999710142773264No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTCTGATTTCCACCTTGGTCCCCCCCCCGAAAGTGAGCGGAGGCCA11830.10990419847044572No Hit
GTCTGAGGATTCTGCAGTCTATTTCTGTCAACAGTATAAATGGTGGCCTC11820.10981129551315878No Hit
GGGCCAGTCAGAATGTAGAAAATATGGTAGGATGGTACCAGCAGAAACCT11750.10916097481215022No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11750.10916097481215022No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11750.10916097481215022No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11630.108046139324707No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11620.10795323636742006No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11460.10646678905082907No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11420.10609517722168131No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11340.10535195356338584No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11110.1032151855457863No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11030.1024719618874908No Hit
CTGCTACTCTGGCTCCCAGAGACTGCTGGAGATGTGGTGATGACGCAGTC11020.10237905893020387No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10980.10200744710105612No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10910.10135712640004756No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10910.10135712640004756No Hit
GATGAGGAGTTTGGGAGCCTGGCCAGGTTTCTGCTGGTACCATCCTACCATATTTTCTACATTCTGACTGGCC10780.1001493879553174No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10780.1001493879553174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT702.4734818E-548.57988770
GTGGTAT2700.048.0781561
GGTATCA9750.043.7958951
ACGCACG503.7342197E-641.5725542
TGGTATC3250.039.9325752
GTATCAA25100.037.5632671
CACCGAA6850.036.7363270
CCGCTAT800.002785306234.0059270
GACAGGT2300.032.6755451
TATCAAC29250.032.2262882
ATCAACG29650.031.440133
TCAACGC29900.031.17874
GCGAATG702.8208038E-530.95061766
TGCGAAT703.0216408E-530.64055465
CAACGCA30650.030.5271785
AACGCAG30900.030.2801936
GCTTGTA802.9824769E-629.8906971
CGCACGG703.71778E-529.72409843
TACGCAC703.768345E-529.6653241
GTACGTA755.181426E-428.97292369
Produced by FastQC (version 0.11.5)