FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80380
Sequences flagged as poor quality0
Sequence length20-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT30873.840507588952476No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC5730.7128638964916646No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGT3970.493903956208012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.4628016919631749No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC3610.44911669569544665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.42547897486937053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.42299079372978354No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT2690.33466036327444637No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGG2480.3085344613087833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.2985817367504354No Hit
CCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC2400.2985817367504354No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG2400.2985817367504354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.2911171933316746No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.28489674048270713No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.2662353819358049No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGG2110.2625031102264245No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTC2010.25006220452848965No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.24632993281910923No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.2289126648420005No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.22766857427220702No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT1800.2239363025628266No Hit
GTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTA1780.2214481214232396No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.21771584971385918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.2139835780044787No Hit
GAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTT1690.21025130629509828No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCGTACTCTGCGTTGATAC1650.20527494401592436No Hit
GTATCAACGCAGAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGG1640.20403085344613087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1580.19656631002737No Hit
GTATCAACGCAGAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT1560.19407812888778303No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT1520.1891017666086091TruSeq Adapter, Index 1 (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.18163722318984823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.17914904205026125No Hit
GGTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT1430.17790495148046778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.1766608609106743No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATACC1390.17292858920129384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.17168449863150037No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.17044040806170688No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.16546404578253296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.1642199552127395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.16173177407315253No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.15799950236377208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.15675541179397862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.15302314008459816No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.15177904951480467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1200.1492908683752177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.1455585966658373No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.1455585966658373No Hit
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC1150.1430704155262503No Hit
GGTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAG1150.1430704155262503No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGG1120.13933814381686987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.13436178153769593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.13436178153769593No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGTCGTATGCCGT1050.1306295098283155No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.1256531475491416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.1256531475491416No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT1000.1244090569793481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT990.12316496640955461No Hit
CCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATACC990.12316496640955461No Hit
CCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC990.12316496640955461No Hit
TATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGG970.12067678526996765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG960.11943269470017417No Hit
GTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAG940.11694451356058722No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCGTACTC920.11445633242100026No Hit
GGTATCAACGCAGAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT900.1119681512814133No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.1107240607116198No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.10947997014182634No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG870.10823587957203284No Hit
CCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCGTACTCTGCGTTGATAC870.10823587957203284No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTAC860.10699178900223935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT850.10574769843244589No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT840.10450360786265239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.10325951729285893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG810.10077133615327195No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATGA100.0023739364208.5496270
GTATATT100.0023739364208.5496270
ATCGGAT100.006401618149.7930568
AGGGGGG3250.0144.3805170
CATCGGA100.007491797142.1303667
CCTACCA150.007983496139.033170
GTGTATT150.007983496139.033170
GCATTGG150.007983496139.033170
CGGGCCT100.008375776136.9332366
AAGACCA100.008375776136.9332366
TGTACCG100.009156204132.918265
AAAGACC100.009156204132.918265
TTTAAGG100.009805353129.9110164
AAGGGGG3250.0114.7914369
TGTGTGT304.5056152E-4104.2748270
GCTTCTG157.0314726E-493.4175750
AAAGGGG3800.088.69324568
ATGGGGG850.085.8733869
GCCGGCT150.001092604783.6223346
AAAAGGG4100.079.7316767
Produced by FastQC (version 0.11.5)