FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80380
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62057.719581985568549No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.5685493903956208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.5411793978601642No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGT3610.44911669569544665No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT3480.4329435182881314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.4043294351828813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.33714854441403336No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC2710.33714854441403336No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.328439910425479No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.31599900472754416No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGG2400.2985817367504354No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.28987310276188105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2310.2873849216222941No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.27245583478477237No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGG2170.2699676536451854No Hit
CCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC2090.26001492908683754No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.2488181139586962No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT1970.24508584224931573No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.24384175167952224No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.2363772082607614No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.2363772082607614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.2239363025628266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.2226922119930331No Hit
GTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTA1760.21895994028365268No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTC1750.21771584971385918No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.2139835780044787No Hit
GAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTT1710.21273948743468526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.2077631251555113No Hit
GTATCAACGCAGAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGG1660.20651903458571785No Hit
GTATCAACGCAGAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT1660.20651903458571785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.20527494401592436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1610.20029858173675044No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCGTACTCTGCGTTGATAC1600.19905449116695695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.18661358546902215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1480.1841254043294352No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.1828813137596417No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATACC1460.18163722318984823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.18039313262005474No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1410.17541677034088082No Hit
GGGTACATGGCGTTAGGTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.17292858920129384No Hit
GGTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT1370.17044040806170688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1370.17044040806170688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.1691963174919134No Hit
GGTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAG1340.16670813635232645No Hit
CCCGAAAGGGTACATGGCGTTAGGTCGCAAAAAAAAAAAAAAAAAAAAAA1330.16546404578253296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.16546404578253296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.162975864642946No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.16173177407315253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.15177904951480467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1130.14058223438666334No Hit
GCGTTAGGTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.13560587210748942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT1090.13560587210748942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1070.13311769096790246No Hit
GTACATGGCGTTAGGTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.13311769096790246No Hit
CCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC1060.13187360039810897No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.12814132868872855No Hit
TATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGG1000.1244090569793481No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.1244090569793481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.12316496640955461No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.12316496640955461No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGG970.12067678526996765No Hit
GGTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGG970.12067678526996765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.11694451356058722No Hit
GAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT940.11694451356058722No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT930.11570042299079372No Hit
GGTACATGGCGTTAGGTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.11445633242100026No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.11445633242100026No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTT920.11445633242100026No Hit
AAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGTCGCAAAAAAA910.11321224185120676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG910.11321224185120676No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG890.1107240607116198No Hit
GTATCAACGCAGAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGG890.1107240607116198No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCGTACTCTGCGTTGATACC890.1107240607116198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT880.10947997014182634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.10947997014182634No Hit
AAAGGGTACATGGCGTTAGGTCGCAAAAAAAAAAAAAAAAAAAAAAAAAA880.10947997014182634No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.10947997014182634No Hit
GTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAG870.10823587957203284No Hit
GTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT860.10699178900223935No Hit
CCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCGTACTCTGCGTTGATAC840.10450360786265239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG830.10325951729285893No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTAC820.10201542672306543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)