FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665069
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTATACTACTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGACATCAAAAGAACTGTGGCTGCACCAT93260.5600969088968686No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68320.41031332635464357No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64140.385209261598168No Hit
GTATAATGTTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC62500.3753598199233786No Hit
CTCCTAAGCAGCTCATTTACTGGGCATCTACCCGGGAATCAGGGGTCCCT55550.33361980794789886No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49070.294702501818243No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGTTAAGTAGTTCTTATTGTTGGTCGAATATAAAAGACT47020.28239069972475617No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT42880.2575268652530316No Hit
ATACTACTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGACATCAAAAGA42530.25542485026146067No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42500.2552446775478974No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA42480.2551245624055219No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42110.25290243227157555No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT40270.24185183917303127No Hit
GAGTAGTATAATGTTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT38840.23326360649318437No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37670.22623687066421874No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36830.2211920346844485No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36830.2211920346844485No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36320.21812909855387377No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35960.2159670259911151No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34600.20779919630958235No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33680.2022738997603102No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33230.1995713090568619No Hit
AACCAGGACAGCCTCCTAAGCAGCTCATTTACTGGGCATCTACCCGGGAA30350.18227472855479263No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28130.1689419477511142No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC27410.16461780262559692No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT26560.15951290907463894No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26300.1579514122237577No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCAGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT26280.15783129708138222No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAACATTATACTACT26010.15620974265931323No Hit
CAATAAGAACTACTTAACTTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGCAGCTCATTTACTGGGCATCTAC24940.14978358254222499No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24360.14630024341333603No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24310.1459999555573973No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATATTCGACCAA24000.14413817085057737No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23860.143297364853949No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23190.13927350758437038No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23110.13879304701486847No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22780.13681114716567302No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC22450.13482924731647755No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21620.1298444689078951No Hit
GTAAATGAGCTGCTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGTTA21440.1287634326265158No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21420.1286433174841403No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAACATTATACTACTCCTTTCACTTT21390.12846314477057708No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21300.1279226266298874No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21140.12696170549088356No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT21030.12630107220781842No Hit
GTCCTGGTTTCTGCTGATACCAAGTTAAGTAGTTCTTATTGTTGGTCGAA20680.12419905721624748No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20390.12245738765180302No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20290.1218568119399256No Hit
CTTTTATATTCGACCAACAATAAGAACTACTTAACTTGGTATCAGCAGAA20220.12143640894161144No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT19980.11999502723310565No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19970.11993496966191791No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19940.1197547969483547No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19710.11837347281103666No Hit
GTATTACTGTGTGAGACCCCAAAATTGCGGTGGTGACTGCTATTACTTTC19650.1180131273839102No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19640.11795306981272247No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19530.11729243652965733No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19440.11675191838896766No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC19270.11573093967877607No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTTTTGATGTCCACTTTGGTCC19080.114589845826209No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCC18990.11404932768551933No Hit
CTACTTAACTTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGCAGCTCA18930.1136889822583929No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18730.11248783083463809No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18590.11164702483800971No Hit
GTGCAGCCACAGTTCTTTTGATGTCCACTTTGGTCCCAGGGCCGAAAGTG18480.11098639155494457No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18480.11098639155494457No Hit
GTTTATTTCTGTCAGCAACATTATACTACTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGACATCAAAAGAA18410.11056598855663038No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18180.10918466441931236No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC18040.10834385842268399No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT18000.10810362813793303No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17840.10714270699892917No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17750.10660218885823951No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAACATTATACTACTCCTT17570.10552115257686018No Hit
CAGTAATACATGGCCGTGTCCTCAGATCTCAATCTGCTCAGCTCCATAAA17400.10450017386666859No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17400.10450017386666859No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17100.10269844673103637No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16810.10095677716659189No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC16710.1003562014547145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATG200.00819996350.353749
AATTCGT306.910991E-445.16168633
TATACAC7600.043.732193
TCGTATG7650.043.63945840
CGTATGC7800.042.9204941
GACGCGT7750.042.5304926
ATGCCGT7950.042.43428444
ACGCGTA7800.042.2909427
GCGTAGT7950.041.56183229
CGCGTAG7950.041.52801528
GCCGTCT8200.041.3489746
CGTAGTA8050.041.08891330
ATCTCGT7350.040.40595237
CGAGACG8150.040.38234723
CTTATAC8300.040.055961
GTAGTAA8300.039.8982831
CCGTCTT8450.039.80157547
AGACGCG8350.039.45422425
ACGAGAC8350.039.4103722
AATCTCG7500.039.04729536
Produced by FastQC (version 0.11.5)