FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665069
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTATACTACTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGACATCAAAAGAACTGTGGCTGCACCAT86450.5191977029180171No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79790.47919936050698203No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62230.37373826550130956No Hit
GTATAATGTTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC61760.3709155596554858No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60020.3604655422688189No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52480.3151821335932625No Hit
CTCCTAAGCAGCTCATTTACTGGGCATCTACCCGGGAATCAGGGGTCCCT49440.2969246319521894No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGTTAAGTAGTTCTTATTGTTGGTCGAATATAAAAGACT43850.2633524496582424No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43120.2589682469615373No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43030.25842772882084764No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40970.24605586915617308No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40270.24185183917303127No Hit
ATACTACTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGACATCAAAAGA39770.23884896061364425No Hit
GAGTAGTATAATGTTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT39740.23866878790008103No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT38480.2311015339304257No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36020.21632737141824154No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34020.2043158571806934No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33750.20269430275862443No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33180.19927102120092322No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33130.1989707333449845No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32090.1927247459414595No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29410.176629316863145No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT29000.17416695644444766No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26840.16119452106789567No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26050.15644997294406418No Hit
AACCAGGACAGCCTCCTAAGCAGCTCATTTACTGGGCATCTACCCGGGAA26010.15620974265931323No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAACATTATACTACT24540.14738127969471534No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24190.14527926470314442No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23880.14341747999632448No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCAGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT23860.143297364853949No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23770.14275684671325933No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23010.13819247130299103No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATATTCGACCAA22970.13795224101824008No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22930.13771201073348913No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22580.1356099957419182No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22240.13356803832153502No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21750.13062521733333574No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCTGATTCCCGGGT21330.12810279934345062No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21210.12738210848919773No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCT21150.1270217630620713No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20890.12546026621119005No Hit
CAATAAGAACTACTTAACTTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGCAGCTCATTTACTGGGCATCTAC20840.12515997835525133No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20780.1247996329281249No Hit
GTCCTGGTTTCTGCTGATACCAAGTTAAGTAGTTCTTATTGTTGGTCGAA20550.12341830879080687No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20450.12281773307892946No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20430.12269761793655398No Hit
GTAAATGAGCTGCTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGTTA20160.121076063514485No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19790.11885393338053858No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAACATTATACTACTCCTTTCACTTT19740.11855364552459988No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT19640.11795306981272247No Hit
GTGCAGCCACAGTTCTTTTGATGTCCACTTTGGTCCCAGGGCCGAAAGTG19390.11645163053302897No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19340.11615134267709025No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAACATTATACTACTCCTT19230.11549070939402512No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19060.11446973068383352No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18900.11350880954482967No Hit
GTATTACTGTGTGAGACCCCAAAATTGCGGTGGTGACTGCTATTACTTTC18860.11326857926007872No Hit
GTTTATTTCTGTCAGCAACATTATACTACTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGACATCAAAAGAA18850.11320852168889098No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18840.11314846411770324No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18660.1120674278363239No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC18620.11182719755157293No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18560.1114668521244465No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18500.11110650669732004No Hit
CAGTAATACATGGCCGTGTCCTCAGATCTCAATCTGCTCAGCTCCATAAA18490.1110464491261323No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT18440.11074616127019361No Hit
CTTTTATATTCGACCAACAATAAGAACTACTTAACTTGGTATCAGCAGAA18150.10900449170574912No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18130.10888437656337364No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18000.10810362813793303No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCC17930.10768322513961884No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17620.10582144043279888No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17560.10546109500567244No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17320.10401971329716668No Hit
GTTCTTTTGATGTCCACTTTGGTCCCAGGGCCGAAAGTGAAAGGAGTAGTATAATGTTGCTGACAGAAATAAA17160.10305879215816283No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17090.10263838915984863No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17040.10233810130390994No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16930.10167746802084478No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16830.10107689230896737No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16770.10071654688184094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)