FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554954
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25460.4587767634794956No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22890.4124666188548961No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAGGCCTCGTA21370.38507696133373215No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17760.3200265247209679No Hit
GTGCACACTGGCCCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA17230.31047618361161466No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14820.26704916083134816No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCCAG14810.2668689657160774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14000.25227316137914135No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13780.24830886884318343No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13570.2445247714224963No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13110.23623579612003878No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12930.2329922840451641No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12900.23245169869935164No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12670.2283072110481229No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC12590.2268656501259564No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12230.22037862597620705No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12170.21929745528458217No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT11770.21208965067374955No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11450.20632340698508345No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCC11260.20289969979493797No Hit
GTCTGGGACCCCAGGGTCCCGGTAAGAAACCCCATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGGTGAAA11100.2000165779506049No Hit
GATTTCACACTGAACATCAGCAGGGTGGAGGCTGAGGATGTTGGGATTTATTTCTGCATGCAAGGTGCACACTGG11080.1996561877200633No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11080.1996561877200633No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA10470.18866428568854354No Hit
AGTGTGCACCTTGCATGCAGAAATAAATCCCAACATCCTCAGCCTCCACC10380.1870425296511062No Hit
GTGTGCACCTTGCATGCAGAAATAAATCCCAACATCCTCAGCCTCCACCC10260.18488018826785643No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9940.17911394457919036No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9800.17659121296539892No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9450.1702843839309204No Hit
GAACATCAGCAGGGTGGAGGCTGAGGATGTTGGGATTTATTTCTGCATGCAAGGTGCACACTGGCCCCCCACTTT9430.16992399370037878No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9410.16956360346983715No Hit
CCTCTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG9060.1632567744353586No Hit
GTCCAAGGGTGACGGGCAGGGAGAGAGGAGACTGAGTCATCACAACATCC8940.16109443305210883No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG8780.1582113112077758No Hit
GCTAATGCTCTGGGTCCCAGCATCCAGTGGGGATGTTGTGATGACTCAGT8730.15731033563142172No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8640.15568857959398436No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATCCAGTGGGGATGTTGTGATGA8550.15406682355654705No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8460.1524450675191097No Hit
GTATACGAGGCCTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGAGGAGA8420.15172428705802643No Hit
GTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCA8390.15118370171221399No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8190.1475797994067977No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8010.144336287331923No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7850.14145316548758996No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7820.14091258014177752No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATCCAGTGGGGATGTTGTGATGACTCAGTCTCCTCTCTCC7710.13893043387379855No Hit
GGTCTAGTCAAGGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCCAGGCCAATCTC7630.13748887295163204No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATGGGGTTT7410.13352458041567408No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7350.1324434097240492No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6930.12487521488267496No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATCCAGTGGGGATGTTGTGATGACTCAGTCTCCTCT6900.12433462953686251No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6850.12343365396050843No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6770.12199209303834192No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6750.12163170280780028No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6690.1205505321161754No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6670.12019014188563376No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6630.1194693614245505No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6590.11874858096346726No Hit
CTGTATACGAGGCCTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC6590.11874858096346726No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6540.11784760538711317No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6500.11712682492602991No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6470.11658623958021747No Hit
AGTCAAGGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCCAGGCCAATCTCCAAGG6280.11316253239007197No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6220.11208136169844707No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6170.11118038612209301No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT6060.10919823985411405No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6040.1088378496235724No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6020.10847745939303076No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6000.10811706916248914No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5950.10721609358613506No Hit
GCTGATGTTCAGTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGT5940.10703589847086425No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5930.10685570335559344No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA5920.10667550824032261No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5900.106315118009781No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5840.1052339473181561No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5810.10469336197234365No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5800.10451316685707285No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5780.1041527766265312No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC5780.1041527766265312No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCTGCTCCCAGATGGGCC5770.10397258151126039No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5750.10361219128071876No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5730.10325180105017714No Hit
GGTGCACACTGGCCCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT5600.10090926455165654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAG300.003163267264.042170
TATACCG200.0079177650.7973825
TAGTTCG452.1160522E-645.145097
ACGGAAA358.9020905E-442.89269667
AGTTCGC504.367488E-640.630588
CGTATGC2600.039.90559841
ATCTCCG2650.039.60842510
GTATGCC2750.039.0620542
TAGTGTG350.001476032938.7027635
ATGCCGT2750.037.9103544
TCGTATG2650.037.77456740
GTATCAA12000.037.2648431
TATACAC3100.037.142173
AGACCGA2750.037.0135925
CTGATCT2500.036.96047234
GTGGTAT1650.036.9568671
ACCGAGG2850.036.92870727
CCCACGA2850.036.83552619
ATCTCGT2550.036.38818737
GATCTCG2550.036.32507336
Produced by FastQC (version 0.11.5)