FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554954
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26760.4822021284647016No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23600.425260472039124No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17610.3173235979919056No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAGGCCTCGTA17510.3155216468391975No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17370.31299891522540607No Hit
GTGCACACTGGCCCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA16300.29371803789142886No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14710.26506701456336923No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14300.2576790148372658No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13490.24308321050032977No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13490.24308321050032977No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12950.23335267427570575No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12900.23245169869935164No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCCAG12350.22254096735945683No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12320.2220003820136444No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12090.21785589436241565No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC11600.2090263337141457No Hit
GTCTGGGACCCCAGGGTCCCGGTAAGAAACCCCATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGGTGAAA11380.20506204117818772No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11300.2036204802560212No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11280.2032600900254796No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11210.20199872421858386No Hit
GATTTCACACTGAACATCAGCAGGGTGGAGGCTGAGGATGTTGGGATTTATTTCTGCATGCAAGGTGCACACTGG11060.19929579748952167No Hit
GTGTGCACCTTGCATGCAGAAATAAATCCCAACATCCTCAGCCTCCACCC11020.19857501702843838No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCC10770.194070139146668No Hit
GTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCA10670.19226818799395987No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10380.1870425296511062No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10170.1832584322304191No Hit
AGTGTGCACCTTGCATGCAGAAATAAATCCCAACATCCTCAGCCTCCACC9860.1776723836570238No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9700.17478926181269078No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA9600.17298731065998263No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9580.172626920429441No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9560.17226653019889937No Hit
GAACATCAGCAGGGTGGAGGCTGAGGATGTTGGGATTTATTTCTGCATGCAAGGTGCACACTGGCCCCCCACTTT8950.16127462816737964No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8920.1607340428215672No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8860.1596528721299423No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8750.15767072586196335No Hit
CCTCTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG8680.15640936005506764No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG8240.14848077498315176No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATCCAGTGGGGATGTTGTGATGA8220.14812038475261013No Hit
GTCCAAGGGTGACGGGCAGGGAGAGAGGAGACTGAGTCATCACAACATCC8200.1477599945220685No Hit
GTATACGAGGCCTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGAGGAGA7890.14217394594867322No Hit
GGTCTAGTCAAGGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCCAGGCCAATCTC7690.13857004364325692No Hit
GCTAATGCTCTGGGTCCCAGCATCCAGTGGGGATGTTGTGATGACTCAGT7470.13460575110729897No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATCCAGTGGGGATGTTGTGATGACTCAGTCTCCTCTCTCC7260.13082165368661186No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7240.13046126345607023No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7110.12811872695754964No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7060.12721775138119557No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7040.12685736115065394No Hit
GCTGATGTTCAGTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGT7010.12631677580484146No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6980.12577619045902902No Hit
GGGTGGAGGCTGAGGATGTTGGGATTTATTTCTGCATGCAAGGTGCACACTGGCCCCCCACTTTCGGCGGAGGGA6910.12451482465213333No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6860.12361384907577926No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATGGGGTTT6740.12145150769252946No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6710.12091092234671702No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6650.11982975165509213No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATCCAGTGGGGATGTTGTGATGACTCAGTCTCCTCT6600.11892877607873806No Hit
CTGTATACGAGGCCTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC6500.11712682492602991No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6490.1169466298107591No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6480.11676643469548827No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6380.11496448354278012No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6300.1135229226206136No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6260.11280214215953034No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA6160.1110001910068222No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6120.11027941054573892No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6090.10973882519992649No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC6000.10811706916248914No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5960.10739628870140588No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5880.10595472777923935No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5860.10559433754869774No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5830.10505375220288529No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5820.10487355708761446No Hit
AGTCAAGGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCACCAGAGGCCAGGCCAATCTCCAAGG5800.10451316685707285No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5750.10361219128071876No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT5730.10325180105017714No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5700.10271121570436469No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5670.10217063035855224No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5670.10217063035855224No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5630.10144984989746898No Hit
GGTGCACACTGGCCCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT5630.10144984989746898No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATGGGGTTTCTTACCGGGAC5590.10072906943638571No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5580.1005488743211149No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT250.00132739979.7155970
TGGTATA200.00794591850.7517782
GTGGTAT2450.038.7029041
TGGTATC2450.038.6680222
TGTTAGA6500.036.7918170
GTATCAA14300.036.4700471
GGTATCA6050.035.2644231
GAATACG502.0901214E-433.8223279
ATCGGTG450.00402971531.5361154
ATACGTA652.6561354E-531.22061211
TATCAAC16800.031.2163732
AATGGTA2151.2350938E-930.89751470
ATCAACG17100.030.6631893
TCAACGC17100.030.2648094
CAACGCA17350.029.8260275
AACGCAG17350.029.8260276
AGAGTAC18150.029.25677911
GCAGAGT18050.029.2314859
CAGAGTA17950.029.2059110
CGCAGAG17900.029.0985378
Produced by FastQC (version 0.11.5)