FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495457
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136730.9143024506889866No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTATGCATTTGCAGATACA52980.35427297474952474No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44530.29776850822190143No Hit
GTATATTACTGTGCGGGATCGGGGAGAGGCAGTGCTGCGGAGAGTTATTT40210.2688810176420987No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40140.26841293330400007No Hit
GGACTGAACAGAGAGAACTCACCATGGAGGTTGGGCTGCGCTGGCTTTTT36940.24701479213377586No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36530.24427315529634086No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34210.2287595029479283No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30220.20207869567630496No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29310.19599359928102245No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27290.18248602266731842No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27000.18054681612376686No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26890.1798112550210404No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26550.1775377025217041No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTGTTTCTACATATTTAAATTGGTATCAGCAGAAA26140.1747960656842691No Hit
CTCCTGTGCAGCCTCTGGATTCAGGTTGAACAATTATGCCATGACCTGGCTGCGCCAGGCTCCAGGGAAGGGG25520.17065017583253814No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24720.16530064053998209No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24400.16316082642295968No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23990.1604191895855247No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23790.15908180576238568No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23170.15493591591065473No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23130.15466843914602693No Hit
CTCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGGGTCTCAGCACCCCAGAGCTCA22540.15072315686776686No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22230.14865021194190137No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21620.1445711912813274No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21490.143701891796287No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20810.13915478679761437No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20810.13915478679761437No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20430.13661375753365027No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19990.13367151312274442No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19940.13333716716695967No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC19860.13280221363770406No Hit
GTGGGGTTCCATCCAGGTTCAGTGGCGGTGGATCAGGGACAGATTTCAGT19850.1327353444465471No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19830.1326016060642332No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18920.1265165096689507No Hit
CCCCAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACT18880.12624903290432288No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18750.12537973341928255No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18030.12056515165598208No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17890.11962898297978478No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17770.11882655268590137No Hit
ACTTACTACTGTCAGCAGGGTCTCAGCACCCCAGAGCTCACTTTCGGCGG17720.11849220673011661No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17680.11822472996548881No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17620.1178235148185471No Hit
GTTTACTACTTGTCCTATGGTCTGGCCGTCTGGGGCCAAGGGACCACGGT17340.11595117746615248No Hit
GATTTCAGTCTCTCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGGGTCTCAGCACC17260.11541622393689689No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17190.11494813959879824No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCTCTGGGGTGCTGAGACCCTG16800.11234024114367715No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGCAGCCAGGTCATGGCATAATTGT16590.11093598812938119No Hit
GAGTTATTTCCGTCTCGGGGAAGTTTACTACTTGTCCTATGGTCTGGCCGTCTGGGGCCAAGGGACCACGGTCAC16460.11006668864434081No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTATGCATTTGCAGATA16170.10812748210078926No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16110.10772626695384754No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16050.10732505180690585No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15840.10592079879260989No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15830.10585392960145293No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15770.10545271445451122No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGGTTGAACAATTATGCCATGACCTGGCTGCG15760.10538584526335427No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTCC15700.10498463011641257No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15620.10444967658715697No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15240.10190864732319285No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15110.10103934783815248No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15110.10103934783815248No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGAGAGACTGAAATCTGTCCCTGATCCACCGCCA15050.10063813269121079No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15020.10043752511773993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGC307.2018866E-444.7849968
TCTGAAG17200.044.51518270
GTGGTAT3450.039.9464531
GTATCAA27350.036.2558561
TATCAAC31500.031.1435242
ATCAACG31550.030.9752483
TCAACGC32050.030.7026084
AACGCAG32400.030.3729766
CAACGCA32550.030.2319985
GACAGGT3200.029.4117931
ACGCAGA34250.028.8304567
CGCAGAG34600.028.3465718
AGAGTAC35250.027.9182211
GGCGATC656.646085E-427.76727757
GCAGAGT35650.027.510769
CTCTGAA21750.027.50336869
GCTTGGG1354.1472958E-1027.388761
TGTCTCT18950.026.95966766
GACAGCC2500.026.8907831
TACTTGT20200.026.83470370
Produced by FastQC (version 0.11.5)