FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249118
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC61880.4953895468642674No Hit
GCCTTGGGCTGACCTACGACGGTCACCTTGGTCCCTCCGCCGAAGACCCAAGTGTCACTACCTGCATAACAACAG57570.4608852005975416No Hit
GTCCTGGGCCCAGTCCGTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCATG50730.40612656290278415No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46670.3736236288325042No Hit
CTCCTGGACAGTCGATCACCATCTCATGCACTGGAACCAGCAGTGATCTT40840.32695069641138785No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39410.31550261864771784No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38220.30597589659263574No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCACCTTGGTCCCTC37100.2970095699525585No Hit
GACCTACGACGGTCACCTTGGTCCCTCCGCCGAAGACCCAAGTGTCACTACCTGCATAACAACAGCAGTAATAAT35100.280998272380992No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34960.27987748155098235No Hit
GTCGATCACCATCTCATGCACTGGAACCAGCAGTGATCTTGGCAGTTACA34560.27667522203666906No Hit
GATTATTACTGCTGTTGTTATGCAGGTAGTGACACTTGGGTCTTCGGCGGAGGGACCAAGGTGACCGTCGTAGGT34540.27651510906095345No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT33950.2717917762773413No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG33170.26554737022443037No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32580.2608240374408182No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31320.25073691997073133No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30640.24529307879639872No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29490.23608658269274801No Hit
CCCTGACAGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC29450.23576635674131666No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28560.22864132932196957No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28550.22856127283411176No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28500.22816099039482257No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28200.2257592957590876No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28020.2243182789776466No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27010.21623257370400553No Hit
GGACACAGAGTCCTGGGCCCAGTCCGTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25730.205985343258203No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25140.20126201047459086No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTCCGTCCTGACTCAGC25100.20094178452315956No Hit
GTTACACCCTTGTCTCCTGGTACCAACAACAACCAGGCAAAGCCCCCAAA24990.20006116315672337No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACTGTCAGGGAGG24140.1932563616888076No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23910.19141506246807746No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23400.18733218158732803No Hit
CTCATGCACTGGAACCAGCAGTGATCTTGGCAGTTACACCCTTGTCTCCTGGTACCAACAACAACCAGGCAAAGC23250.18613133426946055No Hit
CCTTGGTCCCTCCGCCGAAGACCCAAGTGTCACTACCTGCATAACAACAGCAGTAATAATCAGCCTCGTCCTCAG22870.1830891877308629No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22730.18196839690085323No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22460.17980687172869178No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21810.17460320001793267No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21520.1722815618700555No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCACCTTGGTCCCTCCGCCGAAGAC21130.16915935884360003No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21030.16835879396502174No Hit
CTGTCAGGGAGGCCCTGATTCCAGACTTGGAGCCAGAGAAGCGACTTGATACCCCTGAGGGTCGCTTACTGCCCT20700.16571692986571324No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20090.16083348410638548No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19420.15546969941991068No Hit
GTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19410.15538964293205285No Hit
CTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTCCGTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA19270.15426885210204322No Hit
CCCTTGTCTCCTGGTACCAACAACAACCAGGCAAAGCCCCCAAACTCTTGATTTTTGAGGGCAGTAAGCGACCCT18220.14586292087697078No Hit
CCCCTGAGGGTCGCTTACTGCCCTCAAAAATCAAGAGTTTGGGGGCTTTGCCTGGTTGTTGTTGGTACCAGGAGA17930.14354128272909367No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17710.14178003999622135No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17700.1416999835083635No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCACCTTGGTCCCTCCGCCGAAGACCCAAGTGTCA17600.14089941862978517No Hit
GGACCAAGGTGACCGTCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG17440.13961851482405985No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTCCGTCCTGACT17430.13953845833620204No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17340.13881794994548155No Hit
GTCAGGGAGGCCCTGATTCCAGACTTGGAGCCAGAGAAGCGACTTGATAC17280.13833761101833453No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17150.13729687667618273No Hit
CCTCAGGGGTATCAAGTCGCTTCTCTGGCTCCAAGTCTGGAATCAGGGCCTCCCTGACAGTCTCTGGGCTCCAGG16800.13449489960115857No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16790.13441484311330074No Hit
GCCTGGAGCCCAGAGACTGTCAGGGAGGCCCTGATTCCAGACTTGGAGCC16510.13217326145328143No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16350.1308923576475561No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16040.1284106065239633No Hit
GCAGTGATCTTGGCAGTTACACCCTTGTCTCCTGGTACCAACAACAACCA15980.1279302675968163No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC15610.12496817754607652No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTC15590.12480806457036085No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGC15590.12480806457036085No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTCCGTCCTGACTCAGCCTGCCT15540.12440778213107168No Hit
GTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGTTGTTA15160.12136563559247404No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15150.12128557910461622No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15120.12104540964104273No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15030.12032490125032222No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCATGCA14810.1185636585174499No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14720.11784315012672941No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14610.11696252876029327No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGTTGTTATGCAGGTA14580.11672235929671977No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC14540.11640213334528843No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14440.1156015684667101No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14360.11496111656384746No Hit
GTCTGGAATCAGGGCCTCCCTGACAGTCTCTGGGCTCCAGGCTGAGGACG14280.11432066466098478No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTCCGT14200.11368021275812214No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14040.1123993089523968No Hit
GTAAGCGACCCTCAGGGGTATCAAGTCGCTTCTCTGGCTCCAAGTCTGGA13960.11175885704953416No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13950.11167880056167633No Hit
GGGTGTAACTGCCAAGATCACTGCTGGTTCCAGTGCATGAGATGGTGATC13630.10911699295022567No Hit
CTCCCTGACAGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGTTGTTATGCAGGTAGTGACA13630.10911699295022567No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCACCTTGGTCCCTCCGCCGAAGACC13620.10903693646236784No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC13560.10855659753522084No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13330.10671529831449071No Hit
GTTATGCAGGTAGTGACACTTGGGTCTTCGGCGGAGGGACCAAGGTGACCGTCGTAGGTCAGCCCAAGGCTGCCC13130.10511416855733405No Hit
CTTGTCTCCTGGTACCAACAACAACCAGGCAAAGCCCCCAAACTCTTGAT13090.10479394260590272No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13090.10479394260590272No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12830.10271247392159909No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC12820.10263241743374124No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG12670.10143157011587377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC150.002478766368.118733
TCGTATG1550.053.38459840
ATCTCGT1600.049.3674337
AGACTGC1950.048.937125
CGTATGC1650.048.13178341
ACGAGAC1950.047.16478322
AATCTCG1700.046.42224536
GTGGTAT3150.045.417941
GCTAATC1750.045.00152633
CGAGACT2050.044.8658623
CACGAGA2000.044.27894621
TCTCGTA1650.043.76878438
TGGTATC3550.043.1738432
AGGGGGG2500.042.9512370
TATACAC2100.042.168743
CTAATCT1900.041.4738134
ATGCCGT1950.040.9128544
CCGTCTT1800.040.6637747
ATCTCCG2200.040.25197610
CGAGCCC2250.039.35748715
Produced by FastQC (version 0.11.5)