FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249118
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC61660.4936283041313951No Hit
GCCTTGGGCTGACCTACGACGGTCACCTTGGTCCCTCCGCCGAAGACCCAAGTGTCACTACCTGCATAACAACAG53900.4315044695537171No Hit
GTCCTGGGCCCAGTCCGTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCATG53550.42870249247869296No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49420.3956391629934082No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38410.3074969698619346No Hit
CTCCTGGACAGTCGATCACCATCTCATGCACTGGAACCAGCAGTGATCTT37960.3038944279083321No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36500.29220618068108856No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36140.2893241471182066No Hit
GATTATTACTGCTGTTGTTATGCAGGTAGTGACACTTGGGTCTTCGGCGGAGGGACCAAGGTGACCGTCGTAGGT35100.280998272380992No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33850.270991211398763No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT33410.26746872593301835No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCACCTTGGTCCCTC33290.26650804807872436No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32380.2592229076836616No Hit
GTCGATCACCATCTCATGCACTGGAACCAGCAGTGATCTTGGCAGTTACA32250.2581821733415097No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31980.25602064816934833No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31880.25522008329076995No Hit
GACCTACGACGGTCACCTTGGTCCCTCCGCCGAAGACCCAAGTGTCACTACCTGCATAACAACAGCAGTAATAAT31440.2516975978250253No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30560.24465262689353606No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29160.23344471859343954No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTCCGTCCTGACTCAGC28520.22832110337053824No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27740.22207669731762733No Hit
CCCTGACAGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC27660.22143624541476467No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26970.21591234775257423No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26910.21543200882542723No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25680.2055850608189138No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCACCTTGGTCCCTCCGCCGAAGAC25520.2043041570131885No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25440.20366370511032583No Hit
GGACACAGAGTCCTGGGCCCAGTCCGTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24800.19854008988742458No Hit
GTTACACCCTTGTCTCCTGGTACCAACAACAACCAGGCAAAGCCCCCAAA24760.19821986393599322No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23460.18781252051447503No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23100.18493048695159303No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22640.18124788851013274No Hit
CTCATGCACTGGAACCAGCAGTGATCTTGGCAGTTACACCCTTGTCTCCTGGTACCAACAACAACCAGGCAAAGC21960.17580404733580016No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21560.17260178782148686No Hit
CCTTGGTCCCTCCGCCGAAGACCCAAGTGTCACTACCTGCATAACAACAGCAGTAATAATCAGCCTCGTCCTCAG21550.172521731333629No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACTGTCAGGGAGG21540.17244167484577116No Hit
GTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC21520.1722815618700555No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21050.16851890694073737No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20920.16747817259858555No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20760.16619726879286023No Hit
CTGTCAGGGAGGCCCTGATTCCAGACTTGGAGCCAGAGAAGCGACTTGATACCCCTGAGGGTCGCTTACTGCCCT20110.16099359708210112No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19810.15859190244636617No Hit
CTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTCCGTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA19610.1569907726892095No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19360.15498936049276368No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19350.15490930400490585No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18950.15170704449059255No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTC18350.1469036552191226No Hit
CCCTTGTCTCCTGGTACCAACAACAACCAGGCAAAGCCCCCAAACTCTTGATTTTTGAGGGCAGTAAGCGACCCT18180.14554269492553948No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCACCTTGGTCCCTCCGCCGAAGACCCAAGTGTCA18060.1445820170712455No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18020.14426179111981413No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTCCGTCCTGACT17970.14386150868052497No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTCCGTCCTGACTCAGCCTGCCT17720.14186009648407916No Hit
CCCCTGAGGGTCGCTTACTGCCCTCAAAAATCAAGAGTTTGGGGGCTTTGCCTGGTTGTTGTTGGTACCAGGAGA17370.13905811940905505No Hit
GGACCAAGGTGACCGTCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG17110.13697665072475138No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16820.13465501257687423No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16810.1345749560890164No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16480.13193309198970793No Hit
GGGTGTAACTGCCAAGATCACTGCTGGTTCCAGTGCATGAGATGGTGATC16350.1308923576475561No Hit
CCTCAGGGGTATCAAGTCGCTTCTCTGGCTCCAAGTCTGGAATCAGGGCCTCCCTGACAGTCTCTGGGCTCCAGG16110.12897100193896813No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCACCTTGGTCCCTCCGCCGAAGACC16050.12849066301182113No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCATGCA16000.12809038057253197No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGC15900.12728981569395367No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT15880.12712970271823798No Hit
GCAGTGATCTTGGCAGTTACACCCTTGTCTCCTGGTACCAACAACAACCA15830.12672942027894882No Hit
GTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGTTGTTA15560.12456789510678734No Hit
GTCAGGGAGGCCCTGATTCCAGACTTGGAGCCAGAGAAGCGACTTGATAC15340.12280665237391504No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15110.12096535315318489No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14980.11992461881103307No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14960.1197645058353174No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14960.1197645058353174No Hit
GGGGTATCAAGTCGCTTCTCTGGCTCCAAGTCTGGAATCAGGGCCTCCCT14660.11736281119958242No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14490.11600185090599928No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14220.11384032573383779No Hit
GTCTGGAATCAGGGCCTCCCTGACAGTCTCTGGGCTCCAGGCTGAGGACG14190.11360015627026429No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGAGTCCTGGGCCCAGTCCGT14150.11327993031883297No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14130.1131198173431173No Hit
GCCTGGAGCCCAGAGACTGTCAGGGAGGCCCTGATTCCAGACTTGGAGCC14080.11271953490382815No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGTTGTTATGCAGGTA13720.10983750134094616No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC13540.10839648455950518No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC13420.1074358067052112No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13380.10711558075377986No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13290.10639507236305937No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13280.10631501587520154No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13270.10623495938734372No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13270.10623495938734372No Hit
CTTGTCTCCTGGTACCAACAACAACCAGGCAAAGCCCCCAAACTCTTGAT13210.10575462046019672No Hit
CTCCCTGACAGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGTTGTTATGCAGGTAGTGACA13150.10527428153304973No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTC13070.10463382963018705No Hit
CACTTGGGTCTTCGGCGGAGGGACCAAGGTGACCGTCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT12920.10343298231231958No Hit
GTAAGCGACCCTCAGGGGTATCAAGTCGCTTCTCTGGCTCCAAGTCTGGA12820.10263241743374124No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12820.10263241743374124No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12730.10191190904302076No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC12620.10103128767658459No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC12540.10039083577372193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)