FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604091
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAGGGCCAGTGGCATTCCAGACAGGTTCAGTGGCGGTGGGTCTGGGA25600.4237772123736325No Hit
GTACTACTGTCAGAACTATGGTGGGGTACAGCGGCCGTTCGGCCAAGGGA24330.4027538897285343No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24080.39861544038894803No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22720.3761022759815988No Hit
GTTAGGGCCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA19450.3219713586198106No Hit
GTGTACTACTGTCAGAACTATGGTGGGGTACAGCGGCCGTTCGGCCAAGGGACCAAGGTGGAGATCAAGCGAA18520.30657632707654975No Hit
ATAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGAAACA16840.2787659475145301No Hit
GTCTGGGACAGACTTCAGTCTCACCATCAGCAGACTGGAGCCTGAAGATT16690.2762828779107783No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15470.25608724513359743No Hit
GTAGTACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACCGCCA15440.2555906312128471No Hit
CAGTAGTACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACCGCCA15010.24847249834875873No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14820.24532727685067315No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13120.21718582134148662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12820.21221968213398315No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12240.20261847966614302No Hit
GTATATTATTGTGCGAAAGGGGGCGGGAGCAACAGCTGGTTGGAGGACTA12090.20013541006239127No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12020.19897664424730713No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGAATGCCACTGGCCCTGGT11960.1979834164058064No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTATTGACGA11960.1979834164058064No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11710.19384496706622018No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11310.18722344812288216No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11290.18689237217571525No Hit
CTACTACTTAGCCTGGTTCCAACAAAAACCTGGCCAGCCTCCCAGACTCCTCATCTATGACGCACACACCAGGGC11260.1863957582549649No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10810.17894654944370963No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGA10720.17745670768145858No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10460.17315272036828888No Hit
GCCCTGGTGTGTGCGTCATAGATGAGGAGTCTGGGAGGCTGGCCAGGTTT10430.17265610644753854No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9980.1652068976362833No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9970.16504135966269984No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9820.1625582900589481No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9740.1612339862702805No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGTGACTTCACCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG9520.15759215085144457No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTTGGCCGAACGGC9110.1508050939345231No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCGATTTTAGAAGGTGTCCAATGTGAGGTGCACCTGGTGGAGT9000.14898417622510515No Hit
GCTCAGTTAGGGCCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA8940.14799094838360446No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8820.14600449270060306No Hit
CTCCCAGACTCCTCATCTATGACGCACACACCAGGGCCAGTGGCATTCCA8750.1448457268855189No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8620.14269373322893406No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8350.13822420794218088No Hit
GAGTATTGACGACTACTACTTAGCCTGGTTCCAACAAAAACCTGGCCAGC8290.13723098010068022No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8100.13408575860259464No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC8040.13309253076109395No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7990.1322648408931767No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC7900.13077499913092563No Hit
CAATAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGAAA7870.1302783852101753No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACCGGAGAAAATGTGTTGACGCAGTCTC7780.12878854344792423No Hit
GTCTGGGAGGCTGGCCAGGTTTTTGTTGGAACCAGGCTAAGTAGTAGTCG7710.12762977763284009No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7650.1266365497913394No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7500.12415348018758764No Hit
GAACTATGGTGGGGTACAGCGGCCGTTCGGCCAAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT7460.12349132829325384No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7450.12332579031967038No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7420.12282917639892003No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7380.12216702450458623No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG7290.12067718274233517No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7270.12034610679516827No Hit
GACTTCAGTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTACTACTGTCAGAACTATGGTGGGGTA7180.11885626503291723No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7170.11869072705933377No Hit
CTCCTGCAAGGCCAGTCAGAGTATTGACGACTACTACTTAGCCTGGTTCC7120.11786303719141653No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC7100.11753196124424962No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6740.11157259419524541No Hit
GGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC6680.11057936635374471No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6580.10892398661791021No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6540.10826183472357641No Hit
GTGCTGGGTTTTCCTTGTTGCGATTTTAGAAGGTGTCCAATGTGAGGTGC6530.10809629674999295No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6530.10809629674999295No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6500.10759968282924261No Hit
CCATAGTTCTGACAGTAGTACACTGCAAAATCTTCAGGCTCCAGTCTGCT6410.10610984106699158No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6410.10610984106699158No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6380.10561322714624122No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT6350.10511661322549087No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6290.10412338538399017No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6240.10329569551607291No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAAAATGTGTTGACGCAGTC6230.10313015754248947No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6170.10213692970098877No Hit
CTACGCAGACTCTGTGAAGGGCCGCTTCACCATCTCCAGAGACAATGCCAAGAACTTACTGTTTCTGCAAATGAA6140.10164031578023841No Hit
GTACAGCGGCCGTTCGGCCAAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTC6120.10130923983307151No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATGGTGAAGTCAC6080.10064708793873771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)