FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004688722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604091
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTACTGTCAGAACTATGGTGGGGTACAGCGGCCGTTCGGCCAAGGGA24770.4100375605662061No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23440.3880210100796072No Hit
CACCAGGGCCAGTGGCATTCCAGACAGGTTCAGTGGCGGTGGGTCTGGGA22840.3780887316646002No Hit
GTTAGGGCCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA18280.30260341571054694No Hit
GTGTACTACTGTCAGAACTATGGTGGGGTACAGCGGCCGTTCGGCCAAGGGACCAAGGTGGAGATCAAGCGAA17920.2966440486615427No Hit
ATAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGAAACA17870.29581635879362544No Hit
GTCTGGGACAGACTTCAGTCTCACCATCAGCAGACTGGAGCCTGAAGATT16940.28042132725036456No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16400.2714822766768583No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16360.2708201247825245No Hit
GTAGTACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACCGCCA14970.2478103464544249No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13880.22976670733382884No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13350.22099319473390597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13240.21917227702448802No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12930.21404059984340107No Hit
CAGTAGTACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACCGCCA12650.2094055365830645No Hit
GTATATTATTGTGCGAAAGGGGGCGGGAGCAACAGCTGGTTGGAGGACTA12450.20609477711139548No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11630.19252066327755257No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11520.1906997455681346No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGA11110.18391268865121316No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTATTGACGA11100.1837471506776297No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11050.18291946080971244No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10760.17811885957579238No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGAATGCCACTGGCCCTGGT10570.17497363807770683No Hit
CTACTACTTAGCCTGGTTCCAACAAAAACCTGGCCAGCCTCCCAGACTCCTCATCTATGACGCACACACCAGGGC10400.17215949252678817No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10020.1658690495306171No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9930.16437920776836604No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9710.16073737234953012No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGTGACTTCACCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG9450.15643338503636042No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9420.15593677111561008No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9340.15461246732694248No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9290.15378477745902522No Hit
GCCCTGGTGTGTGCGTCATAGATGAGGAGTCTGGGAGGCTGGCCAGGTTT9240.15295708759110796No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9140.15130170785527347No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9000.14898417622510515No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTTGGCCGAACGGC8950.1481564863571879No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCGATTTTAGAAGGTGTCCAATGTGAGGTGCACCTGGTGGAGT8950.1481564863571879No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8800.14567341675343615No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT8460.14004512565159885No Hit
CTCCCAGACTCCTCATCTATGACGCACACACCAGGGCCAGTGGCATTCCA8430.13954851173084848No Hit
GCTCAGTTAGGGCCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA8420.13938297375726505No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8220.13607221428559604No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACCGGAGAAAATGTGTTGACGCAGTCTC8030.1329269927875105No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7970.1319337649460098No Hit
GAGTATTGACGACTACTACTTAGCCTGGTTCCAACAAAAACCTGGCCAGC7890.1306094611573422No Hit
GTGCTGGGTTTTCCTTGTTGCGATTTTAGAAGGTGTCCAATGTGAGGTGC7820.12945069534225803No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7680.12713316371208974No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7680.12713316371208974No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAAAATGTGTTGACGCAGTC7640.12647101181775594No Hit
CAATAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGAAA7200.11918734098008413No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7100.11753196124424962No Hit
GAACTATGGTGGGGTACAGCGGCCGTTCGGCCAAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT7060.11686980934991582No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC7010.11604211948199857No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6980.11554550556124822No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC6830.11306243595749646No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6830.11306243595749646No Hit
GTCTGGGAGGCTGGCCAGGTTTTTGTTGGAACCAGGCTAAGTAGTAGTCG6620.10958613851224401No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6500.10759968282924261No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG6410.10610984106699158No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6250.10346123348965637No Hit
AAGTAGTAGTCGTCAATACTCTGACTGGCCTTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTG6170.10213692970098877No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6170.10213692970098877No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA6080.10064708793873771No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6060.10031601199157081No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6060.10031601199157081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)