FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948667
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATCCA50990.5374910268829842No Hit
CAAATATACTATGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTCCAAGAACACACTGGATCTG33410.35217837238989025No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31610.3332043804622697No Hit
GGCCAGGAGCGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC28770.3032676376431351No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28310.2984187285949653No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28160.296837562600997No Hit
CTCCTGGCCAGTCCCTACGCTGGTGACAGTAATAAACTGCAAAATCTTCA26300.2772311042757891No Hit
GTATATTTGAGCGCCAGTACCACTAATAAGTGAGACCCACTCTAGCCCCTTTCCTGGAGCCTGACGGACCCAGCT25870.27269842842641306No Hit
GCGTAGGGACTGGCCAGGAGCGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCAT25220.26584670911921676No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22530.2374911322940505No Hit
GTATGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATT22500.23717489909525682No Hit
GCTTAATACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAACCTCTGGA21200.2234714604808642No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC21020.22157406128810214No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20930.22062536169172114No Hit
GTGTTAGTACCTACTTAGCCTGGTACCAAAAAAAACCTGGCCAGTCTCCC20840.2196766620953401No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20660.217779262902578No Hit
CAGTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATC20400.21503857517969951No Hit
GGCTTAATACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAACCTCTGG20340.21440610878211216No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18650.19659163858340178No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18410.19406177299305236No Hit
GTCCCTACGCTGGTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGT17960.18931827501114723No Hit
GTATATTACTGTGTGAAAGATGGGCCCTACCATGGAGACTTCCGGATGGG17650.1860505319569459No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17410.18352066636659647No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17290.1822557335714218No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17000.17919881264974957No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16880.1779338798545749No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16810.17719600239072297No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16530.17424449253531535No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16030.1689739392220874No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15870.16728736216185447No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAC15720.1657061961678861No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15510.16349256377633037No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15440.16275468631247847No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15350.16180598671609744No Hit
GTTTATTACTGTCACCAGCGTAGGGACTGGCCAGGAGCGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAA15330.16159516458356832No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15140.1595923543245417No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15130.15948694325827714No Hit
GTCCCATACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTTTT15080.15895988792695434No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15040.15853824366189612No Hit
GTATATTACTGTGTGAAAGATGGGCCCTACCATGGAGACTTCCGGATGGGAGGTTTTGATGTCTGGGGCCCAGGG14990.15801118833057332No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14930.15737872193298597No Hit
ATATTACTGTGTGAAAGATGGGCCCTACCATGGAGACTTCCGGATGGGAG14880.15685166660166316No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14830.1563246112703404No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14780.1557975559390176No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14680.15474344527637202No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14440.1522135796860226No Hit
GTATTAAGCCTCCCCCAGACTCCACCACCTGCACCTCACATTGGACACCT14390.1516865243546998No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTACCTACTTAGCCTGGTA14300.1507378247583188No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14100.1486296034330276No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13780.14525644931256174No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13670.14409692758365159No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13450.1417778841258313No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13370.14093459559571483No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13100.13808849680657173No Hit
GACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTAGGGACTGG12980.13682356401139706No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12970.13671815294513248No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTGGG12640.13323958775840206No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAATGTGAGGTGCAGGTGGTGGAGT12630.13313417669213748No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12530.1320800660294919No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12520.13197465496322733No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTACCTACTTAGCCTGGTACCAAAAAAAACCTGGCCAGTCTCCCAGGCT12390.13060431110178808No Hit
ACCATGGAGACTTCCGGATGGGAGGTTTTGATGTCTGGGGCCCAGGGACAATGGTCACCGTCTCTTCAGCATCCC12320.12986643363793618No Hit
CTCTAGCCCCTTTCCTGGAGCCTGACGGACCCAGCTCATGAAATAAGTTC12300.12965561150540705No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12280.12944478937287793No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCATACCCACTGCCA12280.12944478937287793No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12150.12807444551143868No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTCGGTCCCTTGGCCGAACGCTCCTGGCCAGTC12020.12670410164999943No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11910.12554457992108928No Hit
GGGTATGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAG11760.1239634139271209No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT11700.12333094752953354No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11490.12111731513797781No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11330.11943073807774487No Hit
CCATTGTCCCTGGGCCCCAGACATCAAAACCTCCCATCCGGAAGTCTCCA11280.11890368274642209No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG11270.11879827168015752No Hit
GGGCTAGAGTGGGTCTCACTTATTAGTGGTACTGGCGCTCAAATATACTA11260.11869286061389298No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11190.11795498315004106No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11140.11742792781871826No Hit
CCCTGAGACTCTCCTGTGCAACCTCTGGATTCGCCTTTGGAACTTATTTC11130.1173225167524537No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT10860.11447641796331062No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTGGG10850.11437100689704607No Hit
CTCCCAGGCTCCTCATCTATGATACATCCAAAAGGGCCACTGGCATCCCA10840.11426559583078151No Hit
GAGTGAAGTCTGTCCCATACCCACTGCCACTGAACCTGGCTGGGATGCCA10810.11394936263198782No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10700.1127898409030777No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10650.11226278557175488No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG10650.11226278557175488No Hit
CTCCTGTGCAACCTCTGGATTCGCCTTTGGAACTTATTTCATGAGCTGGG10640.11215737450549032No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10550.11120867490910932No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA10450.11015456424646372No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT10390.10952209784887638No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10350.10910045358381813No Hit
GTCAGGTACTTCTCGCGGGGCAGCTCCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG10330.108889631451289No Hit
GTCTGGGGGAGGCTTAATACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAACCTCTGGATTCGCCTTTGGAA10320.10878422038502446No Hit
GCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC10320.10878422038502446No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA10280.10836257611996623No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10010.10551647733082314No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT9810.10340825600553198No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9800.10330284493926742No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9770.10298661174047373No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG9670.10193250107782814No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9660.1018270900115636No Hit
GTCAGAGTGTTAGTACCTACTTAGCCTGGTACCAAAAAAAACCTGGCCAGTCTCCCAGGCTCCTCATCTATGATA9650.10172167894529904No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9570.10087839041518258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATCG353.4213008E-758.79994234
ATACGGT252.7251506E-454.5356986
CCTCGAC200.00660419353.18498258
ATCGCGT200.007449537551.58951637
TACGGTC200.007722649351.121837
CGCGTAC351.933084E-550.65236358
CCGCTAT554.92817E-448.36191670
CCGCGTA305.5537483E-447.20823757
TCTGGAG14950.045.81442670
TACACAT1650.045.446425
ATCTCCG1650.045.44162810
GGTATCA6550.044.2438661
GTGGTAT1700.044.1213841
CGAGACT1700.044.11673423
ACACATC1800.043.552826
CCCACGA1650.043.376119
GCATCGC405.1468207E-542.91593635
GAGACTA1750.042.86303724
ACTACGC1600.042.65548727
CTTATAC1700.042.1158681
Produced by FastQC (version 0.11.5)