FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948667
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATCCA41770.4403020237870612No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31050.32730136075145444No Hit
CAAATATACTATGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTCCAAGAACACACTGGATCTG30180.31813059798643784No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT26290.2771256932095245No Hit
CTCCTGGCCAGTCCCTACGCTGGTGACAGTAATAAACTGCAAAATCTTCA26040.27449041655291057No Hit
GTATATTTGAGCGCCAGTACCACTAATAAGTGAGACCCACTCTAGCCCCTTTCCTGGAGCCTGACGGACCCAGCT25560.2694306853722117No Hit
GGCCAGGAGCGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC24800.2614194443361053No Hit
GCGTAGGGACTGGCCAGGAGCGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23710.24992963811326843No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22440.23654243269766945No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21890.23074482405311875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20540.21651433010740334No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC20210.2130357649206729No Hit
GCTTAATACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAACCTCTGGA20190.2128249427881438No Hit
GTATGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATT20130.21219247639055644No Hit
GTGTTAGTACCTACTTAGCCTGGTACCAAAAAAAACCTGGCCAGTCTCCC19880.20955719973394246No Hit
GGCTTAATACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAACCTCTGG19870.2094517886676779No Hit
GTATATTACTGTGTGAAAGATGGGCCCTACCATGGAGACTTCCGGATGGG19640.2070273341435931No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19380.20428664642071453No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18740.19754033817978278No Hit
CAGTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATC17850.18815875328223708No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17620.18573429875815223No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16900.17814470198710403No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16840.17751223558951665No Hit
GTCCCTACGCTGGTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGT16820.17730141345698755No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16790.17698518025819387No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16680.17582565852928372No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16180.1705551052160558No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16030.1689739392220874No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15810.16665489576426712No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAC15740.16591701830041522No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15300.16127893138477462No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTACCTACTTAGCCTGGTA15120.1593815321920126No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14640.15432180101131376No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14550.15337310141493277No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14410.1518973464872289No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14410.1518973464872289No Hit
GTCCCATACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTTTT14320.1509486468908479No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14220.1498945362282023No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14110.14873501449929216No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13970.14725925957158836No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13790.14536186037882629No Hit
GTTTATTACTGTCACCAGCGTAGGGACTGGCCAGGAGCGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAA13780.14525644931256174No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13400.1412508287945085No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAATGTGAGGTGCAGGTGGTGGAGT13350.1407237734631857No Hit
ATATTACTGTGTGAAAGATGGGCCCTACCATGGAGACTTCCGGATGGGAG13300.14019671813186294No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13270.13988048493306923No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13210.13924801853548188No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12950.13650733081260336No Hit
GTATTAAGCCTCCCCCAGACTCCACCACCTGCACCTCACATTGGACACCT12890.135874864415016No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG12690.13376664308972483No Hit
GACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTAGGGACTGG12690.13376664308972483No Hit
ACCATGGAGACTTCCGGATGGGAGGTTTTGATGTCTGGGGCCCAGGGACAATGGTCACCGTCTCTTCAGCATCCC12670.13355582095719573No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTACCTACTTAGCCTGGTACCAAAAAAAACCTGGCCAGTCTCCCAGGCT12350.13018266683672985No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTGGG12330.12997184470420073No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12300.12965561150540705No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12140.12796903444517413No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11960.12607163525241205No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11940.12586081311988295No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11850.12491211352350193No Hit
GGGTATGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAG11610.12238224793315253No Hit
CCCTGAGACTCTCCTGTGCAACCTCTGGATTCGCCTTTGGAACTTATTTC11570.12196060366809428No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTCGGTCCCTTGGCCGAACGCTCCTGGCCAGTC11570.12196060366809428No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11520.1214335483367715No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11440.12059025980665503No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCATACCCACTGCCA11320.11932532701148031No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11290.11900909381268665No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11250.11858744954762841No Hit
CTCTAGCCCCTTTCCTGGAGCCTGACGGACCCAGCTCATGAAATAAGTTC11230.11837662741509929No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC11190.11795498315004106No Hit
GGGCTAGAGTGGGTCTCACTTATTAGTGGTACTGGCGCTCAAATATACTA11130.1173225167524537No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11110.1171116946199246No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10960.11553052862595622No Hit
GTCTGGGGGAGGCTTAATACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAACCTCTGGATTCGCCTTTGGAA10870.11458182902957519No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10790.11373854049945872No Hit
CTCCCAGGCTCCTCATCTATGATACATCCAAAAGGGCCACTGGCATCCCA10540.11110326384284476No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10500.11068161957778651No Hit
GAGTGAAGTCTGTCCCATACCCACTGCCACTGAACCTGGCTGGGATGCCA10330.108889631451289No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10300.10857339825249535No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10290.1084679871862308No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA10270.10825716505370167No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10190.1074138765235852No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10160.10709764332479152No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10130.10678141012599786No Hit
CTCCTGTGCAACCTCTGGATTCGCCTTTGGAACTTATTTCATGAGCTGGG10070.10614894372841048No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT10020.10562188839708771No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTGGG9950.1048840109332358No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9770.10298661174047373No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG9730.1025649674754155No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9590.10108921254771168No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA9560.10077297934891802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)