FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985039
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36680.3723710431769707No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29560.3000896411208084No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCGAGTCAGGTGGCTGTCATAGGACTGG28810.2924757293873644No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27530.27948132002895315No Hit
CTCCTGCACTGGGAGCATCTCCAACATCGGGGCAGGTTATGATGTACACTGGTATCAGCAGCTTCCAGGAAGAGC26970.2737962659346483No Hit
GTAATAAACGGCCGTGTCCGCGGCAGTGACAGAGCTCAGCTTCAGGGAGAACTGGTTGTTAGATGCGTCTATAGA26290.2668929859629923No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCGAGTCAGGTGGCTGTCATAGGACTGGCAGTAATAAT25280.256639584828621No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22850.23197051081226225No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21970.22303685437835455No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20800.21115915207418184No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC20730.2104485203123937No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGTTTCTACTGGAGCTGGATCCG20230.20537257915676435No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19780.2008042321166979No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17980.18253084395643218No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16970.17227744282206087No Hit
GTTTATTACTGTGCGAGAGATCTCCGCGCACCAGGTGGTATGGGCTACTA16900.17156681106027274No Hit
GGTTATGATGTACACTGGTATCAGCAGCTTCCAGGAAGAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGG16820.17075466047537205No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16630.16882580283623289No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16470.1672015016664315No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16300.1654756816735175No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16120.1636483428574909No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15880.16121189110278883No Hit
GTCCTATGACAGCCACCTGACTCGGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGC15820.1606027781641133No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15310.15542531818537134No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15210.15441012995424547No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15170.15400405466179512No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15140.15369949819245737No Hit
GTCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAACC15010.15237975349199373No Hit
GATTATTACTGCCAGTCCTATGACAGCCACCTGACTCGGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT14730.14953722644484127No Hit
GCTCCAGACTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCC14250.14466432293543707No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14080.14293850294252308No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14070.14283698411941048No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14030.14243090882696016No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14020.14232939000384756No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAG13890.14100964530338392No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13680.1388777500180196No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTTCTACTGGA13580.1378625617868937No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13540.13745648649444336No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13280.1348169970935161No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13160.13359877121616504No Hit
GGCCATCACTGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCCAGT13020.13217750769258882No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12530.12720308536007205No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12230.12415752066669442No Hit
GTATCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGGAGCAT12170.1235484077280189No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11850.12029980538841609No Hit
GATGTACACTGGTATCAGCAGCTTCCAGGAAGAGCCCCCAAACTCCTCAT11850.12029980538841609No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11750.11928461715729022No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11660.11837094774927694No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11460.1163405712870252No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAACCAGAGAATCGGTC11370.1154269018790119No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11340.11512234540967414No Hit
CCTTGGTCCCTCCGCCGAAAACCCGAGTCAGGTGGCTGTCATAGGACTGG11310.11481778894033637No Hit
CAGTATCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGGAGC11190.11359956306298531No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11180.11349804423987273No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11100.11268589365497203No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCGAGTCAGGTGGC11090.11258437483185944No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10820.10984336660781958No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCT10790.10953881013848182No Hit
GTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGTGATGGCCAGGGAGG10730.1089296971998063No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10650.1081175466149056No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10590.10750843367623009No Hit
ACTACAACCCGTCCCTCAAGAGTCGGCTTTCCATGTCTATAGACGCATCT10470.10629020779887903No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10450.10608717015265386No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10340.10497046309841539No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10300.10456438780596504No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10260.1041583125135147No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9960.10111274782013707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGA200.007617237551.30001426
ACGACCG200.007641745751.25834717
TCGTATG1150.051.0492940
CGTATGC1250.047.00634441
TCTCGTA1100.046.9896838
TCTACAT1550.039.8558532
ATCTCGT1400.039.34155737
CCGTCTT1550.038.153247
ATGCCGT1550.038.03527544
ACGAGAC1550.037.48303222
GTATCAA21250.036.6908721
CACGAGA1600.036.3098421
TCTCCGA1650.035.2113311
CCCACGA1750.033.1958819
CGTCTTC1900.032.99507548
TATACAC1800.032.2852483
GCCCACG1800.032.27377718
TATCAAC24600.031.5441232
CGAGACC1850.031.40948323
GCCGTCT1900.031.08803246
Produced by FastQC (version 0.11.5)