FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985039
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35080.35612803147895666No Hit
GTATGTGATGACGCAGCCGCCCTCAGTATCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGGAGCAT29670.3012063481750469No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28320.28750130705484755No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27720.28141017766809234No Hit
CTCCTGCACTGGGAGCATCTCCAACATCGGGGCAGGTTATGATGTACACTGGTATCAGCAGCTTCCAGGAAGAGC25690.26080185657623706No Hit
GTAATAAACGGCCGTGTCCGCGGCAGTGACAGAGCTCAGCTTCAGGGAGAACTGGTTGTTAGATGCGTCTATAGA25620.26009122481444896No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCGAGTCAGGTGGCTGTCATAGGACTGG25050.2543046518970315No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23050.234000887274514No Hit
GTCCTGGGCCCAGTATGTGATGACGCAGCCGCCCTCAGTATCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTG22740.2308538037580238No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22410.2275036825953084No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCGAGTCAGGTGGCTGTCATAGGACTGGCAGTAATAAT21660.21988977086186434No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21330.21653964969914896No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGTTTCTACTGGAGCTGGATCCG20280.2058801732723273No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19200.19491614037616783No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18830.1911599439210021No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18220.18496729571113427No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC17570.17836857220881608No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17240.17501845104610073No Hit
GTTTATTACTGTGCGAGAGATCTCCGCGCACCAGGTGGTATGGGCTACTA16860.17116073576782237No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16410.16659238872775595No Hit
GGTTATGATGTACACTGGTATCAGCAGCTTCCAGGAAGAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGG16170.16415593697305386No Hit
GTCCTATGACAGCCACCTGACTCGGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGC15470.15704961935517273No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15350.15583139347782168No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15160.15390253583868252No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15030.15258279113821888No Hit
GATTATTACTGCCAGTCCTATGACAGCCACCTGACTCGGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT14850.1507554523221923No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14550.14770988762881468No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14500.14720229351325176No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14500.14720229351325176No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14350.14567951116656294No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14290.14507039822788742No Hit
GCTCCAGACTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCC14130.14344609705808603No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13900.14111116412649652No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13310.13512155356285385No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13270.1347154782704035No Hit
GTCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAACC13150.13349725239305246No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12870.1306547253459No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12680.12872586770676084No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12420.12608637830583358No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTTCTACTGGA12260.12446207713603219No Hit
GTATCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGGAGCAT12180.12364992655113148No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12100.12283777596623077No Hit
CTGCACAGGGTCCTGGGCCCAGTATGTGATGACGCAGCCGCCCTCAGTATCTGGGGCCCCAGGGCAGACGGTCAC12090.1227362571431182No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12020.12202562538133008No Hit
GATGTACACTGGTATCAGCAGCTTCCAGGAAGAGCCCCCAAACTCCTCAT11990.12172106891199233No Hit
GGCCATCACTGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCCAGT11790.11969069244974057No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11760.1193861359804028No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11730.11908157951106504No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAG11530.1170512030488133No Hit
CAGTATCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGGAGC11460.1163405712870252No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11460.1163405712870252No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11220.11390411953232309No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11100.11268589365497203No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10940.11106159248517064No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCT10900.11065551719272029No Hit
GCACAGGGTCCTGGGCCCAGTATGTGATGACGCAGCCGCCCTCAGTATCT10860.11024944190026993No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10800.10964032896159442No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10690.10852362190735595No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10340.10497046309841539No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10230.10385375604417693No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10140.10294008663616365No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10040.10192489840503778No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9990.10141730428947482No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAACCAGAGAATCGGTC9950.1010112289970245No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9860.10009755958901119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)