FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620951
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68710.4238869651210925No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64160.39581702346338665No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCC54830.3382582200202227No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51490.31765303207808254No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCATAGTAACTGG48310.2980349190074222No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44560.27490035170711513No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41180.254048395047105No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39710.2449796446653847No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39210.24189503569201043No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTTTGGGACAGAGTTCACT38760.23911888761597358No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38250.23597258646313185No Hit
GCATAGTAACTGGCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT38140.23529397248898948No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37850.23350489928443244No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37670.23239444005401766No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37410.23079044338786303No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37160.22924813890117593No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36330.22412768800537464No Hit
GTTTATTACTGTCAGCAGCATAGTAACTGGCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA35430.2185753918533009No Hit
GTAATATATGGCCGAGTCCGCCGCGGTCGCTGACTTCAGGTTCAGGGATA32650.20142496596134No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32400.19988266147465283No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC31360.19346667481003438No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30650.18908653006784287No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC30030.1852616149408588No Hit
GAGTGAACTCTGTCCCAAACCCACTGCCACTGAACCTGGCTGGGATACCA29300.18075808583973235No Hit
CTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCCACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCT29100.17952424225038266No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAAACCCACTGCCA28130.17354010084203655No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27880.17199779635534942No Hit
GTCTCCAGCCACCCTTTCTGTGTCTCCAGGAGAGGGGGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAG27700.1708873371249347No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT27620.1703937996891948No Hit
GTTACTATGCTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT27030.16675396110061316No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26900.16595196276753585No Hit
CCCTTTCTGTGTCTCCAGGAGAGGGGGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCCACTTAGC26580.1639778130245763No Hit
ATATATTACTGTGCGAGAGTTTCGCTGCTAAGCGATATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC26080.16089320405120203No Hit
GGGTGTTAGCAGCCACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATGCATC26080.16089320405120203No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25950.16009120571812474No Hit
CTCCAGGAGAGGGGGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGC25940.16002951353865724No Hit
GCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT25670.15836382469303514No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25310.15614290623220567No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24300.14991199610598963No Hit
GTCCCAAACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT24120.1488015368755749No Hit
TCTATAATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTTTGGGACAGAGTTCACT24030.14824630726036753No Hit
GGATACCAGTGGCCCTGGTGGATGCATTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGG23290.1436810859797736No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT22880.1411517066216067No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22560.13917755687864716No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22440.13843725072503735No Hit
GTCAGGGTGTTAGCAGCCACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATG22250.13726509931515513No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21620.13337849200870355No Hit
GTTTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT21610.13331679982923605No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21570.13307003111136612No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21520.13276157021402868No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21500.1326381858550937No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20970.12936850034331698No Hit
CCCCTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTTTCTGTGTCTCCAGGAGAGGGGGCCACCCTCTC20910.12899834726651205No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20810.1283814254718372No Hit
CATCTATAATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTTTGGGACAGAGTTCA20430.12603712265207276No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20270.12505004778059298No Hit
GACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATGCATCCACCAGGGCC20240.12486497124219054No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20160.12437143380645066No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20020.12350774329390585No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19880.12264405278136106No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19580.1207932873973365No Hit
GCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATGCATCCACCAGGGCCACTGGTATC19500.12029974996159662No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19460.12005298124372668No Hit
CTGCTACTCTGGCTCCCAGAGACCCCTGGAGAAATAGTGATGACGCAGTC19340.11931267509011687No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19170.1182639080391696No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19060.11758529406502725No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19050.11752360188555977No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18810.11604298957834011No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTTTCTGTGTCTCCAGG18790.11591960521940516No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18680.11524099124526281No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAAACCCACTGCCA18530.11431560855325053No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18300.11289668842549835No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18200.11227976663082352No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTTTGGGACAGAGTTCACTCTCACCATCAGCAG18200.11227976663082352No Hit
CACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATGCATCCACCAGGGCCA18070.11147776829774621No Hit
GTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCATAGTAACTGGCCTC17980.11092253868253883No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT17910.11049069342626643No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17820.10993546381105906No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17820.10993546381105906No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17790.1097503872726566No Hit
GGTCTCTGCTGGTACCAGGCTAAGTGGCTGCTAACACCCTGACTGGCCCTGCAGGAGAGGGTGGCCCCCTCTC17750.10950361855478667No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17720.1093185420163842No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCCCTGGAGAAATAG17440.10759116099129462No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCCCTGGAGAAATAGTGATGACGCAGTCTC17340.10697423919661976No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC17320.10685085483768479No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17280.10660408611981485No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17170.1059254721456725No Hit
GTTCAGTGGCAGTGGGTTTGGGACAGAGTTCACTCTCACCATCAGCAGCC16820.10376624586431052No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16740.10327270842857064No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16730.10321101624910314No Hit
GGCTCCCAGAGACCCCTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTTTCTGTGTCTCCAGGAGAGGGGG16650.10271747881336327No Hit
GGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTTTGGGACAGAGT16570.10222394137762339No Hit
CCTTGGTCCCTTGGCCGAACGTTCGAGGCCAGTTACTATGCTGCTGACAGTAATAAACTGCAAAATCTTCAGA16560.1021622491981559No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16220.10006471509626139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)