FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620951
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70650.43585524793778463No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55950.3451677441205811No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCC53130.32777054951075013No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52110.3214779472050666No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCATAGTAACTGG48170.29717122849487737No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42960.2650296029923174No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42090.2596623833786462No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39690.24485626030644972No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39680.24479456812698228No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39590.24423933851177487No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTTTGGGACAGAGTTCACT38740.2389955032570386No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38660.23850196582129876No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36490.2251147628768544No Hit
GCATAGTAACTGGCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT35840.22110477121146782No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34650.21376340185483705No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34650.21376340185483705No Hit
GTTTATTACTGTCAGCAGCATAGTAACTGGCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA34150.21067879288146277No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33940.2093832571126456No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32790.2022886564738848No Hit
GGGTGTTAGCAGCCACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATGCATC31530.1945154418609816No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31330.19328159827163188No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30120.18581684455606617No Hit
GTAATATATGGCCGAGTCCGCCGCGGTCGCTGACTTCAGGTTCAGGGATA30010.1851382305819238No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29990.18501484622298886No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC29320.18088147019866732No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC28870.17810532212263047No Hit
GAGTGAACTCTGTCCCAAACCCACTGCCACTGAACCTGGCTGGGATACCA28490.17576101930286603No Hit
CTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCCACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCT28020.1728614868678942No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAAACCCACTGCCA27900.1721211807142844No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT27600.17027041533025983No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27340.1686664186641052No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27320.16854303430517023No Hit
GTCTCCAGCCACCCTTTCTGTGTCTCCAGGAGAGGGGGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAG25880.15965936046185233No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25520.15743844200102286No Hit
ATATATTACTGTGCGAGAGTTTCGCTGCTAAGCGATATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC25010.1542921408481811No Hit
GTTACTATGCTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT24940.1538602955919087No Hit
CCCTTTCTGTGTCTCCAGGAGAGGGGGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCCACTTAGC24810.15305829725883138No Hit
GCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT24220.14941845867024975No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23630.1457786200816681No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23020.1420153971341515No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22630.13960940213491957No Hit
GTCCCAAACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT22390.1381287898276999No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT22250.13726509931515513No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22130.13652479316154528No Hit
GTTTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT22130.13652479316154528No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21400.13202126406041884No Hit
GTCAGGGTGTTAGCAGCCACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATG21220.1309108048300041No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21220.1309108048300041No Hit
CTCCAGGAGAGGGGGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGC21100.13017049867639427No Hit
GGATACCAGTGGCCCTGGTGGATGCATTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGG21040.1298003455995894No Hit
CCCCTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTTTCTGTGTCTCCAGGAGAGGGGGCCACCCTCTC20960.12930680816384948No Hit
TCTATAATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTTTGGGACAGAGTTCACT20810.1283814254718372No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20560.12683912098515007No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20460.12622219919047523No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20070.12381620419124328No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19960.12313759021710094No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19770.12196543880721872No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19460.12005298124372668No Hit
GACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATGCATCCACCAGGGCC19280.11894252201331194No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19010.11727683316768982No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18990.11715344880875486No Hit
CTGCTACTCTGGCTCCCAGAGACCCCTGGAGAAATAGTGATGACGCAGTC18810.11604298957834011No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18780.11585791303993767No Hit
CATCTATAATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTTTGGGACAGAGTTCA18760.11573452868100269No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18690.1153026834247303No Hit
GGTCTCTGCTGGTACCAGGCTAAGTGGCTGCTAACACCCTGACTGGCCCTGCAGGAGAGGGTGGCCCCCTCTC18640.11499422252739287No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18600.11474745380952293No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18510.11419222419431557No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18510.11419222419431557No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAAACCCACTGCCA18320.11302007278443335No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18290.11283499624603088No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18180.11215638227188854No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCCCTGGAGAAATAG17810.10987377163159157No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTTTCTGTGTCTCCAGG17550.10826977496543695No Hit
GCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATAATGCATCCACCAGGGCCACTGGTATC17320.10685085483768479No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17160.10586377996620502No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17100.1054936268894001No Hit
GTTCAGTGGCAGTGGGTTTGGGACAGAGTTCACTCTCACCATCAGCAGCC17060.10524685817153018No Hit
GTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCATAGTAACTGGCCTC17010.10493839727419274No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16710.10308763189016817No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16650.10271747881336327No Hit
CCTTGGTCCCTTGGCCGAACGTTCGAGGCCAGTTACTATGCTGCTGACAGTAATAAACTGCAAAATCTTCAGA16540.10203886483922092No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCCCTGGAGAAATAGTGATGACGCAGTCTC16520.10191548048028595No Hit
GGCTCCCAGAGACCCCTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTTTCTGTGTCTCCAGGAGAGGGGG16320.10068163689093625No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16300.10055825253200129No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16250.10024979163466385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA200.00788316450.8581168
GAATGAG11050.044.071170
GGTATCA16200.043.350331
GTGGTAT4150.036.78761
CGAATGA9650.034.69243669
AATGGTA4550.034.6716370
GTATCAA36750.033.510921
GACAGGT3000.030.5337091
ATCAACG42400.028.1478883
TCAACGC42600.027.9344274
AACGCAG43200.027.626636
TCGAATG11150.027.59562568
CAACGCA43400.027.4993215
TGTTAGA21500.027.43581270
GTCGAAT10500.027.37384267
TATCAAC43800.027.253232
GTGCTAG1750.027.1410731
GTCGTTC500.00842183427.1234939
TGTCGAA10750.026.36207466
ATACGTA1707.2759576E-1225.92606511
Produced by FastQC (version 0.11.5)