FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063290
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39930.3755325452134413No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39830.374592068015311No Hit
GTATAATGATTCCCCGGTGACGTTCGGCCAAGGGACCAAGGTGGAGATCGCACGAACTGTGGCTGCACCAT36500.3432741773175709No Hit
TCATTATACTGCTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGACTGAATTCTGTC35740.33612655061178043No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29060.2733026737766743No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25840.24301930799687763No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25010.23521334725239584No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24930.23446096549389162No Hit
CTATTAGTACCTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT23850.224303811754084No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23420.2202597598021236No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22740.21386451485483735No Hit
ATTAGTACCTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGCCTCCTGATCTATAAGGCGTCTAA22630.21282998993689398No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22480.2114192741396985No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21780.20483593375278616No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT21210.1994752137234433No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20610.1938323505346613No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20050.18856567822513143No Hit
CCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTACCTGG20030.18837758278550537No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18970.17840852448532385No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18950.1782204290456978No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18840.17718590412775442No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18760.17643352236925014No Hit
GACTATTAGTACCTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGCCTCCTGATCTATAAGGCGT18200.1711668500597203No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18040.16966208654271178No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16580.15593111945000893No Hit
GAATTCAGTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTTCTGCCAGCAGTATAATGATTCC16490.15508468997169164No Hit
GCCACAGTTCGTGCGATCTCCACCTTGGTCCCTTGGCCGAACGTCACCGG15750.14812515870552717No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15580.14652634746870563No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGCGATCTCCACCTTGGTCC15330.1441751544733798No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT15140.14238824779693215No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15100.14201205691768004No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14700.1382501481251587No Hit
GTGCAGCCACAGTTCGTGCGATCTCCACCTTGGTCCCTTGGCCGAACGTC14500.13636919372889802No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14390.13533466881095468No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14020.13185490317787243No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC13890.130632282820303No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13800.12978585334198572No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13720.12903347158348147No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTACCTGGCTGGCCTGGTATCAGCAGAAA13660.12846918526460327No Hit
GTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGAAGCAGACAGGGTGGAAGGAGA13170.12386084699376462No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13010.12235608347675611No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12870.12103941539937364No Hit
TCTATAAGGCGTCTAACTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAGT12830.1206632245201215No Hit
GTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA12770.12009893820124332No Hit
ATCTCCACCTTGGTCCCTTGGCCGAACGTCACCGGGGAATCATTATACTG12640.11887631784367388No Hit
GGACCAAGGTGGAGATCGCACGAACTGTGGCTGCACCATCTGTCTTCATC12380.11643107712853502No Hit
CCTTATAGATCAGGAGGCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAGCCAGGTACTAATAGTCTGA12190.1146441704520874No Hit
GTCTCCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAC12140.11417393185302223No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12070.11351559781433099No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12030.11313940693507886No Hit
GATCTATAAGGCGTCTAACTTAGAAAGTGGGGTCCCATCAAGGTTCAGCG12010.11295131149545279No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11710.1101298799010618No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11650.10956559358218361No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11630.10937749814255754No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11160.10495725531134498No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11150.10486320759153195No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11100.10439296899246678No Hit
CCTTGGTCCCTTGGCCGAACGTCACCGGGGAATCATTATACTGCTGGCAGAAATAAGTTGCAAAATCATCAGG10850.10204177599714094No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10840.10194772827732791No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10800.10157153739807577No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10780.10138344195844973No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10680.10044296476031937No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10660.10025486932069333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA200.00806538850.56373211
TACGTAT806.3520674E-541.4081470
CGTATGC1200.040.26466441
TCGTATG1150.038.92237540
GTATCAA26100.035.2656361
GACGTAG1550.034.8736326
AGACGTA1550.034.8489925
AATGGTA4700.033.8313370
CGTAGAG1700.033.82208628
CGAGACG1600.033.73136523
CCTAACG400.002906835433.7139132
ATGCCGT1450.033.53399344
TAGTCAC1550.033.14867838
CACGAGA1850.030.98763821
ATCTCGT1550.030.87973837
GAGACGT1750.030.84446124
TACCGAT3250.030.5783270
ATCTCCG1900.030.16082410
CTAACGC450.005174080429.9679243
AATCTCG1600.029.87200236
Produced by FastQC (version 0.11.5)