FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063290
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38970.36650396411139013No Hit
TCATTATACTGCTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGACTGAATTCTGTC36600.34421465451570127No Hit
GTATAATGATTCCCCGGTGACGTTCGGCCAAGGGACCAAGGTGGAGATCGCACGAACTGTGGCTGCACCAT35830.33697298009009774No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35680.3355622642929022No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28950.2722681488587309No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26210.24649907362995985No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24210.22768952966735323No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23240.218566900845489No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23160.21781451908698476No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22350.21019665378212904No Hit
CTATTAGTACCTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT21850.2054942677914774No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21370.2009799772404518No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20450.19232758701765276No Hit
ATTAGTACCTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGCCTCCTGATCTATAAGGCGTCTAA20270.19063472806101817No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19520.18358114907504067No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19510.18348710135522764No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18750.1763394746494371No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18740.1762454269296241No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18450.17351804305504615No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18160.17079065918046818No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17570.16524184371149922No Hit
CCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTACCTGG17440.1640192233539298No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16950.15941088508309117No Hit
GACTATTAGTACCTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGCCTCCTGATCTATAAGGCGT16930.1592227896434651No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16570.1558370717301959No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16400.15423826049337433No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16380.15405016505374827No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15720.14784301554608809No Hit
GAATTCAGTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTTCTGCCAGCAGTATAATGATTCC15330.1441751544733798No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGCGATCTCCACCTTGGTCC15190.14285848639599733No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14940.1405072934006715No Hit
GTGCAGCCACAGTTCGTGCGATCTCCACCTTGGTCCCTTGGCCGAACGTC14510.13646324144871108No Hit
GCCACAGTTCGTGCGATCTCCACCTTGGTCCCTTGGCCGAACGTCACCGG14260.13411204845338523No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT13760.1294096624627336No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13420.12621203998909047No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13410.12611799226927742No Hit
GTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGAAGCAGACAGGGTGGAAGGAGA12970.12197989259750397No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12790.12028703364086939No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12790.12028703364086939No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC12670.11915846100311299No Hit
GTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA12230.11502036133133951No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12110.11389178869358313No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12100.11379774097377009No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTACCTGGCTGGCCTGGTATCAGCAGAAA12050.11332750237470493No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12000.11285726377563976No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11970.11257512061620066No Hit
ATCTCCACCTTGGTCCCTTGGCCGAACGTCACCGGGGAATCATTATACTG11710.1101298799010618No Hit
GTCTCCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAC11630.10937749814255754No Hit
GATCTATAAGGCGTCTAACTTAGAAAGTGGGGTCCCATCAAGGTTCAGCG11610.10918940270293147No Hit
GGACCAAGGTGGAGATCGCACGAACTGTGGCTGCACCATCTGTCTTCATC11550.10862511638405326No Hit
TCTATAAGGCGTCTAACTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAGT11470.107872734625549No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11420.10740249602648384No Hit
GGTGGAGATCGCACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC11410.10730844830667081No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11290.10617987566891442No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11120.10458106443209285No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11040.10382868267358858No Hit
CCTTGGTCCCTTGGCCGAACGTCACCGGGGAATCATTATACTGCTGGCAGAAATAAGTTGCAAAATCATCAGG10980.10326439635471038No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10890.10241796687639308No Hit
CCTTATAGATCAGGAGGCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAGCCAGGTACTAATAGTCTGA10810.10166558511788883No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10700.10063106019994544No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10680.10044296476031937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3050.038.6170351
GGCGATC601.004108E-535.99337857
TTCGCAC400.00270437634.21403537
GTATCAA27350.034.2056771
TACGTAT1052.6707092E-432.43887370
CCGAAAC653.5961726E-430.8372368
GTTCTAC553.7684172E-430.5927181
GCTATAG450.005235227830.2762870
ATCAACG30900.029.823433
TCAACGC31050.029.6765634
TACCGAT4150.029.5467370
TATCAAC31500.029.4730662
CAACGCA31300.029.439535
AACGCAG31400.029.3457746
CAGTCGC605.848788E-428.37995534
GTACGTA955.1077885E-528.37012169
TAGTATC500.00703971628.13741349
CGCAGAG33500.027.50368
TCGCACT500.00798769227.41846738
ACGCAGA33750.027.3024377
Produced by FastQC (version 0.11.5)