FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997214
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTATACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA43790.43912339778623244No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC43100.4322041206802151No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGCTGTCACTCCACG35150.3524820148934933No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGGGACCC33800.33894429881650273No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGG27770.27847583367261186No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27210.27286018848511956No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT26480.26553979386570986No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26050.2612277805967425No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25120.2519017984103713No Hit
GTATACTACTGTGCGAAAGGGCAGGTCTGGCGCGATTACTATTACTATTT24060.24127218430547506No Hit
GATTATTATTGTGCAGCGTGGAGTGACAGCCTGAATGGCTGGGTGTTCGG23550.2361579360097231No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGGTCCCTTCA22480.22542804252647877No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22090.22151714677090376No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21390.2144975902865383No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21120.2117900470711402No Hit
GTCTTCTCCACGGTGGTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20270.20326629991155357No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20120.20176210923633242No Hit
ATAATAATCAGCCTCATCCTCAGACTGCAGCCCACTGATGGCCAGGGAGG20050.20106015358789586No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCAGCGTGGAGTGACAGCCTGAATGGCTGGGTGTTCGGCGGAGGGAC20010.20065903607450358No Hit
GTGCTGACTCAGCCACCCTCAACGTCTGGGACCCCCGGGCAGAGGGTCAC19740.19795149285910546No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19730.1978512134807574No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTGCAGT19000.1905308188613477No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18140.18190679232341303No Hit
TCCTCAGACTGCAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC18100.18150567481002072No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17930.17980092537810338No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17560.17609058837922453No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17410.17458639770400336No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17030.1707757813267764No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17020.17067550194842832No Hit
CCCCTGAGGGCCGCCGATTATTACTATAGATGAGGAGTTTGGGGGCCGTT16970.17017410505668792No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16960.17007382567833987No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16560.16606265054441677No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16100.16144979914040516No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15840.15884253530335515No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15310.15352772825090702No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15250.15292605198081857No Hit
GCCGTATACTACTGTGCGAAAGGGCAGGTCTGGCGCGATTACTATTACTA15080.15122130254890126No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGCAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG14890.14931599436028778No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGC14790.148313200576807No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14370.1441014666861877No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14120.14159448222748577No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT13210.1324690587978107No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13200.13236877941946262No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13190.13226850004111454No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13090.13126570625763376No Hit
GCACAATAATAATCAGCCTCATCCTCAGACTGCAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG13070.13106514750093762No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12890.12926011869067222No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12850.12885900117727989No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12710.12745508988040682No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12420.12454698790831256No Hit
GCCACATACCGCTCATCCGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCAAGAACACG12370.12404559101657217No Hit
TCCGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCAA12130.12163888593621831No Hit
GGCCATCAGTGGGCTGCAGTCTGAGGATGAGGCTGATTATTATTGTGCAG12070.12103720966612985No Hit
GTATTAGTGGGAGTGGTGGTGCCACATACCGCTCATCCGCAGACTCCGTG11910.11943273961256058No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11880.11913190147751637No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11810.11842994582907981No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11740.11772799018064327No Hit
TTATTGTGCAGCGTGGAGTGACAGCCTGAATGGCTGGGTGTTCGGCGGAG11690.11722659328890289No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11580.11612352012707404No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11580.11612352012707404No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11560.11592296137037787No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG11530.11562212323533365No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11470.11502044696524516No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC11350.11381709442506824No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTGCAGTCTGAGGATG11170.11201206561480283No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11160.11191178623645476No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC10890.10920424302105668No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10870.1090036842643605No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT10860.10890340488601243No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGTAGGTAATACTATAAATTGGTATCAGCAGGTCCCAGGAACGGCCCC10700.1072989348324432No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGGTCCCTTCATGCGTGACCTGG10540.10569446477887395No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC10500.10529334726548163No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10420.104491112238697No Hit
GTATACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGCG10360.10388943596860854No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10360.10388943596860854No Hit
CCCTCAACGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG10300.10328775969852008No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10260.10288664218512777No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10170.10198412777999506No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10120.10148273088825467No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10100.10128217213155853No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10030.10058021648312197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)