FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997214
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC41100.4121482450105995No Hit
GTAGTATACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA39960.4007163958789186No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGGGACCC35140.3523817355151452No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGCTGTCACTCCACG33490.33583563808771233No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29400.2948213723433486No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT26010.26082666308335023No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGG25190.2526037540588078No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23620.23685989165815963No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23140.23204648149745188No Hit
GATTATTATTGTGCAGCGTGGAGTGACAGCCTGAATGGCTGGGTGTTCGG22090.22151714677090376No Hit
GTATACTACTGTGCGAAAGGGCAGGTCTGGCGCGATTACTATTACTATTT21990.22051435298742297No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21530.21590150158341137No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21290.21349479650305753No Hit
GTCTTCTCCACGGTGGTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20980.21038613577426712No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCAGCGTGGAGTGACAGCCTGAATGGCTGGGTGTTCGGCGGAGGGAC20890.20948362136913443No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20510.20567300499190747No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGGTCCCTTCA20440.2049710493434709No Hit
GTGCTGACTCAGCCACCCTCAACGTCTGGGACCCCCGGGCAGAGGGTCAC20140.2019626679930286No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20010.20065903607450358No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19840.19895428664258624No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19450.1950433908870112No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18630.18682048186246886No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17980.18030232226984377No Hit
ATAATAATCAGCCTCATCCTCAGACTGCAGCCCACTGATGGCCAGGGAGG17640.17689282340600912No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTGCAGT16790.16836907624642253No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16440.16485929800423982No Hit
CCCCTGAGGGCCGCCGATTATTACTATAGATGAGGAGTTTGGGGGCCGTT16150.16195119603214556No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15890.15934393219509554No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15740.15783974151987437No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15620.15663638897969745No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15560.15603471270960897No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15530.15573387457456475No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15260.15302633135916663No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGCAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG15020.15061962627881276No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14890.14931599436028778No Hit
TCCTCAGACTGCAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC14720.14761124492837044No Hit
GCCGTATACTACTGTGCGAAAGGGCAGGTCTGGCGCGATTACTATTACTA14260.14299839352435886No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14190.14229643787592233No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC14170.14209587911922616No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGC13940.13978945341722038No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13640.13678107206677803No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13620.1365805133100819No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13400.1343743669864242No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT12950.12986179496076067No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12850.12885900117727989No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12830.12865844242058375No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12780.12815704552884336No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12740.12775592801545105No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12570.12605117858353374No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12400.1243464291516164No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG12330.12364447350317986No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12270.1230427972330914No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12160.12193972407126252No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12090.12123776842282599No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11960.11993413650430097No Hit
GGCCATCAGTGGGCTGCAGTCTGAGGATGAGGCTGATTATTATTGTGCAG11870.11903162209916829No Hit
GCCACATACCGCTCATCCGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCAAGAACACG11730.1176277108022952No Hit
GCACAATAATAATCAGCCTCATCCTCAGACTGCAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG11680.11712631391055481No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11310.11341597691167593No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGTAGGTAATACTATAAATTGGTATCAGCAGGTCCCAGGAACGGCCCC11070.11100927183132206No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11040.11070843369627784No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10920.10950508115610089No Hit
GTATTAGTGGGAGTGGTGGTGCCACATACCGCTCATCCGCAGACTCCGTG10810.10840200799427203No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10730.1075997729674874No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC10700.1072989348324432No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10680.10709837607574701No Hit
GTATACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGCG10510.10539362664382972No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA10320.10348831845521624No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10220.10248552467173545No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTGCAGTCTGAGGATG10200.1022849659150393No Hit
CCCTCAACGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG10180.10208440715834313No Hit
TCCGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCAA10150.10178356902329892No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACTTTTAGCAACTATGCCATGAGCTGGGTCCG10140.10168328964495083No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT10090.10118189275321043No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10060.10088105461816621No Hit
TTATTGTGCAGCGTGGAGTGACAGCCTGAATGGCTGGGTGTTCGGCGGAG10060.10088105461816621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)