FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201984
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGGCACAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC44910.3736322613279378No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43910.3653126830307225No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC40290.3351958095948032No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35990.2994216229167776No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34550.2874414301687876No Hit
GTCCTGGGCCCAGTCTGTGTTGGCACAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG34100.2836976199350407No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG33930.28228329162451415No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC32000.26622650551088867No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29630.24650910494648848No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGG29120.2422661200149087No Hit
GATTATTACTGCGTAACATGGGATAGCAGCCTGAGTGGTGTGGTATTCGG26640.22163356583781482No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26110.21722418934029072No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAGTCAGGTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAG25850.21506109898301473No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25440.21165007188115648No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT24740.20582636707310578No Hit
CCCAGGAACAGCCCCCAAACTCCTCATCTATGAAAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG24440.2033304935839412No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23510.19559328576753102No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23500.19551008998455888No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22900.1905183430062297No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGTTTTGTTTGGAGGGTGTGGTGGTCTCCA22170.18444505084926255No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21880.1820323731430701No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21230.1766246472498802No Hit
ATACCACACCACTCAGGCTGCTATCCCATGTTACGCAGTAATAATCGGCC20990.17462794845854854No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20730.17246485810127257No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20720.1723816623183004No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGTTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20580.17121692135669028No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20410.16980259304616366No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19980.1662251743783611No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19920.1657259996805282No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC19380.16123342740003194No Hit
ACATGGGATAGCAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG19040.15840477077897877No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT18440.15341302380064958No Hit
CCACCACACCCTCCAAACAAAACAACAACAAGTACGCGGCCAGCAGCTAT17590.14634138224801663No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG17540.14592540333315585No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17430.14501024972046217No Hit
ATCCCATGTTACGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG17310.14401190032479635No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17240.14342952984399127No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17140.14259757201426973No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG16940.1409336563548267No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16390.13635788829135828No Hit
GGATAGCAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC16240.135109951546776No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGGCACAGCCGCCCTCAGTGTCT16220.13494355998083168No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGTAA15770.13119974974708482No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT15570.12953583408764177No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGGC15040.12512645759011767No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14750.12271377988392525No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14740.1226305841009531No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14680.12213140940312017No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14660.12196501783717587No Hit
CTCCTGCTCTGGAGTCAGGTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCC14480.12046749374367711No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGTCAGACTTGGAGCC14440.1201347106117885No Hit
GCCCCCAAACTCCTCATCTATGAAAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT14160.11780522868856823No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14090.11722285820776318No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC13990.11639090037804165No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT13790.11472698471859859No Hit
GTTCCTGGGAGCTGCTGGTACCAGGATACATAATTATTCCCAATGTTGGA13710.11406141845482136No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13670.11372863532293274No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG13520.11248069857835047No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13110.10906967147649221No Hit
CTCCTCATCTATGAAAATAATAAGCGACCCTCAGGGATTCCTGACCGATT13080.10882008412757575No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC12920.1074889516000213No Hit
GCTCTGGAGTCAGGTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAA12720.10582503594057824No Hit
GCTTGGTCCCTCCGCCGAATACCACACCACTCAGGCTGCTATCCCATGTTACGCAGTAATAATCGGCCTCGTCCC12650.10524266545977316No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12640.10515946967680101No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12530.10424431606410735No Hit
CCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGC12520.1041611202811352No Hit
CCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAG12470.10374514136627443No Hit
GGGTCCTGGGCCCAGTCTGTGTTGGCACAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTC12320.10249720462169214No Hit
GTCTGTGTTGGCACAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGG12320.10249720462169214No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12190.10141565944305414No Hit
GGTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGA12140.10099968052819337No Hit
GATGAGGAGTTTGGGGGCTGTTCCTGGGAGCTGCTGGTACCAGGATACATAATTATTCCCAATGTTGGACCTGA12020.10000133113252756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)