FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201984
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50610.4210538576220648No Hit
GTGTTGGCACAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC45250.376460917948991No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44080.36672701134124913No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC39240.3264602523827272No Hit
GTCCTGGGCCCAGTCTGTGTTGGCACAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG38320.31880624034928917No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37750.31406408071987646No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33570.2792882434375166No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG31280.26023640913689366No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29100.24209972844896438No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27710.23053551461583519No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27410.22803964112667058No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGG25560.21264842127682232No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25030.20823904477929822No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24650.20507760502635644No Hit
GATTATTACTGCGTAACATGGGATAGCAGCCTGAGTGGTGTGGTATTCGG24340.20249853575421967No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAGTCAGGTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAG24310.20224894840530322No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24220.20150018635855388No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22850.19010236409136894No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21030.17496073159043715No Hit
CCCAGGAACAGCCCCCAAACTCCTCATCTATGAAAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG21020.17487753580746498No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGTTTTGTTTGGAGGGTGTGGTGGTCTCCA20920.17404557797774345No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC20900.17387918641179914No Hit
ATACCACACCACTCAGGCTGCTATCCCATGTTACGCAGTAATAATCGGCC20790.1729640327991055No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20310.16897063521644215No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGTTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19810.16481084606783453No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19730.1641452798040573No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19380.16123342740003194No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19300.16056786113625474No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT18820.1565744635535914No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18650.1551601352430648No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18180.15124993344337362No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG18050.15016838826473564No Hit
ACATGGGATAGCAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG17810.14817168947340398No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17070.14201520153346467No Hit
ATCCCATGTTACGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG16140.13427799371705446No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15960.13278046962355572No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15920.1324476864916671No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT15850.13186531601086204No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15810.13153253287897343No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG15630.13003500878547467No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15630.13003500878547467No Hit
GGATAGCAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC15480.1287870720408924No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14940.12429449976039614No Hit
CCACCACACCCTCCAAACAAAACAACAACAAGTACGCGGCCAGCAGCTAT14790.12304656301581386No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14700.12229780096906448No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14650.12188182205420371No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGGCACAGCCGCCCTCAGTGTCT14630.1217154304882594No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14420.1199683190458442No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC14390.11971873169692773No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGTAA14380.11963553591395558No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGTCAGACTTGGAGCC14360.1194691443480113No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14330.11921955699909484No Hit
CTCCTGCTCTGGAGTCAGGTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCC14110.11738924977370749No Hit
CTCCTCATCTATGAAAATAATAAGCGACCCTCAGGGATTCCTGACCGATT13960.11614131302912517No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT13630.11339585219104413No Hit
GCCCCCAAACTCCTCATCTATGAAAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT13610.11322946062509985No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13540.11264709014429478No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG13520.11248069857835047No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13520.11248069857835047No Hit
GCTCTGGAGTCAGGTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAA13380.11131595761674032No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13190.10973523774026941No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGGC12970.10790493051488206No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC12720.10582503594057824No Hit
CCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAG12620.1049930781108567No Hit
GTTTGGGGGCTGTTCCTGGGAGCTGCTGGTACCAGGATACATAATTATTC12400.10316277088546935No Hit
GGGTCCTGGGCCCAGTCTGTGTTGGCACAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTC12380.10299637931952504No Hit
GCTTGGTCCCTCCGCCGAATACCACACCACTCAGGCTGCTATCCCATGTTACGCAGTAATAATCGGCCTCGTCCC12370.10291318353655289No Hit
CTGCTATCCCATGTTACGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAG12280.10216442148980352No Hit
GTTCCTGGGAGCTGCTGGTACCAGGATACATAATTATTCCCAATGTTGGA12220.1016652467919706No Hit
GGTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGA12170.10124926787710983No Hit
GTCTGTGTTGGCACAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGG12170.10124926787710983No Hit
GAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCC12090.10058370161333263No Hit
GGGTGGCTGACGTGTCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCT12050.10025091848144402No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12030.10008452691549971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT200.00788935950.8472069
GGTATCA14150.040.5147671
CGCCTAA400.002364243635.1650650
GTATCAA33850.034.4732971
AATGGTA5200.033.14389870
TATCAAC38350.030.2462462
TATGCGG450.00503727230.1329295
ATCAACG39550.029.0591743
TCAACGC39800.028.8742374
CAACGCA40450.028.4940575
AACGCAG40600.028.3887836
TGGTATC4550.027.5804352
CGCAGAG42550.027.0877698
ACGCAGA42600.027.0559757
AGAGTAC42250.027.03715111
CAGAGTA42900.026.70762410
GGATGGG6250.026.51511670
GCAGAGT43700.026.2962679
GTACCCG659.347442E-426.19208330
AGCATCG659.591737E-426.0765747
Produced by FastQC (version 0.11.5)