FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075343
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGGTCCAACAATGGGGACTACTTAGCTTGGTACCAGCACAA64300.5979487475159089No Hit
CTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAATCCGGGGTCTCT52850.49147109340926565No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50360.4683156909004848No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44720.41586730931433036No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAA39240.3649068250781378No Hit
GTTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTCCCCATTGTTGGACCTGTATAAAAGACT36090.33561384600076444No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34590.32166480834487227No Hit
GTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAA33070.30752978352023497No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32120.2986953930048366No Hit
GTTTATTACTGTCAACAATGTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAA31150.289675015320693No Hit
CTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCA29820.27730686859913534No Hit
GTATAAAAGACTCTGATTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA29090.27051833693993454No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28880.2685654716681096No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28170.261962927177654No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27160.25257057515602No Hit
GTACTAAAACATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT26430.24578204349681915No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25500.23713364015016605No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25220.23452981978773282No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25110.2335068903596341No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24780.2304381020753378No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24320.22616039719419756No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23650.21992982704123243No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22510.2093285584227544No Hit
GGCCAAAAGTGTACGGAGTACTAAAACATTGTTGACAGTAATAAACTGCC22450.20877059691651872No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21910.20374894336039756No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA21560.20049416790735605No Hit
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA21080.1960304758574706No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20430.18998589287325068No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATGTTTTAGTACT20100.1869171045889544No Hit
CTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAATC20050.18645213666709132No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCC20020.1861731559139735No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18570.17268908617994447No Hit
GGTCCAACAATGGGGACTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTTCCTGGG18480.17185214392059092No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAATCAGAGTCTTTTATACAGGTCCAA18370.17082921449249217No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18200.16924832355815772No Hit
ACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATT18150.16878335563629465No Hit
GTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTCCCCATTGTTGGACCTG18140.16869036205192203No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAATCAGAGTCTTTTATA18050.16785341979256851No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAAT17930.16673749678009714No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17450.16227380473021166No Hit
GTGCAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTA17410.1619018303927212No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCCCCTGGCCAAAAGTG17300.16087890096462246No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17250.1604139330427594No Hit
GTCTCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16980.15790310626469878No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16860.15678718325222743No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16830.15650820249910957No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16690.15520629231789299No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16260.15120756818987058No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15870.14758081839933865No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15740.14637190180249463No Hit
GCACAAACCAGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGACCGATT15600.14506999162127804No Hit
GTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGA15350.1427451520119627No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15180.14116426107762825No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14840.13800247920895936No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC14400.13391076149656433No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14340.13335279999032867No Hit
GTCAGAGACCCCGGATTCCCGGGCAGATGCCCAGGAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTGTGCTGGTA14170.13177190905599423No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14120.13130694113413116No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13890.12916808869356103No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13840.12870312077169796No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13780.12814515926546227No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13690.12730821700610875No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13660.1270292362529909No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13380.12442541589055771No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13370.1243324223061851No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13310.12377446079994942No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13200.12275153137185064No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12970.12061267893128053No Hit
ATTTATTACTGTGCGAGAGATGTCCCCTCAGTGGGAGCAGCTGACTACTGGGGCCAGGGAACCCAGGTCACCGT12890.11986873025629961No Hit
GTCCTGTGCAGCCTCTGGATTCACCTTTAGTAATTTTTACATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG12880.119775736671927No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12780.11884580082820087No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12620.11735790347823905No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12590.1170789227251212No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12500.11624198046576767No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12460.11587000612827722No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAAC12460.11587000612827722No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12360.11494007028455105No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12360.11494007028455105No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12330.11466108953143321No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAATCAGAGTCTTTTATACAGGTCCAACAATGGGGACTA12300.11438210877831537No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12130.11280121784398095No Hit
GCTCATTTCCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT11950.11112733332527389No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11940.11103433974090128No Hit
GCCCTGCACAGAGCCCACGTAATATTTGCCACTTCCATCTTGGCTTATCTTGGCCAACCACTCCAGCCCCTTCC11920.11084835257215604No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11640.10824453220972284No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG11590.10777956428785979No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11480.10675663485976103No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11450.10647765410664319No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11440.10638466052227058No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11390.1059196926004075No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11380.10582669901603489No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11280.10489676317230875No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11240.10452478883481828No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11230.10443179525044567No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11220.10433880166607307No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11210.10424580808170043No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11190.10405982091295522No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11180.10396682732858259No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11060.10285090431611124No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10950.10182797488801247No Hit
TTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGA10940.10173498130363987No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT10780.10024708395367803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA150.002435583268.4206853
TCGTATG2750.047.73196840
CGGTGTT305.799826E-446.79548355
TAGCGCT2850.044.5065729
CGTATGC2800.044.44774641
ACGAGAC3200.043.85647222
CTAGCGC2900.043.7269928
GGTATCA8700.043.2685431
ATCTCGT2950.043.19919237
CTTATAC3250.042.1187481
CACGAGA3350.041.8908121
CGCTCAT3050.041.6599432
ATGCCGT3000.041.60774644
CATCTCG3000.041.30211336
CGAGACT3250.041.07915523
CCGTCTT3150.040.83880647
GCCGTCT3150.040.8137346
TATACAC3450.040.655773
GAGACTA3300.040.46427524
ACTAGCG3150.040.23971627
Produced by FastQC (version 0.11.5)