FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075343
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGGTCCAACAATGGGGACTACTTAGCTTGGTACCAGCACAA52130.4847755553344375No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51100.47519721614405824No Hit
CTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAATCCGGGGTCTCT46870.4358609299544424No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37310.3469590632942233No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36170.33635779467574534No Hit
GTTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTCCCCATTGTTGGACCTGTATAAAAGACT33280.30948264879205983No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAA32560.30278711071723163No Hit
GTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAA32360.30092723902977936No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA31020.28846609872384904No Hit
GTACTAAAACATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT30850.2868852077895146No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30300.2817705606490208No Hit
GTTTATTACTGTCAACAATGTTTTAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAA29920.2782368044428615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28400.2641017796182242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28300.263171843774498No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27080.25182662648103904No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26940.2505247162998225No Hit
CTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCA26880.2499667547935868No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25030.23276294168465317No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24500.22783428171290462No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24260.2256024356879619No Hit
GGCCAAAAGTGTACGGAGTACTAAAACATTGTTGACAGTAATAAACTGCC23570.21918587836625153No Hit
GTATAAAAGACTCTGATTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA22840.21239734670705068No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22540.20960753917587224No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22520.20942155200712703No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21350.19854130263553116No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21050.19575149510435275No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20730.1927757004044291No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19950.1855222008233652No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19840.18449927139526645No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATGTTTTAGTACT18700.17389800277678844No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCCCCTGGCCAAAAGTG18300.17017825940188386No Hit
GTCCTGTGCAGCCTCTGGATTCACCTTTAGTAATTTTTACATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG18020.16757443903945068No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCC17870.16617953527386145No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17720.16478463150827224No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17000.15808909343344402No Hit
GTGCAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTA16830.15650820249910957No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAATCAGAGTCTTTTATACAGGTCCAA16610.15446234364291206No Hit
GTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTCCCCATTGTTGGACCTG16480.1532534270460681No Hit
GTCTCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16470.15316043346169547No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16330.15185852328047889No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAATCAGAGTCTTTTATA16060.1493476965024183No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAAT16010.14888272858055523No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGAGACCCCG15980.14860374782743738No Hit
GGTCCAACAATGGGGACTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTTCCTGGG15810.14702285689310296No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15770.14665088255561248No Hit
CTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAATC15580.14488400445253283No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15280.14209419692135442No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15120.14060629957139256No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGAGACCCCGGATTCCCGGGC15030.13976935731203904No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14810.13772349845584153No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14610.13586362676838926No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGAGACC14500.13484069734029047No Hit
ACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATT14410.13400375508093695No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14400.13391076149656433No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14320.13316681282158344No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14170.13177190905599423No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14020.13037700529040502No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13950.12972605019979672No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13830.12861012718732534No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13820.12851713360295272No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13750.12786617851234444No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13670.12712222983736352No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13640.12684324908424569No Hit
GTCAGAGACCCCGGATTCCCGGGCAGATGCCCAGGAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTGTGCTGGTA13610.12656426833112785No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13460.12516936456553862No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC13210.12284452495622328No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13080.1216356083593793No Hit
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA12980.12070567251565313No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12830.11931076875006394No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12810.1191247815813187No Hit
GCACAAACCAGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGACCGATT12640.11754389064698426No Hit
ATTTATTACTGTGCGAGAGATGTCCCCTCAGTGGGAGCAGCTGACTACTGGGGCCAGGGAACCCAGGTCACCGT12570.11689293555637596No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12530.11652096121888551No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12520.11642796763451291No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12420.11549803179078674No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12140.11289421142835356No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12010.11168529483150957No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG11960.11122032690964652No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11940.11103433974090128No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAAC11910.11075535898778344No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11850.11019739748154775No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11770.10945344880656682No Hit
GTACCAGCACAAACCAGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAATCCGGGGTCTCTGA11750.10926746163782161No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11520.10712860919725148No Hit
GTGTACGGAGTACTAAAACATTGTTGACAGTAATAAACTGCCACATCTTC11440.10638466052227058No Hit
CCCTGAGACTGTCCTGTGCAGCCTCTGGATTCACCTTTAGTAATTTTTAC11400.1060126861847801No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11320.1052687375097992No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11300.10508275034105397No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT11210.10424580808170043No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11100.10322287865360169No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10990.10219994922550293No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10970.10201396205675771No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10960.10192096847238509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTTT200.007167937752.09522245
GCGGCTA200.00743016851.6239836
GGTATCA10100.046.3485371
GTGGTAT2600.040.740391
GTATCAA25350.038.145671
GCGGAAC400.002704446434.21393625
TGGTATC2850.033.554282
GACAGGT1850.033.245861
AGAGCCG9500.032.81114270
TATCAAC30150.032.057812
ATCAACG30450.031.5132523
TCAACGC30600.031.2471774
CAACGCA31000.030.8425545
AACGCAG31300.030.5469386
ATACTAC450.004859852630.3544033
GGTCTGG20900.029.1936770
CGCAGAG32900.029.0613758
ACGCAGA33150.028.8422057
AGAGTAC32800.028.62810511
GCAGAGT33350.028.4644589
Produced by FastQC (version 0.11.5)