FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749194
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA74160.42396669551805No Hit
GTGTATTACTGTGCGAGAGACGAGACTGTAGTGATACCAGCAGACTACTT54090.30922813593003406No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC50100.28641763006276033No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT45650.26097734156417185No Hit
CCATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT45370.2593766043103281No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43060.24617052196611697No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39780.22741902842108994No Hit
AGCCAGGACAGGCCCCTGTATTTGTCATCTATGGTAAAACCAACCGGCCC37790.2160423600812717No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35010.20014932591810858No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34990.20003498754283403No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31980.1828270620640135No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA30810.17613826711045202No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29940.17116454778600887No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC28110.16070258644838709No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27230.15567169793630667No Hit
CTCCTGTGCAGCCTCCGCATTCACCTTCAGTAGTTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG27100.15492849849702206No Hit
GGTCAGCACTGCGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG26940.1540137914948256No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26670.15247022342861913No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA26550.15178419317697178No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA26440.15115533211296173No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTCACCATTATGTCTTCGGAA26260.15012628673549075No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGTGACCA25610.1464102895390677No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25590.14629595116379315No Hit
GTCCTGGCTTCTGCTGGTACCAGTTTGCATAATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT25390.1451525674110476No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25160.14383767609539022No Hit
GTATTACTGTGCGAGAGACGAGACTGTAGTGATACCAGCAGACTACTTCT24590.14057903240006542No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24380.13937847945968257No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23620.1350336211992495No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGTGACCAC23540.13457626769815126No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23440.13400457582177847No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGCCCCTGCT23440.13400457582177847No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23130.13223233100502288No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGCCCCTGCTGTGTCTGTGGCCTTGGGA22960.13126045481518916No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22630.129373871623159No Hit
GGACAGCAGTGGTCACCATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC22340.12771596518167797No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTCACCATTATGTCTTCGG22210.12697276574239336No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22180.1268012581794815No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22040.12600088955255964No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA21600.12348544529651943No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT21460.12268507666959753No Hit
CTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGC21340.12199904641795022No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21300.1217703696674011No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20890.11942643297427273No Hit
ACATAATGGTGACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTC20600.11776852653279167No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20600.11776852653279167No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGTGAC20550.11748268059460529No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20470.11702532709350706No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20370.1164536352171343No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20210.11553892821493784No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG20190.1154245898396633No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19930.11393819096109409No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGCTATAACTAC19830.11336649908472131No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19700.1126232996454367No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19480.1113655775174166No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19460.11125123914214204No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19440.11113690076686748No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19420.11102256239159292No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAACTGGTA19350.110622378078132No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGC18810.107535241945719No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18700.10690638088170895No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT18610.10639185819297345No Hit
CCCCTGTATTTGTCATCTATGGTAAAACCAACCGGCCCTCAGGGATCCCA18600.10633468900533617No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG18510.10582016631660068No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTTG18470.10559148956605156No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18230.10421942906275691No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18070.10330472206056046No Hit
GTATTTGTCATCTATGGTAAAACCAACCGGCCCTCAGGGATCCCAGACCG17960.1026758609965504No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGGTCAGCACTGCGC17910.10239001505836402No Hit
GGACAGGCCCCTGTATTTGTCATCTATGGTAAAACCAACCGGCCCTCAGG17910.10239001505836402No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT17560.1003890934910593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT200.00736042451.7486341
CGTATGC3000.042.54887441
TCTCGTA2050.041.87998638
TCGTATG3000.041.3377140
TCTGGAG25100.041.2144670
GAGCGAT3500.040.9613632
ATCTCGT2200.038.96823537
AGGGGGG4650.038.50432670
GCCGTCT3200.038.019246
TATACAC3950.037.850363
GTCGTAT1100.037.5307939
GTATGCC3700.037.35377542
CGAGACA4200.036.41055723
ATGTCGT1250.035.66372737
ATCTCCG4100.035.63580710
GATCTCG2500.035.6308336
CCCACGA4050.035.23376519
CACGAGA4150.035.20746621
ATGCCGT3750.035.11604744
TTATACA4200.034.788332
Produced by FastQC (version 0.11.5)