FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749194
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA64070.366282985192037No Hit
GTGTATTACTGTGCGAGAGACGAGACTGTAGTGATACCAGCAGACTACTT54630.312315272062447No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC48830.2791571432328261No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC43260.2473139057188625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42850.24496996902573415No Hit
CCATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT42700.24411243121117499No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT41580.23770948219579988No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA39470.22564678360433432No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33860.19357486933982165No Hit
AGCCAGGACAGGCCCCTGTATTTGTCATCTATGGTAAAACCAACCGGCCC33770.19306034665108615No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG31540.18031161780797328No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31480.1799686026821496No Hit
CTCCTGTGCAGCCTCCGCATTCACCTTCAGTAGTTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG27620.1579012962541605No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC27320.15618622062504217No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC27110.15498566768465935No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA27070.15475699093411022No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27010.15441397580828656No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26290.1502977942984026No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26120.14932591810856885No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25850.14778235004236237No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTCACCATTATGTCTTCGGAA24860.1421226004662719No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24850.14206543127863464No Hit
GTATTACTGTGCGAGAGACGAGACTGTAGTGATACCAGCAGACTACTTCT24690.14115072427643818No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24660.14097921671352634No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGCCCCTGCTGTGTCTGTGGCCTTGGGA24550.14035035564951628No Hit
GGTCAGCACTGCGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG24400.13949281783495715No Hit
GTCCTGGCTTCTGCTGGTACCAGTTTGCATAATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT24130.13794924976875064No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23820.13617700495199503No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23770.13589115901380863No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA23520.1344619293228767No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGCCCCTGCT23030.13166063912865011No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22660.12954537918607084No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22600.12920236406024715No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGTGACCA22600.12920236406024715No Hit
CTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGC22120.12645824305365785No Hit
GGACAGCAGTGGTCACCATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC22090.126286735490746No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21940.12542919767618688No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21590.12342827610888214No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGTGAC21460.12268507666959753No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC21270.12159886210448927No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTCACCATTATGTCTTCGG21140.12085566266520466No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAACTGGTA20430.11679665034295796No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20190.1154245898396633No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20180.11536742065202603No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19800.11319499152180948No Hit
ACATAATGGTGACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTC19740.11285197639598582No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19650.11233745370725032No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGTGACCAC19630.11222311533197575No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19410.11096539320395565No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT19340.11056520889049472No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19170.10959333270066099No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA19140.10942182513774916No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGCTATAACTAC19130.10936465595011188No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18770.10730656519516987No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG18290.10456244418858057No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGC18150.10376207556165869No Hit
CCCCTGTATTTGTCATCTATGGTAAAACCAACCGGCCCTCAGGGATCCCA18130.10364773718638412No Hit
GTCTGGGGGAGGCCTGGTCAAGCCGGGGGGGTCCCTGAGACTCTCCTGTG18010.10296170693473679No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG17810.10181832318199124No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17540.10027475511578475No Hit
GGGCTGGAGTGGGTCGCATCCATTAGTAGTAGTAGTACTTACATAAATTA17540.10027475511578475No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG17500.10004607836523564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)