FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719999
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCGATCACTTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT54900.7625010590292487No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC47340.6575009131957128No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32700.45416729745457984No Hit
GAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCCTC31540.43805616396689445No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29900.4152783545532702No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTCCAGATACA21740.3019448638123109No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21660.3008337511579877No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19330.26847259510082655No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18730.26013925019340306No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18200.2527781288585123No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17590.2443058948692984No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16400.22777809413624187No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16090.2234725326007397No Hit
CCTTCCACCCTGTCTGCATATGTAGGAGACAGAGTCACCATCACTTGCCG15920.22111141821030306No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15630.21708363483838172No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15180.21083362615781412No Hit
GTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT15120.21000029166707176No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTA14530.20180583584143869No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14230.19763916338772694No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14190.19708360706056538No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13950.19375026909759596No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13880.19277804552506322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13690.19013915297104578No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13430.18652803684449562No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13020.18083358449108958No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12590.17486135397410274No Hit
GTGTATTACTGTGCGAGAGACCCGTACAGCAAACCGATTTATGATATCTGGGGCCAAGGGACAGTGGTCACCGT12030.16708356539384084No Hit
CAGTATAATAGTTATTCGATCACTTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT11780.16361133834908104No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11670.16208355844938674No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11510.15986133314074047No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT11450.15902799864999814No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11450.15902799864999814No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACATA11310.15708355150493267No Hit
GTCTGCATATGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA11190.15541688252344796No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAAGTG11020.15305576813301128No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10880.1511113209879458No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10670.1481946502703476No Hit
GATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA10580.14694464853423406No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC10500.14583353587991094No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10500.14583353587991094No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10070.13986130536292413No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9970.13847241454502018No Hit
CTTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA9920.13777796913606824No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9740.1352779656638412No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCTTCCACC9700.13472240933667964No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9690.13458352025488923No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9550.13263907310982376No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9470.13152796045550064No Hit
GTCTCCTTCCACCCTGTCTGCATATGTAGGAGACAGAGTCACCATCACTT9280.1288890679014832No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA9270.1287501788196928No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9260.12861128973790242No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9250.128472400656112No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9110.12652795351104654No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCGATCACTTTCGGCCAAGGGACACGACTGGAGATTAAACGAA9070.12597239718388498No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCGT8730.12125016840301167No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8620.11972238850331736No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGAC8550.11875016493078464No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8490.11791683044004228No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8420.11694460686750953No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8420.11694460686750953No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8330.11569460513139601No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8310.11541682696781523No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8290.11513904880423445No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGC8280.11500015972244405No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8200.11388904706812093No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8090.11236126716842663No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7960.11055570910515153No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT7930.11013904185978036No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7810.10847237287829566No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7750.10763903838755332No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTCCAGATA7670.1065279257332302No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7670.1065279257332302No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7540.10472236766995509No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7500.10416681134279354No Hit
GTCGTGTCCCTTGGCCGAAAGTGATCGAATAACTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCATCAGG7490.10402792226100314No Hit
TCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC7480.10388903317921275No Hit
CACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTATTACTGCCAACAGTATAATAGTTATTCGATCACTTT7460.10361125501563197No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7430.10319458777026079No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7400.10277792052488961No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7350.10208347511593767No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7340.10194458603414727No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACAACGCCAAGAACTCACTGTATCTGGAAATGAA7310.1015279187887761No Hit
CCCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCG7250.10069458429803374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC150.002482065268.092921
CCGTCAT300.002999157464.9124870
CGTATGC1000.051.80779341
CGGGTAT352.4640163E-548.6141476
TCGTATG1000.048.30285640
GGCGAAT1450.044.79315632
GCGAATC1300.044.72432733
TAAGGCG1450.044.6684829
GTATGCC1350.043.55745342
CTTATAC1500.043.1255151
TTATACA1500.043.1045462
GAGACTA1550.041.74307324
TATACAC1750.040.8358883
CGAATCT1350.040.57412334
ATGCCGT1200.040.46039644
AGACTAA1600.040.4498525
ACGAGAC1600.040.4273622
AGGCGAA1700.038.1659831
GACTAAG1700.038.0757426
ACACATC2000.037.432896
Produced by FastQC (version 0.11.5)