FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719999
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCGATCACTTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT53260.7397232496156245No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC45160.6272230933654075No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32210.44736173244685057No Hit
GAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCCTC28930.4018061136196022No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25830.35875049826458094No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22600.3138893248462845No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTCCAGATACA18610.25847258121191835No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18580.2580559139665472No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17760.24666700925973511No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17630.24486145119646No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16610.23069476485384008No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16490.22902809587235537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14830.20597250829515043No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14640.20333361574113298No Hit
CCTTCCACCCTGTCTGCATATGTAGGAGACAGAGTCACCATCACTTGCCG14180.196944717978775No Hit
GTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT14170.19680582889698459No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13960.19388915817938637No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13900.19305582368864402No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTA13860.19250026736148243No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13810.1918058219525305No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13800.1916669328707401No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13780.1913891547071593No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12780.17750024652812016No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12770.1773613574463298No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12490.17347246315619883No Hit
GTGTATTACTGTGCGAGAGACCCGTACAGCAAACCGATTTATGATATCTGGGGCCAAGGGACAGTGGTCACCGT12110.16819467804816396No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12080.16777801080279278No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11830.164305783758033No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11570.16069466763148282No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11460.1591668877317885No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11350.1576391078320942No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11140.154722437114496No Hit
CAGTATAATAGTTATTCGATCACTTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT11120.15444465895091522No Hit
GTCTGCATATGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10950.15208354456047857No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAAGTG10730.14902798476108994No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGACCCCA10680.148333539352138No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACATA10630.14763909394318603No Hit
GATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA10030.13930574903576254No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC9970.13847241454502018No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9890.13736130189069706No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT9870.1370835237271163No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9740.1352779656638412No Hit
CTTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA9630.1337501857641469No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCTTCCACC9520.1322224058644526No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9370.13013906963759672No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9160.1272223989199985No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9130.12680573167462733No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9030.12541684085672342No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9020.12527795177493303No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8950.12430572820240028No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGC8750.12152794656659245No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8740.12138905748480205No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8720.12111127932122127No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8640.12000016666689815No Hit
GTCTCCTTCCACCCTGTCTGCATATGTAGGAGACAGAGTCACCATCACTT8610.11958349942152698No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8600.11944461033973658No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGAC8590.11930572125794618No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8490.11791683044004228No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA8270.11486127064065366No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCGATCACTTTCGGCCAAGGGACACGACTGGAGATTAAACGAA8240.11444460339528248No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8210.11402793614991132No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7860.10916681828724761No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7860.10916681828724761No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7850.10902792920545723No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCGT7850.10902792920545723No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7830.10875015104187644No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7670.1065279257332302No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7560.10500014583353588No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7500.10416681134279354No Hit
GTCGTGTCCCTTGGCCGAAAGTGATCGAATAACTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCATCAGG7480.10388903317921275No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACAACGCCAAGAACTCACTGTATCTGGAAATGAA7470.10375014409742236No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7460.10361125501563197No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7420.10305569868847039No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7390.10263903144309922No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7380.10250014236130883No Hit
GTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG7320.10166680787056649No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7270.10097236246161453No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7240.10055569521624337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTACG200.00534205756.11231267
CACTAGG200.0077983850.994531
GAACGAA200.00780481250.98389420
ATATCCT200.0078241350.9520239
GTAAGTA200.0078262850.94848612
CCGTCAT555.0291745E-448.16027570
ATTTCCG306.636625E-445.5310431
CCTAACG306.800338E-445.306422
GTATTGA405.4533808E-542.495441
GTGCTAG952.564775E-1035.785631
TCCGAGA4350.035.01307370
GCTCGGG1401.3394074E-633.1101970
GTATCAA14000.032.05371
ATAGGTG3850.031.7600924
CTGGCAA4350.031.45609362
GTGGTAT1950.031.3812481
CGGTACC450.00447037530.87577245
GTAATAC5850.030.8001121
ACACGAC14700.030.77119435
AGGTTGT5900.030.60291929
Produced by FastQC (version 0.11.5)