FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232129
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAATATGCTACCTCACCCCCCCGGTGGACGTTCGGCC42800.34736622545204277No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36850.2990758272875649No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33510.2719682760490176No Hit
CAATAATACACTGCATAATCTTCAGGTTCCACTCTGGTGATGGTGAGAGTGAAGTCTGTCCCTGACCCACTGCCA33310.27034506938802677No Hit
GAAATACACAGCCGTGTCTGCGCCGGTCACAGAATTCAACTGCAGGGAGAAACGATTCCTGGACGTGTCTACGGA31940.2592261037602394No Hit
CTATTATACTGGCAGCACCAAATACAACCCGTCCCTCGAGAGTCGAGTCA31890.2588203020949917No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28840.23406640051488117No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26670.21645460824313037No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26060.21150382792710828No Hit
GTGTATTATTGTCAGCAATATGCTACCTCACCCCCCCGGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAA25460.20663420794413573No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24290.1971384489773392No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22360.1814745046987775No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21430.17392659372517No Hit
CTCCTGCAGGGCCAGTGAGAGTGTTGACAGCAGGTTCTTGACCTGGTACC21210.17214106639808008No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21190.171978745731981No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21150.17165410439978282No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20270.16451199509142306No Hit
GTCCAAGAGGGCCACTGGCGTCCCAGACAGATTCAGTGGCAGTGGGTCAGGGACAGACTTCACTCTCACCATCAC20160.16361923142787807No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20150.16353807109482854No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20060.16280762809738267No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19890.16142790243554042No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19490.15818148911355873No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19460.1579380081144101No Hit
ATAATACACTGCATAATCTTCAGGTTCCACTCTGGTGATGGTGAGAGTGAAGTCTGTCCCTGACCCACTGCCA19350.15704524445086512No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19330.15688292378476604No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18690.1516886624695953No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18320.14868573014676223No Hit
GTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCCGGGGACTGACG18240.14803644748236588No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18220.1478741268162668No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18010.1461697598222264No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17790.14438423249513646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17780.14430307216208693No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17750.1440595911629383No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17740.14397843082988873No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17740.14397843082988873No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17150.13918997117996573No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16910.13724212318677673No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16430.13334642720039866No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16330.13253482386990323No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16290.13221018253770506No Hit
GTGTATTTCTGTGCGAGGGATCGGGGAGTTCTTGTTAGTCGGGACTACTA15990.12977537254621876No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15890.12896376921572336No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15490.12571735589374164No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGT15460.12547387489459302No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC15360.1246622715640976No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15350.12458111123104805No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15270.1239318285666517No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC15270.1239318285666517No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15120.12271442357090856No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14680.11914336891672868No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14450.1172766812565892No Hit
GTCTCTGGTGGCCCCGTCGGAACTACAACTTACAACTGGGCCTGGATCCG14420.11703320025744059No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13980.1134621456032607No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13920.11297518360496345No Hit
GTCTCCGGGCACCTTGTCTTTGTCTCCCGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTGACAG13690.11110849594482397No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13650.11078385461262578No Hit
TTTTTGGAGCGTCCAAGAGGGCCACTGGCGTCCCAGACAGATTCAGTGGCAGTGGGTCAGGGACAGACTTCACT13440.10907948761858538No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13390.10867368595333768No Hit
CTCTTGGACGCTCCAAAAATGAGGACTTTGGGAGCCTGGCCACTTTTCTGCTGGTACCAGGTCAAGAACCTG13030.10575191396355414No Hit
GACTTCACTCTCACCATCACCAGAGTGGAACCTGAAGATTATGCAGTGTATTATTGTCAGCAATATGCTACCTCA12930.10494031063305871No Hit
GCATAATCTTCAGGTTCCACTCTGGTGATGGTGAGAGTGAAGTCTGTCCCTGACCCACTGCCACTGAATCTGT12930.10494031063305871No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT12810.10396638663646421No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG12760.1035605849712165No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG12760.1035605849712165No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA12740.10339826430511741No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATGGTGTTGACGCAGTCTC12610.10234317997547336No Hit
CCTTGGTCCCTTGGCCGAACGTCCACCGGGGGGGTGAGGTAGCATATTGCTGACAATAATACACTGCATAATCTT12580.10209969897632472No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC12560.10193737831022563No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG12560.10193737831022563No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12430.10088229398058157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.044.560891
CGAGACG2150.044.520823
CGTATGC2100.044.46573641
ATCTCGT2000.043.0396237
TCGTATG2350.041.15967640
ATCTCCG2350.040.71526310
GCCGTCT2350.039.971846
GACAGGT2850.039.5704881
CATCTCG2200.039.0853736
CACGAGA2450.039.0629321
GTATCAA25100.038.5314251
ACGAGAC2500.038.28322622
TATACAC2450.037.6555943
TACGAAA700.001707908137.5710770
CCCACGA2550.037.5203919
CGTCTTC2550.036.9145648
GGAGCTA2650.036.1735329
ACGGAGC2650.036.15147827
AGACGGA2650.036.13385425
CGAGCCC2650.036.10305815
Produced by FastQC (version 0.11.5)