FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232129
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAATATGCTACCTCACCCCCCCGGTGGACGTTCGGCC41110.33365012916667003No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37230.3021599199434475No Hit
CTATTATACTGGCAGCACCAAATACAACCCGTCCCTCGAGAGTCGAGTCA29330.23804325683430874No Hit
CAATAATACACTGCATAATCTTCAGGTTCCACTCTGGTGATGGTGAGAGTGAAGTCTGTCCCTGACCCACTGCCA29200.23698817250466467No Hit
GAAATACACAGCCGTGTCTGCGCCGGTCACAGAATTCAACTGCAGGGAGAAACGATTCCTGGACGTGTCTACGGA29130.23642005017331788No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28180.22870981853361133No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26180.2124777519237028No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26060.21150382792710828No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25910.21028642293136512No Hit
GTGTATTATTGTCAGCAATATGCTACCTCACCCCCCCGGTGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAA25720.20874437660342382No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22850.18545136101820509No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22350.18139334436572793No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21840.17725416738020125No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21370.17343963172687277No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20330.16499895708972032No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19960.16199602476688724No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19610.15915541311015324No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19310.15672060311866695No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19180.1556655187890229No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19170.15558435845597338No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18910.15347418979668523No Hit
CTCCTGCAGGGCCAGTGAGAGTGTTGACAGCAGGTTCTTGACCTGGTACC18860.15306838813143753No Hit
ATAATACACTGCATAATCTTCAGGTTCCACTCTGGTGATGGTGAGAGTGAAGTCTGTCCCTGACCCACTGCCA18760.1522567848009421No Hit
GTCCAAGAGGGCCACTGGCGTCCCAGACAGATTCAGTGGCAGTGGGTCAGGGACAGACTTCACTCTCACCATCAC17780.14430307216208693No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17750.1440595911629383No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17510.14211174316974928No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17270.14016389517656025No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17210.139676933178263No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16750.13594355785798404No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16700.1355377561927363No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16670.1352942751935877No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16610.13480731319529043No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16520.13407687019784453No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16290.13221018253770506No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16210.1315608998733087No Hit
GTGTATTTCTGTGCGAGGGATCGGGGAGTTCTTGTTAGTCGGGACTACTA16070.1304246552106151No Hit
GTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCCGGGGACTGACG15930.12928841054792153No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15710.1275028832208316No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15680.12725940222168297No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15670.12717824188863341No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15550.1262043178920389No Hit
GTCTCTGGTGGCCCCGTCGGAACTACAACTTACAACTGGGCCTGGATCCG15360.1246622715640976No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14830.12036077391247182No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14750.11971149124807548No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14500.11768248292183692No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14460.11735784158963875No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14410.11695203992439103No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGT14350.11646507792609379No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14310.1161404365938956No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14160.11492303159815245No Hit
TTTTTGGAGCGTCCAAGAGGGCCACTGGCGTCCCAGACAGATTCAGTGGCAGTGGGTCAGGGACAGACTTCACT13970.11338098527021115No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13620.11054037361347717No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13620.11054037361347717No Hit
GGGCTGGTGAAGGCTTCGGAGACCCTGTCCCTCACCTGCATTGTCTCTGG13610.11045921328042763No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13500.10956644961688264No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13450.10916064795163494No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC13430.10899832728553584No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13410.10883600661943676No Hit
GTCTCCGGGCACCTTGTCTTTGTCTCCCGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTGACAG13120.10648235696100003No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12840.10420986763561282No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12630.10250550064157243No Hit
CTCTTGGACGCTCCAAAAATGAGGACTTTGGGAGCCTGGCCACTTTTCTGCTGGTACCAGGTCAAGAACCTG12590.10218085930937426No Hit
GGGCTGGAGTGGATTGGGAGTATCTATTATACTGGCAGCACCAAATACAA12540.10177505764412656No Hit
GTCCTGTCCCAGCTGCAGCTCCAAGAGTCGGGCCCAGGGCTGGTGAAGGC12520.10161273697802747No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC12420.10080113364753203No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12350.10023301131618523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.045.8352131
ACCGTAT350.001166516440.6043260
CGCGACC400.002740769234.12135712
GTATCAA26900.033.900691
CAAACGA20350.031.58546370
GACAGGT1900.030.5594061
ATCAACG30400.029.5194633
TATCAAC31200.029.2094922
TCAACGC30900.029.0418054
AACGCAG31750.028.8016516
AATGGTA3500.028.69482870
CAACGCA31700.028.5241625
ACGCAGA33550.027.4598127
TCGCATC500.00812109427.32592223
CGCAGAG33650.027.2768068
AGAGTAC34400.026.38453911
CGCATCA659.1768696E-426.27492524
TAATGTC659.23361E-426.2471965
GAGTTTA6250.026.2307531
GCAGAGT34800.025.9832179
Produced by FastQC (version 0.11.5)