FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219166
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36180.29676024429815134No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33030.27092290959557597No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTGCTCTTCCTCCTGCTACTCTGGCTCCCAGAATACA27800.22802473166082388No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGACCCTGAGGATTCTGCAGTTTATTACTGTCAGCAGT25970.21301447054789913No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24940.20456607221658085No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21740.1783186210901551No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21660.17766243481199442No Hit
GCACAGAACTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGTCTCCACCAGGGCCACTGGCATCCCAGACAGGTT21650.17758041152722434No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20750.1701983158979171No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20220.16585108180510283No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20040.16437466267924136No Hit
CTCCTGCAGGGCCAGTCAGATTGTTGGCATGGAAGACCTAGCCTGGTACC19530.1601914751559673No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT19440.15945326559303655No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19430.15937124230826646No Hit
GAACTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGTCTCCACCAGGGCC19300.15830493960625544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19270.1580588697519452No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18980.15568019449361284No Hit
GTTTATTACTGTCAGCAGTCTCGCAGTTTACCGTTCACTTTCGGCGGGGGGACCAAGGTGGAGGTCAAACGAA18900.1550240082154522No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18540.1520711699637293No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18380.15075879740740802No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18180.1491183317120064No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18110.14854416871861584No Hit
CATTTATGGTGTCTCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA17950.14723179616229454No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17450.14313063192379052No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17060.13993172381775737No Hit
GTTCTGTGCTGGTACCAGGCTAGGTCTTCCATGCCAACAATCTGACTGGCCCTGCAGGAGAGGGTGGCTCGTTCC16730.13722495542034474No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCT16670.13673281571172424No Hit
GTGTTCCCTGGCCCCAGTAGGCAAACTTCCGAATCCAGTTGTCACGGTAACCGGGGTCACTCGCACAAAAATAGA16660.13665079242695416No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16510.13542044315540297No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16490.13525639658586278No Hit
CCCCTGGACAAGGCCTTGAGTGGATGGGTTACATCAACCCTAACAGCGGT16400.13451818702293206No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16090.13197546519505957No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGC15760.1292686967976469No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15230.12492146270483265No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15040.12336302029420111No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15000.1230349271551208No Hit
GTAATAAACTGCAGAATCCTCAGGGTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA14760.12106636832063887No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14500.11893376291661677No Hit
GTCTGGGGCTGAAGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCAAGACCTCGGGATACAGTTTCAGTTC14310.11737532050598523No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14300.11729329722121515No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14280.117129250651675No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14260.11696520408213484No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCCCCGCCGAAAGTGAACGGTAAA14120.11581687809535371No Hit
GACTTCACTCTCACCATCAGCAGCCTGGACCCTGAGGATTCTGCAGTTTATTACTGTCAGCAGTCTCGCAGTTTA13960.11450450553903242No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCCCCGCCGAAAGTG13840.11352022612179145No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13660.11204380699593001No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGTTCTGTGCTGGTACCAGGCTAGGTCTTCCATGCCAACAATCTGA13540.11105952757868903No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13520.11089548100914888No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTGCTCTTCCTCCTGCTACTCTGGCTCCCAGAATA13270.10884489888989687No Hit
GTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13170.10802466604219606No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13140.10777859618788582No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13130.10769657290311573No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13020.10679431677064485No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12890.1057280140686338No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12750.10457968808185267No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12650.10375945523415186No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12530.1027751758169109No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12500.10252910596260066No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTGCTCT12450.10211898953875027No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12220.1002324539890384No Hit
GGATGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGTTACATCAACCCTAACAGCGGTGCCATAAGTTATG12200.10006840741949825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2050.045.51440441
ATGCCGT2100.044.62604544
TCGTATG2100.044.38262640
GTCGTAT503.8685284E-641.3600639
GGTATCA13500.039.5523831
TAACGGC1150.038.77216736
ATCTCCG2600.037.9264610
CGGTACC1200.037.64728545
CGCGGTA1200.037.5434243
GAGCGAT2500.036.9216632
CTTATAC2800.036.4392931
TATACAC2800.036.4363063
TGAGCGA2550.036.16787331
ATCTCGT1900.036.1544937
GTATCAA33750.036.0758061
ACGAGAC2750.035.86657322
TTAACGG1250.035.64532535
CGAGCCC2700.035.26096315
GTGGTAT4150.034.4197731
CGAATAG400.002737186834.13042430
Produced by FastQC (version 0.11.5)