FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219166
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35800.2936433594768883No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27050.22187298530306782No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGACCCTGAGGATTCTGCAGTTTATTACTGTCAGCAGT26090.2139987499651401No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTGCTCTTCCTCCTGCTACTCTGGCTCCCAGAATACA25860.21211221441542827No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25370.2080930734616943No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24820.20358179279933988No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22750.1866029728519332No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20310.16658929136803355No Hit
GTGTTCCCTGGCCCCAGTAGGCAAACTTCCGAATCCAGTTGTCACGGTAACCGGGGTCACTCGCACAAAAATAGA19870.16298026683815No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19260.1579768464671751No Hit
GCACAGAACTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGTCTCCACCAGGGCCACTGGCATCCCAGACAGGTT19220.15764875332809478No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18760.15387568222867107No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18410.15100486726171827No Hit
GAACTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGTCTCCACCAGGGCC18320.15026665769878753No Hit
CTCCTGCAGGGCCAGTCAGATTGTTGGCATGGAAGACCTAGCCTGGTACC18240.1496104714206269No Hit
GTTTATTACTGTCAGCAGTCTCGCAGTTTACCGTTCACTTTCGGCGGGGGGACCAAGGTGGAGGTCAAACGAA18150.14887226185769617No Hit
CATTTATGGTGTCTCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA18100.14846214543384578No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17960.14731381944706462No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17770.1457553770364331No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT17630.14460705104965196No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17600.14436098119534171No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16940.13894744440051643No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16930.13886542111574632No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16690.1368968622812644No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16320.13386200074477142No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16310.13377997746000134No Hit
GTTCTGTGCTGGTACCAGGCTAGGTCTTCCATGCCAACAATCTGACTGGCCCTGCAGGAGAGGGTGGCTCGTTCC16250.13328783775138087No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCT15800.12959678993672724No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15640.12828441738040594No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15300.1254956256982232No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15260.1251675325591429No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14950.12262481073127039No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14690.1204922053272483No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14580.11958994919477742No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14310.11737532050598523No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14160.11614497123443404No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14140.11598092466489386No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14120.11581687809535371No Hit
GACTTCACTCTCACCATCAGCAGCCTGGACCCTGAGGATTCTGCAGTTTATTACTGTCAGCAGTCTCGCAGTTTA14030.11507866853242299No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGC14000.11483259867811274No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13940.11434045896949227No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13880.11384831926087179No Hit
GTCTGGGGCTGAAGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCAAGACCTCGGGATACAGTTTCAGTTC13880.11384831926087179No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCCCCGCCGAAAGTG13780.11302808641317097No Hit
CCCCTGGACAAGGCCTTGAGTGGATGGGTTACATCAACCCTAACAGCGGT13620.11171571385684968No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13560.11122357414822921No Hit
GTAATAAACTGCAGAATCCTCAGGGTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA13540.11105952757868903No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13400.10991120159190791No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13220.10843478246604646No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13150.1078606194726559No Hit
GTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13120.10761454961834567No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGTTCTGTGCTGGTACCAGGCTAGGTCTTCCATGCCAACAATCTGA13040.106958363340185No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG12820.10515385107524323No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCCCCGCCGAAAGTGAACGGTAAA12790.104907781220933No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCAGTGTTCCCTGG12770.10474373465139285No Hit
GTCCAGGGGCCTGTCGCATCCAGTGTAGATAGTAGGAACTGAAACTGTATCCCGAGGTCTTGCAGGAGACCCTCA12740.10449766479708261No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12730.10441564151231252No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12610.10343136209507156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.041.1553271
GTATCAA35150.034.683351
GTGGTAT3950.034.3886261
CTAACGC400.002814737633.9364783
TACGTAC1450.032.76356512
CCTAACG450.00500050930.1781372
ACGACGG450.005011557630.16451611
TATCAAC40550.030.0572012
GTAGTTT2251.6789272E-930.03638370
ATACGTA1600.029.69320111
ATCAACG40800.029.611243
TCAACGC41250.029.2870084
CAACGCA41450.029.0638265
ATGGCAA3500.028.96365470
AACGCAG41700.028.889586
TGGTATC4600.028.7840372
CGCAGAG43300.027.9788118
GAATACG1700.027.9465399
AATACGT1700.027.94653910
AGAGTAC44100.027.39430811
Produced by FastQC (version 0.11.5)