FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225009
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68820.5617917909174545No Hit
GACTTACAGTGCCCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT65400.5338736286835444No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61520.5022003919971201No Hit
CTGTAAGTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACT52130.42554789393384046No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48430.39534403420709563No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45950.375099284984845No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG45070.36791566429307865No Hit
ACCTACTACTGTCAACAGACTTACAGTGCCCCTCCGCTCACTTTCGGCGG41000.3346914185936593No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCACCCATTTAAATTGGTATCAGCAGAAG39990.3264465812087911No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38740.3162425745443503No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37410.3053855114533852No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCGCAGATCCACTGCCA37200.3036712383337592No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAC36270.2960794573754152No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36230.2957529291621531No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35560.29028358159001283No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG33880.27656939663300434No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33860.27640613252637325No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33540.27379390682027643No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33030.2696306721011846No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32710.2670184463950877No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32460.26497764506219956No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGACTTACAGTGCCCCTCCGCTCA32390.26440622068899083No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31490.25705933589059343No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC30640.2501206113587737No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC30000.24489615994657998No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28340.23134523909620255No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT28340.23134523909620255No Hit
CTACTACTGTCAACAGACTTACAGTGCCCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA28290.23093707882962494No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT26990.22032491189860645No Hit
AATCTATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGCGACAGATTTCA25900.21142701808721406No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25830.21085559371400536No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25050.2044882935553943No Hit
GTGCCCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA24920.20342707686229244No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24890.20318218070234587No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24750.2020393319559285No Hit
GAGGGGCACTGTAAGTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGT23320.19036594833180817No Hit
CAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCGCAGATCCACTGCCA23220.18954962779865292No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT23200.18938636369202186No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22850.1865292418259784No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC22700.18530476102624552No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22660.18497823281298342No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21360.17436606588196493No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA21000.171427311962606No Hit
CCATAGATTAGGAGGTTTGGGGCTCTCCCTGGCTTCTGCTGATACCAATT20950.17101915169602835No Hit
GCCAGGGAGAGCCCCAAACCTCCTAATCTATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCAAGGTTCAGTGG20780.1696314067896644No Hit
GCCCCAAACCTCCTAATCTATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT20660.1686518221498781No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20570.16791713367003835No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20540.16767223751009175No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20040.16359063484431544No Hit
CTCCCTGGCTTCTGCTGATACCAATTTAAATGGGTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACT20030.1635090027909999No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19900.16244778609789806No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19890.16236615404458252No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19250.15714170263238883No Hit
AGTCAGAGCATTAGCACCCATTTAAATTGGTATCAGCAGAAGCCAGGGAG18710.1527335717533504No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18350.14979481783399143No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18160.14824380882099641No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17940.14644790364805482No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17830.14554995106158405No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17590.1435907817820114No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG17560.1433458856220648No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17440.14236630098227848No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17370.1417948766090698No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17370.1417948766090698No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT17300.14122345223586114No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17270.14097855607591456No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGCGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTG17260.140896924022599No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16850.13755000983666243No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16740.13665205725019164No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16650.13591736877035188No Hit
GTTCAGTGGCAGTGGATCTGCGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACCTA16570.13526431234382766No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC16540.13501941618388108No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16420.13403983154409477No Hit
GTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGACTTACAGT16280.13289698279767742No Hit
GGTGATGGTGAGAGTGAAATCTGTCGCAGATCCACTGCCACTGAACCTTG16220.13240719047778426No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16000.13061128530484264No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15940.1301214929849495No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15860.1294684365584253No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15830.12922354039847872No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGCGACAGATTTCACT15830.12922354039847872No Hit
CTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGCGACAGATTTCACTCTCACCATCACCAGTCTG15740.12848885191863896No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT15600.12734600317222158No Hit
GATTAGGAGGTTTGGGGCTCTCCCTGGCTTCTGCTGATACCAATTTAAAT15590.12726437111890607No Hit
GAGTGAAATCTGTCGCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15380.12555009799928002No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15220.12424398514623157No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGGGCACTGTAAGTCTGTTGA15110.12334603255976079No Hit
CCTCCTAATCTATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCAAGGT15090.12318276845312973No Hit
GCAGAAGCCAGGGAGAGCCCCAAACCTCCTAATCTATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCAAGGTT15050.12285624023986763No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14950.12203991970671235No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA14910.12171339149345026No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14870.12138686328018815No Hit
CATTTAAATTGGTATCAGCAGAAGCCAGGGAGAGCCCCAAACCTCCTAAT14830.12106033506692604No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14810.12089707096029499No Hit
ACCTTGATGGGACCCCACTTTGCAGTCTGGATGCACCATAGATTAGGAGGTTTGGGGCTCTCCCTGGCTTCTG14750.12040727864040184No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14740.12032564658708629No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14740.12032564658708629No Hit
GTGATGGTGAGAGTGAAATCTGTCGCAGATCCACTGCCACTGAACCTTGA14410.11763178882767392No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14270.11648894008125656No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT14000.11428487464173734No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13860.11314202589531995No Hit
GCATTAGCACCCATTTAAATTGGTATCAGCAGAAGCCAGGGAGAGCCCCA13810.11273386562874231No Hit
GAGCATTAGCACCCATTTAAATTGGTATCAGCAGAAGCCAGGGAGAGCCCCAAACCTCCTAATCTATGGTGCATC13810.11273386562874231No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13640.11134612072237837No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13620.11118285661574731No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGGGCACTGTAAGTCTGTTG13510.11028490402927651No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13480.11004000786932994No Hit
GCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGCGACAGATT13370.10914205528285915No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13270.10832573474970388No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13080.10677472573670888No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12990.10604003725686914No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG12970.10587677315023808No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGACTTACAGTGCC12890.10522371672371388No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT12840.10481555645713624No Hit
CACCATCACTTGCCGGGCAAGTCAGAGCATTAGCACCCATTTAAATTGGT12770.10424413208392756No Hit
GGTTTGGGGCTCTCCCTGGCTTCTGCTGATACCAATTTAAATGGGTGCTA12440.10155027432451516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTCG200.007802753250.9894429
TCGTATG4850.041.24052440
ATGCCGT5100.040.1552544
CGTATGC5100.039.961541
GTACGCC350.001414931739.04086734
GTATCAA25500.038.297991
ACGAGAC5650.037.807722
GTATGCC5600.036.46327642
ATCTCGT5100.036.32111737
GCCCACG5900.036.19681518
CCCACGA5900.036.19681519
TATACAC6350.035.7670333
CACGAGA5900.035.62808221
CCGTCTT5400.035.53445447
TCTCCGA5950.035.32147611
CTTATAC6150.035.288121
CGTCTTC5450.034.64252548
TACACAT6250.034.1697925
AGCCCAC6250.034.168417
GATCTCG5550.033.93676436
Produced by FastQC (version 0.11.5)