FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225009
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69140.5644040166235513No Hit
GACTTACAGTGCCCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT62430.5096289088488329No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53170.43403762747865526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51770.4226091400144815No Hit
CTGTAAGTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACT49250.4020378625789688No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49170.40138480615244454No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG45760.37354827597185No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40800.33305877752734875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39090.3190996964103937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39030.31860990409050055No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCACCCATTTAAATTGGTATCAGCAGAAG38700.31591604633108816No Hit
ACCTACTACTGTCAACAGACTTACAGTGCCCCTCCGCTCACTTTCGGCGG38070.31077322697221No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCGCAGATCCACTGCCA36170.29526313684225997No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35240.28767135588391596No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAC33870.2764877645796888No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33600.2742836991401696No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33470.2732224824470677No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32530.26554906943540824No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG31320.2556715909842295No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30060.24538595226647314No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC29950.24448799968000237No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29610.24171250986727444No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGACTTACAGTGCCCCTCCGCTCA29430.24024313290759497No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29400.2399982367476484No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29240.23869212389459996No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29050.23714111488160497No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28150.22979423008320757No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC27510.22456977867101385No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27060.22089633627181515No Hit
CTACTACTGTCAACAGACTTACAGTGCCCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA26130.21330455531347117No Hit
AATCTATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGCGACAGATTTCA26070.21281476299357802No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26030.2124882347803159No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG24680.2014679075827198No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT23900.19510060742410873No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23830.19452918305090003No Hit
GAGGGGCACTGTAAGTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGT23450.19142716502491003No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23230.18963125985196844No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA22140.18073336604057602No Hit
GTGCCCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA21970.17934562113421207No Hit
CAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCGCAGATCCACTGCCA21940.1791007249742655No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC21350.17428443382864942No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGCGACAGATTTCACT20920.17077425553608178No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20600.16816202982998493No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19880.162284521991267No Hit
CTCCCTGGCTTCTGCTGATACCAATTTAAATGGGTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACT19850.16203962583132042No Hit
GCCAGGGAGAGCCCCAAACCTCCTAATCTATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCAAGGTTCAGTGG19720.1609784091382186No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19650.1604069847650099No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19630.16024372065837886No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19600.15999882449843225No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19570.15975392833848567No Hit
CCATAGATTAGGAGGTTTGGGGCTCTCCCTGGCTTCTGCTGATACCAATT19480.15901923985864594No Hit
GCCCCAAACCTCCTAATCTATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT19470.1589376078053304No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19330.15779475905891305No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18850.15387642049976777No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18560.15150909095361748No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18450.15061113836714668No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18280.14922339346078276No Hit
AGTCAGAGCATTAGCACCCATTTAAATTGGTATCAGCAGAAGCCAGGGAG18180.14840707292762748No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18090.1476723844477877No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17990.14685606391463246No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17740.1448152625817443No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17520.1430193574088027No Hit
GTTCAGTGGCAGTGGATCTGCGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACCTA17080.13942754706291952No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17070.139345915009604No Hit
GGTGATGGTGAGAGTGAAATCTGTCGCAGATCCACTGCCACTGAACCTTG16530.13493778413056556No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16460.1343663597573569No Hit
GTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGACTTACAGT16440.13420309565072583No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16360.1335500392242016No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG16240.1325704545844153No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16170.1319990302112066No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16160.13191739815789108No Hit
GAGTGAAATCTGTCGCAGATCCACTGCCACTGAACCTTGATGGGACCCCA16070.13118270967805135No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC16050.13101944557142028No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15840.12930517245179424No Hit
CTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGCGACAGATTTCACTCTCACCATCACCAGTCTG15680.1279990595987458No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15660.12783579549211474No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGCGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTG15640.12767253138548368No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15610.1274276352255371No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15530.1267745787990129No Hit
CCTCCTAATCTATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCAAGGT15230.12432561719954711No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA15030.12269297613323657No Hit
GATTAGGAGGTTTGGGGCTCTCCCTGGCTTCTGCTGATACCAATTTAAAT14970.12220318381334341No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14900.12163175944013474No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14840.12114196712024157No Hit
CATTTAAATTGGTATCAGCAGAAGCCAGGGAGAGCCCCAAACCTCCTAAT14500.11836647730751365No Hit
ACCTTGATGGGACCCCACTTTGCAGTCTGGATGCACCATAGATTAGGAGGTTTGGGGCTCTCCCTGGCTTCTG14480.11820321320088262No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14400.1175501567743584No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14380.11738689266772734No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14330.11697873240114971No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGACTTACAGTGCC14180.11575425160141681No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14060.1147746669616305No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT14020.11444813874836837No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14020.11444813874836837No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13990.1142032425884218No Hit
GCAGAAGCCAGGGAGAGCCCCAAACCTCCTAATCTATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCAAGGTT13980.11412161053510628No Hit
GTGATGGTGAGAGTGAAATCTGTCGCAGATCCACTGCCACTGAACCTTGA13900.11346855410858206No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGGGCACTGTAAGTCTGTTGA13420.10955021554943678No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13370.10914205528285915No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13280.10840736680301942No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13280.10840736680301942No Hit
GAGCATTAGCACCCATTTAAATTGGTATCAGCAGAAGCCAGGGAGAGCCCCAAACCTCCTAATCTATGGTGCATC13190.10767267832317967No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12930.10555024493697597No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12900.10530534877702939No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12840.10481555645713624No Hit
GCATTAGCACCCATTTAAATTGGTATCAGCAGAAGCCAGGGAGAGCCCCA12540.10236659485767044No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12420.10138701021788411No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG12380.10106048200462199No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12360.10089721789799096No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12360.10089721789799096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA150.002535215367.73286416
GTGGTAT2400.042.3572581
GTATCAA27200.039.2427561
CTAACGC451.1197773E-437.614013
TATCAAC30350.034.371382
TCGCACG400.002832028533.89411522
GTTTCGG400.002833731233.8899621
ATCAACG31500.033.100333
TACGTAT1002.3958705E-433.04588770
TCAACGC31800.032.678944
CAACGCA32050.032.422715
AACGCAG32700.032.0887576
CCGGTTT450.003817928531.88900259
GATTCAC3050.031.1082841
AGAGTAC34650.030.37934111
CGCAGAG35300.030.0117198
ACGCAGA35000.029.9800687
GCAGAGT35850.029.64579
TGTTAGA17650.029.58215570
ACACCGT606.062209E-428.2070526
Produced by FastQC (version 0.11.5)