FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919738
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41320.4492583757548345No Hit
GAGTTTACACTGAAAATTAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGTTCTGGAAACT39820.4329493834113628No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39430.4287090454020602No Hit
CCCCTGGAGAGCCGGCCTCAATCTCCTGCAGGTCTAGTCAGAGCCTCCTG31250.3397706738223277No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27950.30389089066668984No Hit
GTGTAAACTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG27130.294975308185592No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25390.27605687706716475No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23240.2526806547081886No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23150.25170211516758034No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22770.24757050377390086No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGAAACAACTATTTGGACTGGTATCTGCAGAAGCCAGGGCAGTCTC22100.2402858205271501No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21470.233436043742892No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21310.2316964178929217No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTAATTTTCAGTGTAAACTCTGTGCCTGATCCACTGCCA20720.22528154757115612No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20080.21832304417127488No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19960.2170183247837971No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19890.21625723847443512No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19820.2154961521650731No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19670.2138652529307259No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19150.20821146891832237No Hit
GTTTATTACTGCATGCAAGTTCTGGAAACTCCTGGTTTTGGCCAGGGGAC18930.20581948337461323No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18390.19994824613096338No Hit
GGCACAGAGTTTACACTGAAAATTAGCAGAGTGGAGGCTGAGGATGTTGG17700.1924461096529664No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17340.18853195149053317No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCC17240.18744468533430172No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA16450.17885528270007328No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15790.17167932606894573No Hit
GTCTCCACTCTACCTGCCCGTCACCCCTGGAGAGCCGGCCTCAATCTCCT15480.16830880098462822No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAACCA15480.16830880098462822No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGAGTTTACACTGAA15430.16776516790651252No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCC14950.16254629035660154No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTAC14720.16004557819726922No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTACCTGCCCGTCACCC14560.1583059523472989No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14550.15819722573167577No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14540.1580884991160526No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14530.15797977250042947No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGATA14410.15667505311295174No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14020.15243471510364906No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13940.15156490217866392No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13550.14732456416936127No Hit
GTAATGGAAACAACTATTTGGACTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTT13500.14678093109124554No Hit
CCACTCTACCTGCCCGTCACCCCTGGAGAGCCGGCCTCAATCTCCTGCAG13250.14406276570066692No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13140.14286677292881234No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT12870.13993115430698744No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT12710.1381915284570171No Hit
CTCCAGGGGTGACGGGCAGGTAGAGTGGAGACTGAGTCATCACAATATCC12590.13688680906953937No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12530.13623444937580048No Hit
CTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCC12410.13492972998832276No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12010.13058066536339696No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11980.13025448551652752No Hit
GTTCAGTGGCAGTGGATCAGGCACAGAGTTTACACTGAAAATTAGCAGAG11940.12981957905403496No Hit
CTCCTGGTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC11860.1289497661290498No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11820.1285148596665572No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTAATTTTCAGTGTAAACTCTGTGCCTGATCCACTGCCA11790.1281886798196878No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11530.12536178781348603No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11280.1226436224229074No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11270.12253489580728424No Hit
AGTCAGAGCCTCCTGCATAGTAATGGAAACAACTATTTGGACTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAA11160.12133890303542966No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTTCTGGTGGCAGCTCCCAGATGGGTC11080.1204690901104445No Hit
CTACCTGCCCGTCACCCCTGGAGAGCCGGCCTCAATCTCCTGCAGGTCTA11040.1200341836479519No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11000.11959927718545933No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10910.11862073764485102No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10900.11851201102922788No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10850.11796837795111216No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTACCTGCCCGTCACCCCTGGAGAGCCGGCCTCAATCTCCTGCAGGT10850.11796837795111216No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10620.11546766579177982No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10590.1151414859449104No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10570.11492403271366411No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10490.11405421978867894No Hit
GCCCTGGCTTCTGCAGATACCAGTCCAAATAGTTGTTTCCATTACTATGC10480.1139454931730558No Hit
CTTCAATACTTACTACTGGACCTGGATTCGCCAGCCCCCAGGGAAGGGGC10470.11383676655743265No Hit
TTATTACTGCATGCAAGTTCTGGAAACTCCTGGTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGG10330.11231459393870863No Hit
CTCCTGCATAGTAATGGAAACAACTATTTGGACTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT10310.11209714070746235No Hit
CTATTTGGACTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTC10260.11155350762934661No Hit
GACTAGACCTGCAGGAGATTGAGGCCGGCTCTCCAGGGGTGACGGGCAGG10140.11024878824186887No Hit
GCTTGGTCCCCTGGCCAAAACCAGGAGTTTCCAGAACTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCA10100.1098138817793763No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10080.10959642854813001No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10070.10948770193250687No Hit
GGTATCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGT10030.1090527954700143No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGTTCTGGAA10020.10894406885439115No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10010.108835342238768No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9970.10840043577627542No Hit
AACCAGGAGTTTCCAGAACTTGCATGCAGTAATAAACTCCAACATCCTCA9940.10807425592940598No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9910.10774807608253655No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9650.10492118407633479No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9640.10481245746071163No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGTTCTGGAAACTCCTGGTTTTGGCCAGGGGACCAAG9620.10459500422946534No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9520.10350773807323391No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9510.10339901145761075No Hit
CTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT9400.10220301868575617No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9320.10133320576077101No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9310.10122447914514786No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9260.10068084606703215No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9240.10046339283578584No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9210.1001372129889164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)