FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919738
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40590.441321332814345No Hit
GAGTTTACACTGAAAATTAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGTTCTGGAAACT40480.4401253400424903No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32090.3489037095346718No Hit
GTGTAAACTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG28750.3125890199165414No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28600.3109581206821943No Hit
CCCCTGGAGAGCCGGCCTCAATCTCCTGCAGGTCTAGTCAGAGCCTCCTG27410.29801965342304004No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23530.2558337265612598No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23380.25420282732691263No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23270.25300683455505807No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGAAACAACTATTTGGACTGGTATCTGCAGAAGCCAGGGCAGTCTC22950.24952758285511742No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21910.23822001483031038No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTAATTTTCAGTGTAAACTCTGTGCCTGATCCACTGCCA20690.22495536772428668No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20450.22234592894933122No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20060.2181055909400286No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19440.21136454077139358No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19410.21103836092452416No Hit
GTTTATTACTGCATGCAAGTTCTGGAAACTCCTGGTTTTGGCCAGGGGAC19310.2099510947682927No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18310.19907843320597823No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17890.19451191534980614No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17860.1941857355029367No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17810.19364210242482097No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17220.18722723210305542No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17200.18700977887180914No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16730.18189962793752132No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15840.17222295914706146No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCC15710.17080951314396056No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15550.16906988729399025No Hit
GTCTCCACTCTACCTGCCCGTCACCCCTGGAGAGCCGGCCTCAATCTCCT15390.1673302614440199No Hit
GGCACAGAGTTTACACTGAAAATTAGCAGAGTGGAGGCTGAGGATGTTGG15290.1662429952877885No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15270.16602554205654219No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAACCA14760.1604804846597618No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGAGTTTACACTGAA14610.15884958542541464No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTAC14610.15884958542541464No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCC14370.15624014665045916No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTACCTGCCCGTCACCC14270.1551528804942277No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14240.15482670064735826No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13860.15069508925367878No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA13810.15015145617556303No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGATA13170.14319295277568175No Hit
CTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCC12850.13971370107574113No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT12820.13938752122887169No Hit
GTAATGGAAACAACTATTTGGACTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTT12700.13808280184139396No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12390.13471227675707645No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12260.13329883075397558No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12190.13253774444461358No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT12140.13199411136649786No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11880.1291672193602961No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTAATTTTCAGTGTAAACTCTGTGCCTGATCCACTGCCA11870.12905849274467293No Hit
CTCCAGGGGTGACGGGCAGGTAGAGTGGAGACTGAGTCATCACAATATCC11850.12884103951342665No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11680.12699268704783317No Hit
CTCCTGGTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC11600.12612287412284803No Hit
GTTCAGTGGCAGTGGATCAGGCACAGAGTTTACACTGAAAATTAGCAGAG11590.1260141475072249No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11480.12481815473537028No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11470.12470942811974714No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11450.12449197488850086No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11320.12307852888539998No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11230.12209998934479167No Hit
GGTATCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGT11210.12188253611354538No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTACCTGCCCGTCACCCCTGGAGAGCCGGCCTCAATCTCCTGCAGGT11000.11959927718545933No Hit
CTCCTGCATAGTAATGGAAACAACTATTTGGACTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT10880.1182945577979816No Hit
CCACTCTACCTGCCCGTCACCCCTGGAGAGCCGGCCTCAATCTCCTGCAG10860.11807710456673531No Hit
GCTTGGTCCCCTGGCCAAAACCAGGAGTTTCCAGAACTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCA10860.11807710456673531No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10690.11622875210114184No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10580.11503275932928725No Hit
TTATTACTGCATGCAAGTTCTGGAAACTCCTGGTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGG10470.11383676655743265No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10470.11383676655743265No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10440.11351058671056323No Hit
CTACCTGCCCGTCACCCCTGGAGAGCCGGCCTCAATCTCCTGCAGGTCTA10250.11144478101372349No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTTCTGGTGGCAGCTCCCAGATGGGTC10210.11100987455123089No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGATACCAGTCCAAATAGTTGTTTCCATTACTATGCAGGAGGCTCTGA10160.11046624147311518No Hit
CTATTTGGACTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTC10110.10992260839499945No Hit
CTTCAATACTTACTACTGGACCTGGATTCGCCAGCCCCCAGGGAAGGGGC10090.10970515516375316No Hit
GCCCTGGCTTCTGCAGATACCAGTCCAAATAGTTGTTTCCATTACTATGC10080.10959642854813001No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10080.10959642854813001No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9920.10785680269815968No Hit
AGTCAGAGCCTCCTGCATAGTAATGGAAACAACTATTTGGACTGGTATCTGCAGAAGCCAGGGCAGTCTCCACAA9780.10633463007943567No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9770.10622590346381251No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9760.10611717684818939No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9730.10579099700131994No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9690.10535609053882736No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9680.10524736392320422No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGTTCTGGAAACTCCTGGTTTTGGCCAGGGGACCAAG9650.10492118407633479No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9630.10470373084508848No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9630.10470373084508848No Hit
AACCAGGAGTTTCCAGAACTTGCATGCAGTAATAAACTCCAACATCCTCA9550.10383391792010332No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9540.1037251913044802No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGTTCTGGAA9500.1032902848419876No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9480.10307283161074131No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9440.10263792514824874No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9400.10220301868575617No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9300.10111575252952472No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT9230.1003546662201627No Hit
CTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT9210.1001372129889164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA200.00779896550.9946941
GGTATCA8250.043.2682271
TACGTAT750.002254284735.50371670
GTATCAA19700.035.204461
AATGGTA3250.032.7726670
GATTCAC1155.638867E-1132.5183561
GTGGTAT2450.031.9150471
TATCAAC22650.030.4592952
ATCAACG22900.030.268593
TCAACGC23600.029.2204634
AACGCAG23400.029.1798636
CAACGCA23850.028.914175
GCCTGAC2900.028.1135887
ACGCAGA24600.027.7564567
CGCAGAG24650.027.5623428
AGAGTAC25450.026.6944911
CAGAGTA26050.025.95065910
AATAGAT16500.025.01397970
ATTCACT1551.8135324E-924.1186492
GCGTAGA20600.023.5901570
Produced by FastQC (version 0.11.5)