FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067754
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT112710.5450841831281671No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT93310.4512625776567232No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC84070.40657641092702523No Hit
GTTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAACT77990.37717252632566545No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC72190.349122767988842No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC67500.32644115305785887No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG62410.3018250720346811No Hit
CTGGTACCCCTGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT60230.29128223183221985No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGTATAATAACTGG59590.28818708608470833No Hit
GTCTGTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGCCTGGTA55700.2693744033381147No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATT55390.2678751921166638No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54470.2634259201046159No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT54060.26144309236011637No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT53330.25791269174186093No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT52090.2519158468560574No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCAGGGGTACCAGTGGCCCTGCT51670.24988465745925287No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA49200.2379393293399505No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG48870.23634339481388983No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAG48320.23368350393712214No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47790.23112033636496412No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG47730.2308301664511349No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG45660.22081930442402722No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG45600.22052913451019804No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT44670.2160315008458453No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT43660.21114697396305365No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC41910.20268368480970173No Hit
GAGTGTTACCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGTTGCATC41780.20205498332973845No Hit
GTCAGAGTGTTACCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGTTG41670.20152300515438487No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC38010.18382264041080323No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCAGGGGTACCA37450.18111438788173062No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGTTGCATCCAGCAGGGCCACTGGTACCCCTGCCAGGTT36030.17424703325443935No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC36000.17410194829752476No Hit
GGGTACCAGTGGCCCTGCTGGATGCAACAAAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG35690.17260273707607385No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA34760.16810510341172114No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34460.1666542538425751No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCCGCTCAGCTCCATGTAGG33230.16070577060907631No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33130.16022215408602766No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32890.1590614744307108No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32350.15644994520624794No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT32200.15572452042167492No Hit
CTCCCAGGCTCCTCATTTTTGTTGCATCCAGCAGGGCCACTGGTACCCCT31130.15054982362505404No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG30760.1487604424897739No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30200.14605218996070132No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT29930.14474642534846988No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29740.1438275539546774No Hit
CTGCTACTCTGGCTCCCAGAGACCAGTGGAGAAATAGTGATGACGCAGTC28110.13594460462898392No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27820.13454211671214275No Hit
GTTTATTTCTGTCAGCAGTATAATAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA27680.1338650535798746No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT27390.1324625656630334No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC27370.1323658423584237No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26390.12762640043254664No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG26290.12714278390949793No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA26210.126755890691059No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC26190.12665916738644925No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT26140.1264173591249249No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT26040.12593374260187623No Hit
CCTTGGTCCCTTGGCCGAACGTCGGAGGCCAGTTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGC26020.12583701929726648No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25870.12511159451269346No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT25840.12496650955577887No Hit
CCCTGTCTGTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGC25810.12482142459886428No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC25510.12337057502971824No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG25480.12322549007280363No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA25340.12254842694053548No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC25120.12148447058982839No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCAGTGGAGAAATAGTGATGACGCAGTCTC24900.12042051423912128No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC24600.11896966466997524No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA24430.11814751658079249No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT24160.11684175196856107No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC24130.11669666701164645No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24030.11621305048859779No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGGTA23820.11519745579019554No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT23780.11500400918097607No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGGTAACACTCTGACTGGCCCT23770.1149556475286712No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCAGTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC23640.11432694604870791No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA23620.11423022274409818No Hit
GTCTATTACTGTGCGAGATGTGATACAGCTGTGGCCACGCTCTTTGACTA23570.11398841448257385No Hit
GAAATAAACTGCAAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA23510.11369824456874462No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT22970.11108671534428177No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22590.1092489725566968No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22370.10818501620598968No Hit
TTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC22200.10736286811680693No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC22060.10668580498453878No Hit
GGCTCCCAGAGACCAGTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCGGGGGAAAGAG21900.10591201854766089No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGCCAGTT21840.10562184863383169No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21710.1049931471538684No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGTATAATAACTGGCCTCCGACGTT21670.10479970054464893No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGCCTGGTA21620.10455789228312458No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT21550.10421936071699052No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG21530.10412263741238079No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21470.10383246749855156No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT21130.10218817132018605No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21090.10199472471096659No Hit
GTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCG20960.1013660232310033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA205.44077E-499.797270
TCTAGCG200.00765585951.2364328
GTGGTAT3950.050.085141
GGTATCA15950.045.7647481
TGGTATC4250.044.938032
CGTATGC4350.042.8393741
TCGTATG4500.041.3628140
CGAGACG4550.040.4945823
ACGAGAC4600.040.04957622
ATCTCGT4250.039.61680637
ATGCCGT4750.039.36960644
CTTATAC4950.037.2105871
TACGTAT901.2410573E-436.9619270
GACGGAG4950.036.55067426
CCGTCTT5050.036.4623547
CCCACGA5100.036.11266319
CACGAGA5050.035.80090321
GTATGCC5450.035.50099642
GCTACAT5150.035.24212632
GGAGCTA5150.035.1695429
Produced by FastQC (version 0.11.5)